##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII Acc65I AclI AgeI AhdI
Aor51HI AscI Asp718I AspEI AviII
BbeI BbrPI BcgI BciVI BclI
BglI BglII BsaAI BsaBI BsiEI
BsiWI BsmBI BsmI BspDI BspHI
BsrBI BssHII BssSI BstBI BstEII
BstPI ClaI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV FbaI FseI FspI HaeII
HincII HindII HpaI KasI KpnI
KspI MamI MfeI MluI MunI
NarI NdeI NheI NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI Psp1406I PvuI RcaI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
Sse8387I SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AcyI 2116 GACGCC G[GA]^CG[CT]C
AflII 1712 CTTAAG C^TTAAG
Asp700I 3332 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 1666 CCTAGG C^CTAGG
BamHI 2960 GGATCC G^GATCC
BanI 2066 GGCACC G^G[CT][GA]CC
BbvCI 4631 GCTGAGG CCTCAGC(-5/-2)
BfrI 1712 CTTAAG C^TTAAG
BlnI 1666 CCTAGG C^CTAGG
BsaHI 2116 GACGCC G[GA]^CG[CT]C
Bsp1407I 3259 TGTACA T^GTACA
BsrDI 3111 CATTGC GCAATG(2/0)
BsrGI 3259 TGTACA T^GTACA
Bst1107I 3531 GTATAC GTA^TAC
BstZ17I 3531 GTATAC GTA^TAC
CpoI 2934 CGGTCCG CG^G[AT]CCG
DraIII 541 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoT22I 3920 ATGCAT ATGCA^T
HgaI 2116 GACGC GACGC(5/10)
Hin1I 2116 GACGCC G[GA]^CG[CT]C
NaeI 1555 GCCGGC GCC^GGC
NcoI 2120 CCATGG C^CATGG
NgoMIV 1555 GCCGGC G^CCGGC
NsiI 3920 ATGCAT ATGCA^T
PflMI 2835 CCAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1848 GACTTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 2934 CGGTCCG CG^G[AT]CCG
SpeI 2232 ACTAGT A^CTAGT
SphI 4598 GCATGC GCATG^C
SspBI 3259 TGTACA T^GTACA
StuI 1066 AGGCCT AGG^CCT
TaqI 4200 TCGA T^CGA
TthHB8I 4200 TCGA T^CGA
Van91I 2835 CCAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 4112 TCTAGA T^CTAGA
XmnI 3332 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccI 370 GTCTAC GT^[AC][GT]AC
3531 GTATAC
AccII 2489 CGCG CG^CG
4283 CGCG
AflIII 3409 ACATGT A^C[GA][CT]GT
4291 ACATGT
Alw44I 538 GTGCAC G^TGCAC
3138 GTGCAC
AocI 699 CCTGAGG CC^T[ACGT]AGG
2394 CCTAAGG
ApaI 600 GGGCCC GGGCC^C
2416 GGGCCC
ApaLI 538 GTGCAC G^TGCAC
3138 GTGCAC
AseI 942 ATTAAT AT^TAAT
2745 ATTAAT
AsnI 942 ATTAAT AT^TAAT
2745 ATTAAT
BbsI 3434 GAAGAC GAAGAC(2/6)
3443 GAAGAC
BlpI 156 GCTGAGC GC^T[ACGT]AGC
1696 GCTCAGC
Bpu1102I 156 GCTGAGC GC^T[ACGT]AGC
1696 GCTCAGC
BpuAI 3434 GAAGAC GAAGAC(2/6)
3443 GAAGAC
BsgI 4694 CTGCAC GTGCAG(16/14)
547 GTGCAG
BspLU11I 3409 ACATGT A^CATGT
4291 ACATGT
BspMI 2838 GCAGGT ACCTGC(4/8)
4498 GCAGGT
BsrFI 120 ACCGGC [GA]^CCGG[CT]
1555 GCCGGC
BstUI 2489 CGCG CG^CG
4283 CGCG
Bsu36I 699 CCTGAGG CC^T[ACGT]AGG
2394 CCTAAGG
CelII 156 GCTGAGC GC^T[ACGT]AGC
1696 GCTCAGC
CfoI 279 GCGC GCG^C
2488 GCGC
Cfr10I 120 ACCGGC [GA]^CCGG[CT]
1555 GCCGGC
DraI 2164 TTTAAA TTT^AAA
3911 TTTAAA
DsaI 151 CCGTGG C^C[GA][CT]GG
2120 CCATGG
Eco81I 699 CCTGAGG CC^T[ACGT]AGG
2394 CCTAAGG
HhaI 279 GCGC GCG^C
2488 GCGC
HinP1I 279 GCGC G^CGC
2488 GCGC
HindIII 262 AAGCTT A^AGCTT
2650 AAGCTT
MvnI 2489 CGCG CG^CG
4283 CGCG
PpuMI 1541 GGGTCCC [GA]G^G[AT]CC[CT]
1907 GGGACCT
PshBI 942 ATTAAT AT^TAAT
2745 ATTAAT
SacI 2442 GAGCTC GAGCT^C
3159 GAGCTC
SapI 1421 GAAGAGC GCTCTTC(1/4)
1260 GCTCTTC
SmaI 488 CCCGGG CCC^GGG
4705 CCCGGG
XmaCI 488 CCCGGG C^CCGGG
4705 CCCGGG
XmaI 488 CCCGGG C^CCGGG
4705 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccIII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AspI
AvaI AvaII BalI BanII BbvI
BcnI BfaI BmyI BpmI BsaI
BsaJI BsaWI BseAI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspEI BsrI BssKI BstAPI
BstF5I BstNI BstXI BstYI Cac8I
Cfr13I DdeI DpnII DraII EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MroI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflFI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
XcmI XhoII