##################################
# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI AlwI AlwNI
AocI Aor51HI ApaI AscI AseI
AsnI Asp718I AspEI AvaI AviII
AvrII BamHI BanII BbeI BbvCI
BcgI BciVI BclI BfrI BglII
BlnI BlpI Bpu1102I BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiWI BsmBI BsmI BsoBI
BspDI BspEI BspMI BsrBI BsrDI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstYI BstZ17I Bsu36I CelII
ClaI CpoI DpnII DraI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HaeII HgaI
Hin1I HincII HindII HpaI KasI
KpnI KspI MamI MboI MfeI
MflI MluI MroI MunI NarI
NcoI NdeII NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PleI PmeI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SacI SacII SalI SapI Sau3AI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I StuI StyI SwaI
Tsp45I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AccII 30 CGCG CG^CG
AflIII 776 ACATGT A^C[GA][CT]GT
Alw44I 719 GTGCAC G^TGCAC
ApaLI 719 GTGCAC G^TGCAC
Asp700I 172 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 719 GTGCAC G[AT]GC[AT]^C
AspI 763 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 273 TGGCCA TGG^CCA
BanI 483 GGTGCC G^G[CT][GA]CC
BbrPI 716 CACGTG CAC^GTG
BbsI 249 GAAGAC GAAGAC(2/6)
BcnI 392 CCCGG CC^[GC]GG
BglI 881 GCCTGGTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 719 GTGCAC G[AGT]GC[ACT]^C
BpuAI 249 GAAGAC GAAGAC(2/6)
BsiHKAI 719 GTGCAC G[AT]GC[AT]^C
BsiYI 386 CCCAGCCCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 386 CCCAGCCCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 1133 GTCCC GGGAC(10/14)
Bsp1286I 719 GTGCAC G[AGT]GC[ACT]^C
Bsp1407I 773 TGTACA T^GTACA
BspHI 728 TCATGA T^CATGA
BspLU11I 776 ACATGT A^CATGT
BsrFI 19 GCCGGC [GA]^CCGG[CT]
BsrGI 773 TGTACA T^GTACA
BstAPI 295 GCAGGCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 30 CGCG CG^CG
BstXI 1054 CCAAATTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 19 GCCGGC [GA]^CCGG[CT]
DsaI 52 CCGTGG C^C[GA][CT]GG
EaeI 273 TGGCCA [CT]^GGCC[GA]
EarI 330 GAAGAG CTCTTC(1/4)
FauI 23 GCGGG CCCGC(4/6)
HindIII 1123 AAGCTT A^AGCTT
Ksp632I 330 GAAGAG CTCTTC(1/4)
MaeIII 451 GTTAC ^GT[ACGT]AC
MluNI 273 TGGCCA TGG^CCA
MscI 273 TGGCCA TGG^CCA
MvnI 30 CGCG CG^CG
NaeI 19 GCCGGC GCC^GGC
NciI 392 CCCGG CC^[GC]GG
NdeI 661 CATATG CA^TATG
NgoMIV 19 GCCGGC G^CCGGC
NspI 776 ACATGT [GA]CATG^[CT]
PflFI 763 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 716 CACGTG CAC^GTG
PmlI 716 CACGTG CAC^GTG
RcaI 728 TCATGA T^CATGA
SexAI 479 ACCAGGT A^CC[AT]GGT
SspBI 773 TGTACA T^GTACA
SspI 191 AATATT AAT^ATT
Tth111I 763 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 172 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 1009 GTCTAC GT^[AC][GT]AC
1086 GTAGAC
AcsI 197 GAATTT [GA]^AATT[CT]
1056 AAATTT
AfaI 774 GTAC GT^AC
780 GTAC
ApoI 197 GAATTT [GA]^AATT[CT]
1056 AAATTT
BpmI 845 CTCCAG CTGGAG(16/14)
305 CTGGAG
BsaAI 103 CACGTA [CT]AC^GT[GA]
716 CACGTG
BsmAI 129 GAGAC GTCTC(1/5)
670 GAGAC
CfoI 29 GCGC GCG^C
574 GCGC
HapII 20 CCGG C^CGG
393 CCGG
HhaI 29 GCGC GCG^C
574 GCGC
HinP1I 29 GCGC G^CGC
574 GCGC
HpaII 20 CCGG C^CGG
393 CCGG
MaeII 104 ACGT A^CGT
717 ACGT
MslI 133 CACTTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1000 CATATTTATG
MspA1I 222 CCGCTG C[AC]G^C[GT]G
277 CAGCGG
MspI 20 CCGG C^CGG
393 CCGG
PstI 67 CTGCAG CTGCA^G
411 CTGCAG
RsaI 774 GTAC GT^AC
780 GTAC
SfaNI 292 GATGC GCATC(5/9)
619 GATGC
SfcI 67 CTGCAG C^T[GA][CT]AG
411 CTGCAG
TaqI 609 TCGA T^CGA
922 TCGA
TthHB8I 609 TCGA T^CGA
922 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AciI AluI AvaII BbvI BfaI
BsaJI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI EcoRII Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeI MboII MnlI MseI MvaI
MwoI NlaIII NlaIV Sau96I ScrFI
TfiI Tru9I TseI Tsp509I TspRI