##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Aor51HI AscI Asp700I
Asp718I AspEI AviII BbeI BbvCI
BcgI BfrI BglII BlpI Bpu1102I
BsaBI BsaWI BseAI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrGI BssHII Bst1107I
BstBI BstXI BstZ17I CelII ClaI
CpoI DraI EagI Eam1105I EclXI
Eco47III Eco52I EcoRV FseI FspI
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MroI MunI
NaeI NarI NgoMIV NheI NotI
NruI NspV PacI PflMI PinAI
PmeI PshAI Psp1406I PvuI RcaI
RsrII SacII SapI ScaI SfoI
SfuI SgrAI SnaBI SpeI SspBI
SspI StuI SwaI Van91I XbaI
XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccII 1483 CGCG CG^CG
AcyI 1720 GGCGTC G[GA]^CG[CT]C
AflIII 4110 ACACGT A^C[GA][CT]GT
Alw44I 2135 GTGCAC G^TGCAC
ApaLI 2135 GTGCAC G^TGCAC
AseI 1300 ATTAAT AT^TAAT
AsnI 1300 ATTAAT AT^TAAT
AspI 1823 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3037 CCTAGG C^CTAGG
BclI 4494 TGATCA T^GATCA
BlnI 3037 CCTAGG C^CTAGG
BsaHI 1720 GGCGTC G[GA]^CG[CT]C
BstAPI 2310 GCAAGGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1483 CGCG CG^CG
CfoI 3269 GCGC GCG^C
EarI 3170 GAAGAG CTCTTC(1/4)
EcoRI 4333 GAATTC G^AATTC
EcoT22I 4041 ATGCAT ATGCA^T
FbaI 4494 TGATCA T^GATCA
HaeII 3268 GGCGCT [GA]GCGC^[CT]
HgaI 1721 GCGTC GACGC(5/10)
HhaI 3269 GCGC GCG^C
Hin1I 1720 GGCGTC G[GA]^CG[CT]C
HinP1I 3269 GCGC G^CGC
Ksp632I 3170 GAAGAG CTCTTC(1/4)
MslI 1264 CACGCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 1483 CGCG CG^CG
NsiI 4041 ATGCAT ATGCA^T
PflFI 1823 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 1300 ATTAAT AT^TAAT
SalI 1820 GTCGAC G^TCGAC
SmaI 1373 CCCGGG CCC^GGG
Tth111I 1823 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1373 CCCGGG C^CCGGG
XmaI 1373 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 839 GTAGAC GT^[AC][GT]AC
1820 GTCGAC
BalI 2457 TGGCCA TGG^CCA
2916 TGGCCA
BamHI 310 GGATCC G^GATCC
1171 GGATCC
BbrPI 1472 CACGTG CAC^GTG
3407 CACGTG
BciVI 730 GGATAC GTATCC(6/5)
902 GGATAC
BsaAI 1472 CACGTG [CT]AC^GT[GA]
3407 CACGTG
BsrDI 3750 CATTGC GCAATG(2/0)
4263 CATTGC
BsrFI 1393 GCCGGT [GA]^CCGG[CT]
2131 GCCGGT
BssSI 2516 CTCGTG CACGAG(-5/-1)
2768 CTCGTG
BstEII 499 GGTGACC G^GT[ACGT]ACC
2800 GGTAACC
BstPI 499 GGTGACC G^GT[ACGT]ACC
2800 GGTAACC
Cfr10I 1393 GCCGGT [GA]^CCGG[CT]
2131 GCCGGT
EcoNI 2068 CCTGGTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3979 CCTAACCAAGG
EcoO65I 499 GGTGACC G^GT[ACGT]ACC
2800 GGTAACC
MluNI 2457 TGGCCA TGG^CCA
2916 TGGCCA
MscI 2457 TGGCCA TGG^CCA
2916 TGGCCA
NcoI 1031 CCATGG C^CATGG
4130 CCATGG
NdeI 608 CATATG CA^TATG
4581 CATATG
PaeR7I 1112 CTCGAG C^TCGAG
3632 CTCGAG
PmaCI 1472 CACGTG CAC^GTG
3407 CACGTG
PmlI 1472 CACGTG CAC^GTG
3407 CACGTG
SphI 1748 GCATGC GCATG^C
3026 GCATGC
XhoI 1112 CTCGAG C^TCGAG
3632 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApaI ApoI AspHI
AvaI AvaII BanI BanII BbsI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstYI
Bsu36I Cac8I Cfr13I DdeI DpnII
DraII DraIII DrdI DsaI EaeI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HincII
HindII HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
SbfI ScrFI SexAI SfaNI SfcI
SfiI SmlI Sse8387I StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII