##################################
# NO CUT ENZYMES ( 101 enzymes) #
##################################
AccII AccIII AflII AgeI AhdI
Aor51HI AscI AspEI AspI AvaI
AviII AvrII BamHI BbrPI BbvCI
BcgI BciVI BfrI BglI BlnI
BlpI Bpu1102I BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BsoBI BspDI
BspEI BsrBI BsrDI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstXI BstZ17I CelII Cfr10I ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoRI EcoRV
FauI FseI FspI KspI MfeI
MluI MroI MunI MvnI NaeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PvuI RsrII
SacI SacII SalI SbfI SexAI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I StuI
Tth111I XbaI XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 809 GACGTC GACGT^C
AccI 1351 GTCTAC GT^[AC][GT]AC
AclI 3866 AACGTT AA^CGTT
Alw44I 1722 GTGCAC G^TGCAC
AlwNI 116 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 121 CCTGAGG CC^T[ACGT]AGG
ApaI 1008 GGGCCC GGGCC^C
ApaLI 1722 GTGCAC G^TGCAC
AspHI 1722 GTGCAC G[AT]GC[AT]^C
BalI 3606 TGGCCA TGG^CCA
BanII 1008 GGGCCC G[GA]GC[CT]^C
BbeI 2238 GGCGCC GGCGC^C
BclI 561 TGATCA T^GATCA
BcnI 675 CCGGG CC^[GC]GG
BsaBI 2299 GATACAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 2724 GTGCAG GTGCAG(16/14)
BsiHKAI 1722 GTGCAC G[AT]GC[AT]^C
BsmI 1247 GCATTC GAATGC(1/-1)
BspHI 309 TCATGA T^CATGA
BspLU11I 4271 ACATGT A^CATGT
BstEII 225 GGTCACC G^GT[ACGT]ACC
BstPI 225 GGTCACC G^GT[ACGT]ACC
Bsu36I 121 CCTGAGG CC^T[ACGT]AGG
DraIII 1946 CACGTTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 121 CCTGAGG CC^T[ACGT]AGG
EcoO65I 225 GGTCACC G^GT[ACGT]ACC
FbaI 561 TGATCA T^GATCA
HgaI 1410 GCGTC GACGC(5/10)
HincII 2729 GTTAAC GT[CT]^[GA]AC
HindII 2729 GTTAAC GT[CT]^[GA]AC
HpaI 2729 GTTAAC GTT^AAC
KasI 2238 GGCGCC G^GCGCC
MamI 2299 GATACAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 3606 TGGCCA TGG^CCA
MscI 3606 TGGCCA TGG^CCA
MspA1I 4135 CAGCTG C[AC]G^C[GT]G
NarI 2238 GGCGCC GG^CGCC
NciI 675 CCGGG CC^[GC]GG
NdeI 980 CATATG CA^TATG
Psp1406I 3866 AACGTT AA^CGTT
PvuII 4135 CAGCTG CAG^CTG
RcaI 309 TCATGA T^CATGA
ScaI 3460 AGTACT AGT^ACT
SfoI 2238 GGCGCC GGC^GCC
SwaI 2380 ATTTAAAT ATTT^AAAT
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
Acc65I 375 GGTACC G^GTACC
2579 GGTACC
AcyI 809 GACGTC G[GA]^CG[CT]C
2238 GGCGCC
Asp700I 322 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3579 GAACCTTTTC
Asp718I 375 GGTACC G^GTACC
2579 GGTACC
BbsI 1422 GAAGAC GAAGAC(2/6)
4221 GTCTTC
BglII 300 AGATCT A^GATCT
333 AGATCT
BpmI 132 CTCCAG CTGGAG(16/14)
1337 CTGGAG
BpuAI 1422 GAAGAC GAAGAC(2/6)
4221 GTCTTC
BsaAI 1543 TACGTG [CT]AC^GT[GA]
2269 TACGTG
BsaHI 809 GACGTC G[GA]^CG[CT]C
2238 GGCGCC
BseRI 944 CTCCTC GAGGAG(10/8)
1450 GAGGAG
Bsp1407I 542 TGTACA T^GTACA
2020 TGTACA
BspMI 3488 ACCTGC ACCTGC(4/8)
2015 GCAGGT
BsrGI 542 TGTACA T^GTACA
2020 TGTACA
EaeI 923 CGGCCA [CT]^GGCC[GA]
3606 TGGCCA
EcoT22I 638 ATGCAT ATGCA^T
2592 ATGCAT
HaeII 2 GGCGCT [GA]GCGC^[CT]
2238 GGCGCC
HapII 675 CCGG C^CGG
1501 CCGG
Hin1I 809 GACGTC G[GA]^CG[CT]C
2238 GGCGCC
HindIII 933 AAGCTT A^AGCTT
2848 AAGCTT
HpaII 675 CCGG C^CGG
1501 CCGG
KpnI 375 GGTACC GGTAC^C
2579 GGTACC
MspI 675 CCGG C^CGG
1501 CCGG
NsiI 638 ATGCAT ATGCA^T
2592 ATGCAT
NspI 1496 ACATGC [GA]CATG^[CT]
4271 ACATGT
PflMI 2164 CCATAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4080 CCACTTTTTGG
PpuMI 1095 AGGACCC [GA]G^G[AT]CC[CT]
1839 GGGTCCT
PstI 495 CTGCAG CTGCA^G
2013 CTGCAG
SapI 177 GAAGAGC GCTCTTC(1/4)
1147 GCTCTTC
SspBI 542 TGTACA T^GTACA
2020 TGTACA
TaqI 1055 TCGA T^CGA
1194 TCGA
TthHB8I 1055 TCGA T^CGA
1194 TCGA
Van91I 2164 CCATAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4080 CCACTTTTTGG
XmnI 322 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3579 GAACCTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AseI AsnI AvaII
BanI BbvI BfaI BmyI BsaJI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HhaI HinP1I HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MvaI MwoI NcoI NdeII NlaIII
NlaIV PleI PshBI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SspI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII