##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI AocI
ApaI AscI AseI AsnI Asp718I
AspEI BbeI BbrPI BcgI BciVI
BclI BfrI BsaBI BsaHI BsaWI
BseAI BsiWI BsmBI BspDI BspEI
BspHI BspLU11I BssHII Bst1107I BstBI
BstZ17I Bsu36I ClaI CpoI DraI
Eam1105I Eco81I EcoRI EcoRV FbaI
FseI Hin1I HincII HindII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MunI NarI NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI ScaI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SwaI XhoI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccII 2700 CGCG CG^CG
AcsI 599 AAATTC [GA]^AATT[CT]
AflIII 3267 ACGTGT A^C[GA][CT]GT
Alw44I 1173 GTGCAC G^TGCAC
ApaLI 1173 GTGCAC G^TGCAC
ApoI 599 AAATTC [GA]^AATT[CT]
AspI 3879 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2827 TGCGCA TGC^GCA
AvrII 1080 CCTAGG C^CTAGG
BamHI 1468 GGATCC G^GATCC
BlnI 1080 CCTAGG C^CTAGG
BsiEI 2641 CGGCCG CG[GA][CT]^CG
BsrDI 4489 CATTGC GCAATG(2/0)
BsrFI 3627 GCCGGC [GA]^CCGG[CT]
BssSI 3123 CTCGTG CACGAG(-5/-1)
BstUI 2700 CGCG CG^CG
Cfr10I 3627 GCCGGC [GA]^CCGG[CT]
DraIII 362 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2843 GACAAGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2641 CGGCCG C^GGCCG
EclXI 2641 CGGCCG C^GGCCG
Eco52I 2641 CGGCCG C^GGCCG
EcoT22I 2666 ATGCAT ATGCA^T
FspI 2827 TGCGCA TGC^GCA
HgaI 3350 GCGTC GACGC(5/10)
HindIII 653 AAGCTT A^AGCTT
MvnI 2700 CGCG CG^CG
NaeI 3627 GCCGGC GCC^GGC
NdeI 106 CATATG CA^TATG
NgoMIV 3627 GCCGGC G^CCGGC
NheI 1154 GCTAGC G^CTAGC
NsiI 2666 ATGCAT ATGCA^T
PflFI 3879 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 2131 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 3413 GAAGAGC GCTCTTC(1/4)
SexAI 609 ACCAGGT A^CC[AT]GGT
SfiI 3725 GGCCAGAAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 2737 GCATGC GCATG^C
SspI 4255 AATATT AAT^ATT
Tth111I 3879 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4362 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Aor51HI 443 AGCGCT AGC^GCT
3512 AGCGCT
Asp700I 1022 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2026 GAATCAGTTC
BalI 868 TGGCCA TGG^CCA
3480 TGGCCA
BglII 703 AGATCT A^GATCT
2436 AGATCT
BlpI 578 GCTCAGC GC^T[ACGT]AGC
4515 GCTAAGC
Bpu1102I 578 GCTCAGC GC^T[ACGT]AGC
4515 GCTAAGC
BsaAI 1811 TACGTG [CT]AC^GT[GA]
3266 TACGTG
Bsp1407I 369 TGTACA T^GTACA
1246 TGTACA
BsrGI 369 TGTACA T^GTACA
1246 TGTACA
BstAPI 2668 GCATCATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3532 GCAGGAGCTGC
CelII 578 GCTCAGC GC^T[ACGT]AGC
4515 GCTAAGC
Eco47III 443 AGCGCT AGC^GCT
3512 AGCGCT
MluNI 868 TGGCCA TGG^CCA
3480 TGGCCA
MscI 868 TGGCCA TGG^CCA
3480 TGGCCA
NcoI 635 CCATGG C^CATGG
2338 CCATGG
NspI 2737 GCATGC [GA]CATG^[CT]
3303 ACATGC
PflMI 2302 CCACATTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4241 CCAGGTATTGG
SacI 734 GAGCTC GAGCT^C
3227 GAGCTC
SmaI 1550 CCCGGG CCC^GGG
3951 CCCGGG
SmlI 3170 CTTGAG C^T[CT][GA]AG
3638 CTCAAG
SspBI 369 TGTACA T^GTACA
1246 TGTACA
Van91I 2302 CCACATTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4241 CCAGGTATTGG
XmaCI 1550 CCCGGG C^CCGGG
3951 CCCGGG
XmaI 1550 CCCGGG C^CCGGG
3951 CCCGGG
XmnI 1022 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2026 GAATCAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaI AvaII BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrBI BsrI BssKI
BstEII BstF5I BstNI BstPI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII