################################## # NO CUT ENZYMES ( 72 enzymes) # ################################## AccI AclI AflII AflIII AgeI AhdI Aor51HI AscI AseI AsnI Asp700I AspEI AviII BciVI BclI BfrI BglII BsaBI BsiWI Bsp1407I BspDI BspHI BspLU11I BsrGI BssHII Bst1107I BstBI BstZ17I ClaI DraI DrdI Eam1105I Eco47III EcoRI EcoRV EcoT22I FbaI FspI HindIII KspI MamI MluI NdeI NotI NruI NsiI NspV PacI PinAI PmeI PshBI Psp1406I PvuI RcaI SacII SalI SbfI ScaI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AatII 4044 GACGTC GACGT^C Acc65I 2591 GGTACC G^GTACC AccIII 4001 TCCGGA T^CCGGA Asp718I 2591 GGTACC G^GTACC AspI 569 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 957 GGATCC G^GATCC BbrPI 1499 CACGTG CAC^GTG BlpI 121 GCTGAGC GC^T[ACGT]AGC Bpu1102I 121 GCTGAGC GC^T[ACGT]AGC BsaAI 1499 CACGTG [CT]AC^GT[GA] BsaWI 4001 TCCGGA [AT]^CCGG[AT] BseAI 4001 TCCGGA T^CCGGA BsiEI 4073 CGGCCG CG[GA][CT]^CG BsmBI 567 GAGACG CGTCTC(1/5) BspEI 4001 TCCGGA T^CCGGA CelII 121 GCTGAGC GC^T[ACGT]AGC CpoI 364 CGGTCCG CG^G[AT]CCG EagI 4073 CGGCCG C^GGCCG EclXI 4073 CGGCCG C^GGCCG Eco52I 4073 CGGCCG C^GGCCG HpaI 778 GTTAAC GTT^AAC KpnI 2591 GGTACC GGTAC^C MroI 4001 TCCGGA T^CCGGA NheI 2799 GCTAGC G^CTAGC PaeR7I 461 CTCGAG C^TCGAG PflFI 569 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 2056 GACTC GAGTC(4/5) PmaCI 1499 CACGTG CAC^GTG PmlI 1499 CACGTG CAC^GTG RsrII 364 CGGTCCG CG^G[AT]CCG SapI 206 GAAGAGC GCTCTTC(1/4) SfiI 4079 GGCCGTGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC Tth111I 569 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 461 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AcsI 197 AAATTT [GA]^AATT[CT] 785 AAATTC Alw44I 542 GTGCAC G^TGCAC 1496 GTGCAC AocI 1138 CCTGAGG CC^T[ACGT]AGG 1459 CCTCAGG ApaLI 542 GTGCAC G^TGCAC 1496 GTGCAC ApoI 197 AAATTT [GA]^AATT[CT] 785 AAATTC BsmI 3024 GCATTC GAATGC(1/-1) 3032 GCATTC BstEII 1029 GGTGACC G^GT[ACGT]ACC 3270 GGTGACC BstPI 1029 GGTGACC G^GT[ACGT]ACC 3270 GGTGACC Bsu36I 1138 CCTGAGG CC^T[ACGT]AGG 1459 CCTCAGG Eco81I 1138 CCTGAGG CC^T[ACGT]AGG 1459 CCTCAGG EcoO65I 1029 GGTGACC G^GT[ACGT]ACC 3270 GGTGACC FseI 263 GGCCGGCC GGCCGG^CC 2813 GGCCGGCC HincII 778 GTTAAC GT[CT]^[GA]AC 3512 GTCAAC HindII 778 GTTAAC GT[CT]^[GA]AC 3512 GTCAAC MfeI 527 CAATTG C^AATTG 721 CAATTG MunI 527 CAATTG C^AATTG 721 CAATTG NaeI 264 GCCGGC GCC^GGC 2814 GCCGGC NgoMIV 264 GCCGGC G^CCGGC 2814 GCCGGC PshAI 34 GACTGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 2632 GACTGCTGTC SexAI 2305 ACCAGGT A^CC[AT]GGT 2966 ACCAGGT ######################################### # 3 OR MORE CUT ENZYMES ( 132 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI ApaI AspHI AvaI AvaII AvrII BalI BanI BanII BbeI BbsI BbvCI BbvI BcgI BcnI BfaI BglI BlnI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII