##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AccI AclI AflII AflIII AgeI
AhdI Aor51HI AscI AseI AsnI
Asp700I AspEI AviII BciVI BclI
BfrI BglII BsaBI BsiWI Bsp1407I
BspDI BspHI BspLU11I BsrGI BssHII
Bst1107I BstBI BstZ17I ClaI DraI
DrdI Eam1105I Eco47III EcoRI EcoRV
EcoT22I FbaI FspI HindIII KspI
MamI MluI NdeI NotI NruI
NsiI NspV PacI PinAI PmeI
PshBI Psp1406I PvuI RcaI SacII
SalI SbfI ScaI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI XbaI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AatII 4044 GACGTC GACGT^C
Acc65I 2591 GGTACC G^GTACC
AccIII 4001 TCCGGA T^CCGGA
Asp718I 2591 GGTACC G^GTACC
AspI 569 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 957 GGATCC G^GATCC
BbrPI 1499 CACGTG CAC^GTG
BlpI 121 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 121 GCTGAGC GC^T[ACGT]AGC
BsaAI 1499 CACGTG [CT]AC^GT[GA]
BsaWI 4001 TCCGGA [AT]^CCGG[AT]
BseAI 4001 TCCGGA T^CCGGA
BsiEI 4073 CGGCCG CG[GA][CT]^CG
BsmBI 567 GAGACG CGTCTC(1/5)
BspEI 4001 TCCGGA T^CCGGA
CelII 121 GCTGAGC GC^T[ACGT]AGC
CpoI 364 CGGTCCG CG^G[AT]CCG
EagI 4073 CGGCCG C^GGCCG
EclXI 4073 CGGCCG C^GGCCG
Eco52I 4073 CGGCCG C^GGCCG
HpaI 778 GTTAAC GTT^AAC
KpnI 2591 GGTACC GGTAC^C
MroI 4001 TCCGGA T^CCGGA
NheI 2799 GCTAGC G^CTAGC
PaeR7I 461 CTCGAG C^TCGAG
PflFI 569 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 2056 GACTC GAGTC(4/5)
PmaCI 1499 CACGTG CAC^GTG
PmlI 1499 CACGTG CAC^GTG
RsrII 364 CGGTCCG CG^G[AT]CCG
SapI 206 GAAGAGC GCTCTTC(1/4)
SfiI 4079 GGCCGTGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Tth111I 569 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 461 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcsI 197 AAATTT [GA]^AATT[CT]
785 AAATTC
Alw44I 542 GTGCAC G^TGCAC
1496 GTGCAC
AocI 1138 CCTGAGG CC^T[ACGT]AGG
1459 CCTCAGG
ApaLI 542 GTGCAC G^TGCAC
1496 GTGCAC
ApoI 197 AAATTT [GA]^AATT[CT]
785 AAATTC
BsmI 3024 GCATTC GAATGC(1/-1)
3032 GCATTC
BstEII 1029 GGTGACC G^GT[ACGT]ACC
3270 GGTGACC
BstPI 1029 GGTGACC G^GT[ACGT]ACC
3270 GGTGACC
Bsu36I 1138 CCTGAGG CC^T[ACGT]AGG
1459 CCTCAGG
Eco81I 1138 CCTGAGG CC^T[ACGT]AGG
1459 CCTCAGG
EcoO65I 1029 GGTGACC G^GT[ACGT]ACC
3270 GGTGACC
FseI 263 GGCCGGCC GGCCGG^CC
2813 GGCCGGCC
HincII 778 GTTAAC GT[CT]^[GA]AC
3512 GTCAAC
HindII 778 GTTAAC GT[CT]^[GA]AC
3512 GTCAAC
MfeI 527 CAATTG C^AATTG
721 CAATTG
MunI 527 CAATTG C^AATTG
721 CAATTG
NaeI 264 GCCGGC GCC^GGC
2814 GCCGGC
NgoMIV 264 GCCGGC G^CCGGC
2814 GCCGGC
PshAI 34 GACTGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2632 GACTGCTGTC
SexAI 2305 ACCAGGT A^CC[AT]GGT
2966 ACCAGGT
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII AvrII BalI BanI BanII
BbeI BbsI BbvCI BbvI BcgI
BcnI BfaI BglI BlnI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII