##################################
# NO CUT ENZYMES (  107 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AgeI        AhdI        Alw44I      Aor51HI     ApaI        
ApaLI       AscI        Asp718I     AspEI       AvaI        
AviII       AvrII       BamHI       BbeI        BbrPI       
BbvCI       BcgI        BcnI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BsiWI       BsmBI       
BsoBI       Bsp1407I    BspDI       BspEI       BsrBI       
BsrFI       BsrGI       BssHII      Bst1107I    BstBI       
BstXI       BstZ17I     CelII       Cfr10I      ClaI        
CpoI        DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      FseI        FspI        
HaeII       HgaI        Hin1I       KasI        KpnI        
KspI        MamI        MluI        MroI        NaeI        
NarI        NciI        NdeI        NgoMIV      NheI        
NotI        NspV        PacI        PaeR7I      PflMI       
PinAI       PmaCI       PmeI        PmlI        Psp1406I    
PvuI        RsrII       SacI        SacII       SalI        
SbfI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SphI        Sse8387I    
SspBI       StuI        SwaI        Van91I      XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AccI          711  GTCTAC               GT^[AC][GT]AC
AflII        3795  CTTAAG               C^TTAAG
AflIII        300  ACATGT               A^C[GA][CT]GT
AocI         1790  CCTCAGG              CC^T[ACGT]AGG
AseI         2034  ATTAAT               AT^TAAT
AsnI         2034  ATTAAT               AT^TAAT
BciVI        3144  GGATAC               GTATCC(6/5)
BfrI         3795  CTTAAG               C^TTAAG
BsaAI         458  CACGTA               [CT]AC^GT[GA]
BseRI         655  GAGGAG               GAGGAG(10/8)
BsiEI        1216  CGACCG               CG[GA][CT]^CG
BsmI         3317  GCATTC               GAATGC(1/-1)
BspLU11I      300  ACATGT               A^CATGT
BspMI        1810  ACCTGC               ACCTGC(4/8)
BsrDI        4069  GCAATG               GCAATG(2/0)
BssSI        2837  CTCGTG               CACGAG(-5/-1)
BstEII        107  GGTGACC              G^GT[ACGT]ACC
BstPI         107  GGTGACC              G^GT[ACGT]ACC
BstYI        1332  AGATCC               [GA]^GATC[CT]
Bsu36I       1790  CCTCAGG              CC^T[ACGT]AGG
Eco81I       1790  CCTCAGG              CC^T[ACGT]AGG
EcoO65I       107  GGTGACC              G^GT[ACGT]ACC
EcoRV        3686  GATATC               GAT^ATC
FauI          104  GCGGG                CCCGC(4/6)
HapII        1261  CCGG                 C^CGG
HpaII        1261  CCGG                 C^CGG
MaeII         459  ACGT                 A^CGT
MfeI          905  CAATTG               C^AATTG
MflI         1332  AGATCC               [GA]^GATC[CT]
MspI         1261  CCGG                 C^CGG
MunI          905  CAATTG               C^AATTG
NcoI         1043  CCATGG               C^CATGG
NruI            2  TCGCGA               TCG^CGA
PshAI        1217  GACCGCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        2034  ATTAAT               AT^TAAT
SapI         1316  GAAGAGC              GCTCTTC(1/4)
ScaI          868  AGTACT               AGT^ACT
SpeI          127  ACTAGT               A^CTAGT
XbaI          609  TCTAGA               T^CTAGA
XcmI         1293  CCAAAGTACAAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII        1332  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   36 enzymes) #
###################################

AlwNI        1691  CAGAAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1739  CAGAGCCTG           
AspHI          91  GTGCTC               G[AT]GC[AT]^C
             2046  GTGCTC              
AspI          466  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1197  GACTCAGTC           
BalI          381  TGGCCA               TGG^CCA
             3016  TGGCCA              
BanI          885  GGCACC               G^G[CT][GA]CC
             1047  GGTGCC              
BanII          28  GAGCCC               G[GA]GC[CT]^C
               98  GGGCTC              
BbsI         1438  GAAGAC               GAAGAC(2/6)
             1600  GAAGAC              
BclI          483  TGATCA               T^GATCA
             1959  TGATCA              
BpuAI        1438  GAAGAC               GAAGAC(2/6)
             1600  GAAGAC              
BsiHKAI        91  GTGCTC               G[AT]GC[AT]^C
             2046  GTGCTC              
BspHI         121  TCATGA               T^CATGA
             3676  TCATGA              
CfoI          370  GCGC                 GCG^C
             2938  GCGC                
DraII         326  AGGACCT              [GA]G^G[ACGT]CC[CT]
             1071  AGGTCCT             
DsaI           32  CCGTGG               C^C[GA][CT]GG
             1043  CCATGG              
EaeI          381  TGGCCA               [CT]^GGCC[GA]
             3016  TGGCCA              
EarI          932  CTCTTC               CTCTTC(1/4)
             1316  GAAGAG              
EcoNI         893  CCTCTAACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              913  CCTGTACCAGG         
EcoO109I      326  AGGACCT              [GA]G^G[ACGT]CC[CT]
             1071  AGGTCCT             
EcoT22I      2680  ATGCAT               ATGCA^T
             3353  ATGCAT              
FbaI          483  TGATCA               T^GATCA
             1959  TGATCA              
HhaI          370  GCGC                 GCG^C
             2938  GCGC                
HinP1I        370  GCGC                 G^CGC
             2938  GCGC                
HindIII       578  AAGCTT               A^AGCTT
             3921  AAGCTT              
HpaI         2245  GTTAAC               GTT^AAC
             3915  GTTAAC              
Ksp632I       932  CTCTTC               CTCTTC(1/4)
             1316  GAAGAG              
MluNI         381  TGGCCA               TGG^CCA
             3016  TGGCCA              
MscI          381  TGGCCA               TGG^CCA
             3016  TGGCCA              
NsiI         2680  ATGCAT               ATGCA^T
             3353  ATGCAT              
NspI          300  ACATGT               [GA]CATG^[CT]
             1005  GCATGT              
PflFI         466  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1197  GACTCAGTC           
PpuMI         326  AGGACCT              [GA]G^G[AT]CC[CT]
             1071  AGGTCCT             
RcaI          121  TCATGA               T^CATGA
             3676  TCATGA              
SspI         2019  AATATT               AAT^ATT
             2526  AATATT              
TaqI         1032  TCGA                 T^CGA
             1215  TCGA                
Tth111I       466  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1197  GACTCAGTC           
TthHB8I      1032  TCGA                 T^CGA
             1215  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   74 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        ApoI        Asp700I     AvaII       BbvI        
BfaI        BmyI        BpmI        BsaJI       BsgI        
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstUI       Cac8I       Cfr13I      DdeI        DpnII       
DraI        EcoRI       EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HincII      HindII      HinfI       
HphI        ItaI        MaeI        MaeIII      MboI        
MboII       MnlI        MseI        MslI        MspA1I      
MvaI        MvnI        MwoI        NdeII       NlaIII      
NlaIV       PleI        PstI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        StyI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       XmnI