################################## # NO CUT ENZYMES ( 107 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI Asp718I AspEI AvaI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BcnI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BsrBI BsrFI BsrGI BssHII Bst1107I BstBI BstXI BstZ17I CelII Cfr10I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I FseI FspI HaeII HgaI Hin1I KasI KpnI KspI MamI MluI MroI NaeI NarI NciI NdeI NgoMIV NheI NotI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SacI SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SspBI StuI SwaI Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccI 711 GTCTAC GT^[AC][GT]AC AflII 3795 CTTAAG C^TTAAG AflIII 300 ACATGT A^C[GA][CT]GT AocI 1790 CCTCAGG CC^T[ACGT]AGG AseI 2034 ATTAAT AT^TAAT AsnI 2034 ATTAAT AT^TAAT BciVI 3144 GGATAC GTATCC(6/5) BfrI 3795 CTTAAG C^TTAAG BsaAI 458 CACGTA [CT]AC^GT[GA] BseRI 655 GAGGAG GAGGAG(10/8) BsiEI 1216 CGACCG CG[GA][CT]^CG BsmI 3317 GCATTC GAATGC(1/-1) BspLU11I 300 ACATGT A^CATGT BspMI 1810 ACCTGC ACCTGC(4/8) BsrDI 4069 GCAATG GCAATG(2/0) BssSI 2837 CTCGTG CACGAG(-5/-1) BstEII 107 GGTGACC G^GT[ACGT]ACC BstPI 107 GGTGACC G^GT[ACGT]ACC BstYI 1332 AGATCC [GA]^GATC[CT] Bsu36I 1790 CCTCAGG CC^T[ACGT]AGG Eco81I 1790 CCTCAGG CC^T[ACGT]AGG EcoO65I 107 GGTGACC G^GT[ACGT]ACC EcoRV 3686 GATATC GAT^ATC FauI 104 GCGGG CCCGC(4/6) HapII 1261 CCGG C^CGG HpaII 1261 CCGG C^CGG MaeII 459 ACGT A^CGT MfeI 905 CAATTG C^AATTG MflI 1332 AGATCC [GA]^GATC[CT] MspI 1261 CCGG C^CGG MunI 905 CAATTG C^AATTG NcoI 1043 CCATGG C^CATGG NruI 2 TCGCGA TCG^CGA PshAI 1217 GACCGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 2034 ATTAAT AT^TAAT SapI 1316 GAAGAGC GCTCTTC(1/4) ScaI 868 AGTACT AGT^ACT SpeI 127 ACTAGT A^CTAGT XbaI 609 TCTAGA T^CTAGA XcmI 1293 CCAAAGTACAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 1332 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### AlwNI 1691 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG 1739 CAGAGCCTG AspHI 91 GTGCTC G[AT]GC[AT]^C 2046 GTGCTC AspI 466 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1197 GACTCAGTC BalI 381 TGGCCA TGG^CCA 3016 TGGCCA BanI 885 GGCACC G^G[CT][GA]CC 1047 GGTGCC BanII 28 GAGCCC G[GA]GC[CT]^C 98 GGGCTC BbsI 1438 GAAGAC GAAGAC(2/6) 1600 GAAGAC BclI 483 TGATCA T^GATCA 1959 TGATCA BpuAI 1438 GAAGAC GAAGAC(2/6) 1600 GAAGAC BsiHKAI 91 GTGCTC G[AT]GC[AT]^C 2046 GTGCTC BspHI 121 TCATGA T^CATGA 3676 TCATGA CfoI 370 GCGC GCG^C 2938 GCGC DraII 326 AGGACCT [GA]G^G[ACGT]CC[CT] 1071 AGGTCCT DsaI 32 CCGTGG C^C[GA][CT]GG 1043 CCATGG EaeI 381 TGGCCA [CT]^GGCC[GA] 3016 TGGCCA EarI 932 CTCTTC CTCTTC(1/4) 1316 GAAGAG EcoNI 893 CCTCTAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 913 CCTGTACCAGG EcoO109I 326 AGGACCT [GA]G^G[ACGT]CC[CT] 1071 AGGTCCT EcoT22I 2680 ATGCAT ATGCA^T 3353 ATGCAT FbaI 483 TGATCA T^GATCA 1959 TGATCA HhaI 370 GCGC GCG^C 2938 GCGC HinP1I 370 GCGC G^CGC 2938 GCGC HindIII 578 AAGCTT A^AGCTT 3921 AAGCTT HpaI 2245 GTTAAC GTT^AAC 3915 GTTAAC Ksp632I 932 CTCTTC CTCTTC(1/4) 1316 GAAGAG MluNI 381 TGGCCA TGG^CCA 3016 TGGCCA MscI 381 TGGCCA TGG^CCA 3016 TGGCCA NsiI 2680 ATGCAT ATGCA^T 3353 ATGCAT NspI 300 ACATGT [GA]CATG^[CT] 1005 GCATGT PflFI 466 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1197 GACTCAGTC PpuMI 326 AGGACCT [GA]G^G[AT]CC[CT] 1071 AGGTCCT RcaI 121 TCATGA T^CATGA 3676 TCATGA SspI 2019 AATATT AAT^ATT 2526 AATATT TaqI 1032 TCGA T^CGA 1215 TCGA Tth111I 466 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1197 GACTCAGTC TthHB8I 1032 TCGA T^CGA 1215 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 74 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI ApoI Asp700I AvaII BbvI BfaI BmyI BpmI BsaJI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstAPI BstF5I BstNI BstUI Cac8I Cfr13I DdeI DpnII DraI EcoRI EcoRII EcoT14I Fnu4HI FokI HaeIII HincII HindII HinfI HphI ItaI MaeI MaeIII MboI MboII MnlI MseI MslI MspA1I MvaI MvnI MwoI NdeII NlaIII NlaIV PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XmnI