##################################
# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI Asp718I AspEI AvaI
AviII AvrII BamHI BbeI BbrPI
BbvCI BcgI BcnI BglI BglII
BlnI BlpI Bpu1102I BsaBI BsaHI
BsaI BsaWI BseAI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BsrBI
BsrFI BsrGI BssHII Bst1107I BstBI
BstXI BstZ17I CelII Cfr10I ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I FseI FspI
HaeII HgaI Hin1I KasI KpnI
KspI MamI MluI MroI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
SspBI StuI SwaI Van91I XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccI 711 GTCTAC GT^[AC][GT]AC
AflII 3795 CTTAAG C^TTAAG
AflIII 300 ACATGT A^C[GA][CT]GT
AocI 1790 CCTCAGG CC^T[ACGT]AGG
AseI 2034 ATTAAT AT^TAAT
AsnI 2034 ATTAAT AT^TAAT
BciVI 3144 GGATAC GTATCC(6/5)
BfrI 3795 CTTAAG C^TTAAG
BsaAI 458 CACGTA [CT]AC^GT[GA]
BseRI 655 GAGGAG GAGGAG(10/8)
BsiEI 1216 CGACCG CG[GA][CT]^CG
BsmI 3317 GCATTC GAATGC(1/-1)
BspLU11I 300 ACATGT A^CATGT
BspMI 1810 ACCTGC ACCTGC(4/8)
BsrDI 4069 GCAATG GCAATG(2/0)
BssSI 2837 CTCGTG CACGAG(-5/-1)
BstEII 107 GGTGACC G^GT[ACGT]ACC
BstPI 107 GGTGACC G^GT[ACGT]ACC
BstYI 1332 AGATCC [GA]^GATC[CT]
Bsu36I 1790 CCTCAGG CC^T[ACGT]AGG
Eco81I 1790 CCTCAGG CC^T[ACGT]AGG
EcoO65I 107 GGTGACC G^GT[ACGT]ACC
EcoRV 3686 GATATC GAT^ATC
FauI 104 GCGGG CCCGC(4/6)
HapII 1261 CCGG C^CGG
HpaII 1261 CCGG C^CGG
MaeII 459 ACGT A^CGT
MfeI 905 CAATTG C^AATTG
MflI 1332 AGATCC [GA]^GATC[CT]
MspI 1261 CCGG C^CGG
MunI 905 CAATTG C^AATTG
NcoI 1043 CCATGG C^CATGG
NruI 2 TCGCGA TCG^CGA
PshAI 1217 GACCGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2034 ATTAAT AT^TAAT
SapI 1316 GAAGAGC GCTCTTC(1/4)
ScaI 868 AGTACT AGT^ACT
SpeI 127 ACTAGT A^CTAGT
XbaI 609 TCTAGA T^CTAGA
XcmI 1293 CCAAAGTACAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1332 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AlwNI 1691 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1739 CAGAGCCTG
AspHI 91 GTGCTC G[AT]GC[AT]^C
2046 GTGCTC
AspI 466 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1197 GACTCAGTC
BalI 381 TGGCCA TGG^CCA
3016 TGGCCA
BanI 885 GGCACC G^G[CT][GA]CC
1047 GGTGCC
BanII 28 GAGCCC G[GA]GC[CT]^C
98 GGGCTC
BbsI 1438 GAAGAC GAAGAC(2/6)
1600 GAAGAC
BclI 483 TGATCA T^GATCA
1959 TGATCA
BpuAI 1438 GAAGAC GAAGAC(2/6)
1600 GAAGAC
BsiHKAI 91 GTGCTC G[AT]GC[AT]^C
2046 GTGCTC
BspHI 121 TCATGA T^CATGA
3676 TCATGA
CfoI 370 GCGC GCG^C
2938 GCGC
DraII 326 AGGACCT [GA]G^G[ACGT]CC[CT]
1071 AGGTCCT
DsaI 32 CCGTGG C^C[GA][CT]GG
1043 CCATGG
EaeI 381 TGGCCA [CT]^GGCC[GA]
3016 TGGCCA
EarI 932 CTCTTC CTCTTC(1/4)
1316 GAAGAG
EcoNI 893 CCTCTAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
913 CCTGTACCAGG
EcoO109I 326 AGGACCT [GA]G^G[ACGT]CC[CT]
1071 AGGTCCT
EcoT22I 2680 ATGCAT ATGCA^T
3353 ATGCAT
FbaI 483 TGATCA T^GATCA
1959 TGATCA
HhaI 370 GCGC GCG^C
2938 GCGC
HinP1I 370 GCGC G^CGC
2938 GCGC
HindIII 578 AAGCTT A^AGCTT
3921 AAGCTT
HpaI 2245 GTTAAC GTT^AAC
3915 GTTAAC
Ksp632I 932 CTCTTC CTCTTC(1/4)
1316 GAAGAG
MluNI 381 TGGCCA TGG^CCA
3016 TGGCCA
MscI 381 TGGCCA TGG^CCA
3016 TGGCCA
NsiI 2680 ATGCAT ATGCA^T
3353 ATGCAT
NspI 300 ACATGT [GA]CATG^[CT]
1005 GCATGT
PflFI 466 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1197 GACTCAGTC
PpuMI 326 AGGACCT [GA]G^G[AT]CC[CT]
1071 AGGTCCT
RcaI 121 TCATGA T^CATGA
3676 TCATGA
SspI 2019 AATATT AAT^ATT
2526 AATATT
TaqI 1032 TCGA T^CGA
1215 TCGA
Tth111I 466 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1197 GACTCAGTC
TthHB8I 1032 TCGA T^CGA
1215 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI Asp700I AvaII BbvI
BfaI BmyI BpmI BsaJI BsgI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstAPI BstF5I BstNI
BstUI Cac8I Cfr13I DdeI DpnII
DraI EcoRI EcoRII EcoT14I Fnu4HI
FokI HaeIII HincII HindII HinfI
HphI ItaI MaeI MaeIII MboI
MboII MnlI MseI MslI MspA1I
MvaI MvnI MwoI NdeII NlaIII
NlaIV PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XmnI