##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII Acc65I AclI AcyI AflIII
AgeI AhdI AscI Asp718I AspEI
AviII BbeI BbrPI BbsI BglI
BglII BpuAI BsaAI BsaBI BsaHI
BsiEI BsiWI BspDI BspLU11I BssHII
BssSI ClaI CpoI DraIII DrdI
EagI Eam1105I EclXI Eco52I EcoRV
FseI FspI HgaI Hin1I KasI
KpnI KspI MamI MfeI MluI
MunI NarI NdeI NheI NotI
NruI PacI PaeR7I PinAI PmaCI
PmlI PshAI Psp1406I PvuI RsrII
SacI SacII SalI SbfI SexAI
SfiI SfoI SgrAI SnaBI SpeI
Sse8387I SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccIII 139 TCCGGA T^CCGGA
AflII 1502 CTTAAG C^TTAAG
Alw44I 696 GTGCAC G^TGCAC
ApaLI 696 GTGCAC G^TGCAC
Asp700I 721 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 136 GGATCC G^GATCC
BanI 1320 GGCACC G^G[CT][GA]CC
BclI 2426 TGATCA T^GATCA
BfrI 1502 CTTAAG C^TTAAG
BseAI 139 TCCGGA T^CCGGA
BsmBI 43 CGTCTC CGTCTC(1/5)
BspEI 139 TCCGGA T^CCGGA
BspHI 313 TCATGA T^CATGA
Bst1107I 2273 GTATAC GTA^TAC
BstBI 728 TTCGAA TT^CGAA
BstEII 304 GGTCACC G^GT[ACGT]ACC
BstPI 304 GGTCACC G^GT[ACGT]ACC
BstZ17I 2273 GTATAC GTA^TAC
EcoO65I 304 GGTCACC G^GT[ACGT]ACC
EcoRI 3190 GAATTC G^AATTC
EcoT22I 3687 ATGCAT ATGCA^T
FbaI 2426 TGATCA T^GATCA
HindIII 1505 AAGCTT A^AGCTT
HpaI 3569 GTTAAC GTT^AAC
MaeII 624 ACGT A^CGT
MroI 139 TCCGGA T^CCGGA
MslI 3459 CATGCCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NsiI 3687 ATGCAT ATGCA^T
NspV 728 TTCGAA TT^CGAA
PflMI 199 CCACATTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmeI 3120 GTTTAAAC GTTT^AAAC
RcaI 313 TCATGA T^CATGA
ScaI 381 AGTACT AGT^ACT
SfuI 728 TTCGAA TT^CGAA
SphI 3458 GCATGC GCATG^C
Van91I 199 CCACATTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2410 TCTAGA T^CTAGA
XmnI 721 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 743 CGCG CG^CG
3935 CGCG
AlwNI 459 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
4003 CAGTTCCTG
Aor51HI 1200 AGCGCT AGC^GCT
2710 AGCGCT
ApaI 668 GGGCCC GGGCC^C
3400 GGGCCC
AseI 2903 ATTAAT AT^TAAT
2918 ATTAAT
AsnI 2903 ATTAAT AT^TAAT
2918 ATTAAT
AvrII 3834 CCTAGG C^CTAGG
4037 CCTAGG
BcgI 217 CGAAGGGCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3822 GCATTTTCCTCG
BciVI 1022 GTATCC GTATCC(6/5)
3084 GTATCC
BlnI 3834 CCTAGG C^CTAGG
4037 CCTAGG
BsaWI 139 TCCGGA [AT]^CCGG[AT]
3257 TCCGGT
Bsp1407I 2923 TGTACA T^GTACA
3563 TGTACA
BspMI 3998 ACCTGC ACCTGC(4/8)
1219 GCAGGT
BsrBI 453 CCGCTC CCGCTC(-3/-3)
2687 GAGCGG
BsrFI 819 GCCGGC [GA]^CCGG[CT]
4067 GCCGGC
BsrGI 2923 TGTACA T^GTACA
3563 TGTACA
BstUI 743 CGCG CG^CG
3935 CGCG
Cfr10I 819 GCCGGC [GA]^CCGG[CT]
4067 GCCGGC
Eco47III 1200 AGCGCT AGC^GCT
2710 AGCGCT
HaeII 1200 AGCGCT [GA]GCGC^[CT]
2710 AGCGCT
MvnI 743 CGCG CG^CG
3935 CGCG
NaeI 819 GCCGGC GCC^GGC
4067 GCCGGC
NcoI 771 CCATGG C^CATGG
805 CCATGG
NgoMIV 819 GCCGGC G^CCGGC
4067 GCCGGC
PshBI 2903 ATTAAT AT^TAAT
2918 ATTAAT
PstI 842 CTGCAG CTGCA^G
4000 CTGCAG
SapI 362 GAAGAGC GCTCTTC(1/4)
737 GCTCTTC
SspBI 2923 TGTACA T^GTACA
3563 TGTACA
XcmI 3209 CCAACACCTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3355 CCAGGCGGGAGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AocI ApoI AspHI AspI
AvaI AvaII BalI BanII BbvCI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflFI PleI PpuMI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I XhoII
XmaCI XmaI