##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AccIII AhdI Alw44I AocI ApaLI
AscI AseI AsnI AspEI AvrII
BbeI BbrPI BbvCI BciVI BclI
BglII BlnI BseAI BsiWI Bsp1407I
BspEI BsrGI BssHII BssSI Bst1107I
BstBI BstZ17I Bsu36I DraI DrdI
EagI Eam1105I EclXI Eco52I Eco81I
EcoRI FbaI FseI HindIII HpaI
KasI KspI MfeI MluI MroI
MunI NarI NdeI NheI NotI
NruI NspV PacI PaeR7I PmaCI
PmeI PmlI PpuMI PshBI SacII
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SspBI StuI SwaI
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 2774 GACGTC GACGT^C
Acc65I 2534 GGTACC G^GTACC
AclI 1862 AACGTT AA^CGTT
AflII 351 CTTAAG C^TTAAG
AgeI 2581 ACCGGT A^CCGGT
Asp700I 1826 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2534 GGTACC G^GTACC
AvaI 2657 CTCGGG C^[CT]CG[GA]G
BamHI 3544 GGATCC G^GATCC
BcgI 2177 CGAGGGCAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 351 CTTAAG C^TTAAG
BlpI 3074 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3074 GCTCAGC GC^T[ACGT]AGC
BsaAI 2400 CACGTA [CT]AC^GT[GA]
BsaBI 165 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 662 CGTCTC CGTCTC(1/5)
BsmI 1784 GCATTC GAATGC(1/-1)
BsoBI 2657 CTCGGG C^[CT]CG[GA]G
BspDI 1956 ATCGAT AT^CGAT
BstAPI 2593 GCAACTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 3544 GGATCC [GA]^GATC[CT]
CelII 3074 GCTCAGC GC^T[ACGT]AGC
ClaI 1956 ATCGAT AT^CGAT
CpoI 2583 CGGTCCG CG^G[AT]CCG
DraIII 2517 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRV 3750 GATATC GAT^ATC
HgaI 3198 GCGTC GACGC(5/10)
HincII 525 GTCGAC GT[CT]^[GA]AC
HindII 525 GTCGAC GT[CT]^[GA]AC
KpnI 2534 GGTACC GGTAC^C
MamI 165 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 3544 GGATCC [GA]^GATC[CT]
PflMI 1574 CCATGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 2581 ACCGGT A^CCGGT
Psp1406I 1862 AACGTT AA^CGTT
RsrII 2583 CGGTCCG CG^G[AT]CCG
SacI 3741 GAGCTC GAGCT^C
SalI 525 GTCGAC G^TCGAC
SbfI 2735 CCTGCAGG CCTGCA^GG
SmlI 351 CTTAAG C^T[CT][GA]AG
Sse8387I 2735 CCTGCAGG CCTGCA^GG
SspI 1038 AATATT AAT^ATT
Van91I 1574 CCATGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 3544 GGATCC [GA]^GATC[CT]
XmnI 1826 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 525 GTCGAC GT^[AC][GT]AC
2403 GTAGAC
AcyI 2774 GACGTC G[GA]^CG[CT]C
3197 GGCGTC
AlwNI 145 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
2739 CAGGAACTG
Aor51HI 621 AGCGCT AGC^GCT
2317 AGCGCT
AspI 2451 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3096 GACCGGGTC
AviII 1663 TGCGCA TGC^GCA
3471 TGCGCA
BalI 886 TGGCCA TGG^CCA
2108 TGGCCA
BbsI 1419 GTCTTC GAAGAC(2/6)
1539 GTCTTC
BfaI 3391 CTAG C^TAG
3935 CTAG
BpuAI 1419 GTCTTC GAAGAC(2/6)
1539 GTCTTC
BsaHI 2774 GACGTC G[GA]^CG[CT]C
3197 GGCGTC
BspLU11I 1426 ACATGT A^CATGT
2971 ACATGT
Eco47III 621 AGCGCT AGC^GCT
2317 AGCGCT
EcoT22I 1062 ATGCAT ATGCA^T
1105 ATGCAT
FspI 1663 TGCGCA TGC^GCA
3471 TGCGCA
Hin1I 2774 GACGTC G[GA]^CG[CT]C
3197 GGCGTC
MaeI 3391 CTAG C^TAG
3935 CTAG
MluNI 886 TGGCCA TGG^CCA
2108 TGGCCA
MscI 886 TGGCCA TGG^CCA
2108 TGGCCA
NaeI 2753 GCCGGC GCC^GGC
2854 GCCGGC
NgoMIV 2753 GCCGGC G^CCGGC
2854 GCCGGC
NsiI 1062 ATGCAT ATGCA^T
1105 ATGCAT
PflFI 2451 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3096 GACCGGGTC
PshAI 1735 GACGGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3926 GACATCTGTC
PvuI 513 CGATCG CGAT^CG
1585 CGATCG
SapI 153 GAAGAGC GCTCTTC(1/4)
3738 GAAGAGC
ScaI 118 AGTACT AGT^ACT
691 AGTACT
SexAI 394 ACCTGGT A^CC[AT]GGT
940 ACCAGGT
SphI 1064 GCATGC GCATG^C
3714 GCATGC
Tth111I 2451 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3096 GACCGGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI ApaI ApoI AspHI
AvaII BanI BanII BbvI BcnI
BglI BmyI BpmI BsaI BsaJI
BsaWI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI
BsrI BssKI BstEII BstF5I BstNI
BstPI BstUI BstXI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI