################################## # NO CUT ENZYMES ( 74 enzymes) # ################################## AccIII AhdI Alw44I AocI ApaLI AscI AseI AsnI AspEI AvrII BbeI BbrPI BbvCI BciVI BclI BglII BlnI BseAI BsiWI Bsp1407I BspEI BsrGI BssHII BssSI Bst1107I BstBI BstZ17I Bsu36I DraI DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoRI FbaI FseI HindIII HpaI KasI KspI MfeI MluI MroI MunI NarI NdeI NheI NotI NruI NspV PacI PaeR7I PmaCI PmeI PmlI PpuMI PshBI SacII SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SspBI StuI SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AatII 2774 GACGTC GACGT^C Acc65I 2534 GGTACC G^GTACC AclI 1862 AACGTT AA^CGTT AflII 351 CTTAAG C^TTAAG AgeI 2581 ACCGGT A^CCGGT Asp700I 1826 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 2534 GGTACC G^GTACC AvaI 2657 CTCGGG C^[CT]CG[GA]G BamHI 3544 GGATCC G^GATCC BcgI 2177 CGAGGGCAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 351 CTTAAG C^TTAAG BlpI 3074 GCTCAGC GC^T[ACGT]AGC Bpu1102I 3074 GCTCAGC GC^T[ACGT]AGC BsaAI 2400 CACGTA [CT]AC^GT[GA] BsaBI 165 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmBI 662 CGTCTC CGTCTC(1/5) BsmI 1784 GCATTC GAATGC(1/-1) BsoBI 2657 CTCGGG C^[CT]CG[GA]G BspDI 1956 ATCGAT AT^CGAT BstAPI 2593 GCAACTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 3544 GGATCC [GA]^GATC[CT] CelII 3074 GCTCAGC GC^T[ACGT]AGC ClaI 1956 ATCGAT AT^CGAT CpoI 2583 CGGTCCG CG^G[AT]CCG DraIII 2517 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG EcoRV 3750 GATATC GAT^ATC HgaI 3198 GCGTC GACGC(5/10) HincII 525 GTCGAC GT[CT]^[GA]AC HindII 525 GTCGAC GT[CT]^[GA]AC KpnI 2534 GGTACC GGTAC^C MamI 165 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 3544 GGATCC [GA]^GATC[CT] PflMI 1574 CCATGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 2581 ACCGGT A^CCGGT Psp1406I 1862 AACGTT AA^CGTT RsrII 2583 CGGTCCG CG^G[AT]CCG SacI 3741 GAGCTC GAGCT^C SalI 525 GTCGAC G^TCGAC SbfI 2735 CCTGCAGG CCTGCA^GG SmlI 351 CTTAAG C^T[CT][GA]AG Sse8387I 2735 CCTGCAGG CCTGCA^GG SspI 1038 AATATT AAT^ATT Van91I 1574 CCATGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 3544 GGATCC [GA]^GATC[CT] XmnI 1826 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccI 525 GTCGAC GT^[AC][GT]AC 2403 GTAGAC AcyI 2774 GACGTC G[GA]^CG[CT]C 3197 GGCGTC AlwNI 145 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG 2739 CAGGAACTG Aor51HI 621 AGCGCT AGC^GCT 2317 AGCGCT AspI 2451 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC 3096 GACCGGGTC AviII 1663 TGCGCA TGC^GCA 3471 TGCGCA BalI 886 TGGCCA TGG^CCA 2108 TGGCCA BbsI 1419 GTCTTC GAAGAC(2/6) 1539 GTCTTC BfaI 3391 CTAG C^TAG 3935 CTAG BpuAI 1419 GTCTTC GAAGAC(2/6) 1539 GTCTTC BsaHI 2774 GACGTC G[GA]^CG[CT]C 3197 GGCGTC BspLU11I 1426 ACATGT A^CATGT 2971 ACATGT Eco47III 621 AGCGCT AGC^GCT 2317 AGCGCT EcoT22I 1062 ATGCAT ATGCA^T 1105 ATGCAT FspI 1663 TGCGCA TGC^GCA 3471 TGCGCA Hin1I 2774 GACGTC G[GA]^CG[CT]C 3197 GGCGTC MaeI 3391 CTAG C^TAG 3935 CTAG MluNI 886 TGGCCA TGG^CCA 2108 TGGCCA MscI 886 TGGCCA TGG^CCA 2108 TGGCCA NaeI 2753 GCCGGC GCC^GGC 2854 GCCGGC NgoMIV 2753 GCCGGC G^CCGGC 2854 GCCGGC NsiI 1062 ATGCAT ATGCA^T 1105 ATGCAT PflFI 2451 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC 3096 GACCGGGTC PshAI 1735 GACGGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 3926 GACATCTGTC PvuI 513 CGATCG CGAT^CG 1585 CGATCG SapI 153 GAAGAGC GCTCTTC(1/4) 3738 GAAGAGC ScaI 118 AGTACT AGT^ACT 691 AGTACT SexAI 394 ACCTGGT A^CC[AT]GGT 940 ACCAGGT SphI 1064 GCATGC GCATG^C 3714 GCATGC Tth111I 2451 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC 3096 GACCGGGTC ######################################### # 3 OR MORE CUT ENZYMES ( 109 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI AlwI ApaI ApoI AspHI AvaII BanI BanII BbvI BcnI BglI BmyI BpmI BsaI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrI BssKI BstEII BstF5I BstNI BstPI BstUI BstXI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI