##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII Acc65I AflII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
Asp718I AspEI AspI AviII AvrII
BbeI BbrPI BcgI BciVI BfrI
BglI BlnI BsiEI BsiWI Bsp1407I
BspDI BsrBI BsrGI BssHII BstAPI
BstEII BstPI Bsu36I ClaI CpoI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRV FseI FspI
HpaI KasI KpnI KspI MfeI
MluI MunI NarI NcoI NheI
NotI NruI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PvuI RsrII
SacII SalI ScaI SexAI SfiI
SfoI SgrAI SnaBI SpeI SphI
SspBI SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AccII 25 CGCG CG^CG
AccIII 1694 TCCGGA T^CCGGA
AclI 1627 AACGTT AA^CGTT
ApaI 2396 GGGCCC GGGCC^C
Asp700I 2109 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 907 GGATCC G^GATCC
BbvCI 3276 CCTCAGC CCTCAGC(-5/-2)
BclI 3007 TGATCA T^GATCA
BglII 2802 AGATCT A^GATCT
BlpI 2499 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 2499 GCTAAGC GC^T[ACGT]AGC
BsaAI 615 CACGTA [CT]AC^GT[GA]
BsaBI 1971 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1694 TCCGGA T^CCGGA
BseRI 1487 GAGGAG GAGGAG(10/8)
BsmBI 1789 GAGACG CGTCTC(1/5)
BspEI 1694 TCCGGA T^CCGGA
BspLU11I 2223 ACATGT A^CATGT
BsrDI 868 GCAATG GCAATG(2/0)
BssSI 219 CACGAG CACGAG(-5/-1)
Bst1107I 3521 GTATAC GTA^TAC
BstBI 587 TTCGAA TT^CGAA
BstUI 25 CGCG CG^CG
BstXI 943 CCATCGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 3521 GTATAC GTA^TAC
CelII 2499 GCTAAGC GC^T[ACGT]AGC
DraIII 2995 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 889 GACGAAGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1986 CCGTGG C^C[GA][CT]GG
EcoNI 231 CCTGTTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 1309 CCCGC CCCGC(4/6)
FbaI 3007 TGATCA T^GATCA
HaeII 47 AGCGCC [GA]GCGC^[CT]
HindIII 3781 AAGCTT A^AGCTT
MamI 1971 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1694 TCCGGA T^CCGGA
MvnI 25 CGCG CG^CG
NdeI 350 CATATG CA^TATG
NspV 587 TTCGAA TT^CGAA
PacI 2513 TTAATTAA TTAAT^TAA
PflMI 743 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 337 GACTTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1627 AACGTT AA^CGTT
PvuII 1867 CAGCTG CAG^CTG
SacI 987 GAGCTC GAGCT^C
SapI 110 GAAGAGC GCTCTTC(1/4)
SbfI 51 CCTGCAGG CCTGCA^GG
SfuI 587 TTCGAA TT^CGAA
SmaI 185 CCCGGG CCC^GGG
SmlI 2945 CTCAAG C^T[CT][GA]AG
Sse8387I 51 CCTGCAGG CCTGCA^GG
SspI 2744 AATATT AAT^ATT
StuI 1222 AGGCCT AGG^CCT
Van91I 743 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2693 TCTAGA T^CTAGA
XmaCI 185 CCCGGG C^CCGGG
XmaI 185 CCCGGG C^CCGGG
XmnI 2109 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 3483 GTAGAC GT^[AC][GT]AC
3521 GTATAC
AcyI 190 GGCGTC G[GA]^CG[CT]C
934 GGCGTC
AflIII 614 ACACGT A^C[GA][CT]GT
2223 ACATGT
AseI 1464 ATTAAT AT^TAAT
3762 ATTAAT
AsnI 1464 ATTAAT AT^TAAT
3762 ATTAAT
BalI 1011 TGGCCA TGG^CCA
1934 TGGCCA
BanI 31 GGCACC G^G[CT][GA]CC
180 GGTGCC
BbsI 2373 GAAGAC GAAGAC(2/6)
519 GTCTTC
BpuAI 2373 GAAGAC GAAGAC(2/6)
519 GTCTTC
BsaHI 190 GGCGTC G[GA]^CG[CT]C
934 GGCGTC
BspHI 482 TCATGA T^CATGA
1610 TCATGA
BsrFI 1849 GCCGGC [GA]^CCGG[CT]
2162 GCCGGC
CfoI 7 GCGC GCG^C
48 GCGC
Cfr10I 1849 GCCGGC [GA]^CCGG[CT]
2162 GCCGGC
EcoT22I 2975 ATGCAT ATGCA^T
3716 ATGCAT
HhaI 7 GCGC GCG^C
48 GCGC
Hin1I 190 GGCGTC G[GA]^CG[CT]C
934 GGCGTC
HinP1I 7 GCGC G^CGC
48 GCGC
MluNI 1011 TGGCCA TGG^CCA
1934 TGGCCA
MscI 1011 TGGCCA TGG^CCA
1934 TGGCCA
MspA1I 140 CAGCGG C[AC]G^C[GT]G
1867 CAGCTG
NaeI 1849 GCCGGC GCC^GGC
2162 GCCGGC
NgoMIV 1849 GCCGGC G^CCGGC
2162 GCCGGC
NsiI 2975 ATGCAT ATGCA^T
3716 ATGCAT
PshBI 1464 ATTAAT AT^TAAT
3762 ATTAAT
RcaI 482 TCATGA T^CATGA
1610 TCATGA
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaI AvaII
BanII BbvI BcnI BfaI BmyI
BpmI BsaI BsaJI BsaWI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraI DraII
EaeI EarI EcoO109I EcoRI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HgaI HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII