##################################
# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI Aor51HI ApaI AscI
Asp700I Asp718I AvaI AviII BamHI
BbeI BbrPI BcnI BfrI BlpI
Bpu1102I BsaBI BsaHI BsaWI BseAI
BseRI BsiWI BsmI BsoBI BspDI
BspEI BsrBI BsrFI BssHII BssSI
BstBI BstEII BstPI CelII Cfr10I
ClaI CpoI DraII DraIII DrdI
EagI EclXI Eco47III Eco52I EcoNI
EcoO109I EcoO65I EcoT22I FseI FspI
HaeII HapII Hin1I HpaII KasI
KpnI KspI MamI MluI MroI
MspI NaeI NarI NciI NgoMIV
NheI NotI NruI NsiI NspV
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI Psp1406I RsrII SacI SacII
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SwaI XbaI XcmI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AhdI 519 GACGTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3599 GTGCAC G^TGCAC
AlwNI 3949 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 3599 GTGCAC G^TGCAC
AspEI 519 GACGTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 3599 GTGCAC G[AT]GC[AT]^C
AspI 1374 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 1164 GGACC G^G[AT]CC
AvrII 2606 CCTAGG C^CTAGG
BanI 1257 GGTGCC G^G[CT][GA]CC
BbvCI 1285 GCTGAGG CCTCAGC(-5/-2)
BciVI 3269 GTATCC GTATCC(6/5)
BclI 1058 TGATCA T^GATCA
BlnI 2606 CCTAGG C^CTAGG
BsaI 1187 GAGACC GGTCTC(1/5)
BsiEI 1446 CGATCG CG[GA][CT]^CG
BsiHKAI 3599 GTGCAC G[AT]GC[AT]^C
BsmBI 1379 CGTCTC CGTCTC(1/5)
Bsp1407I 3011 TGTACA T^GTACA
BspHI 3017 TCATGA T^CATGA
BspMI 1190 ACCTGC ACCTGC(4/8)
BsrGI 3011 TGTACA T^GTACA
Bst1107I 314 GTATAC GTA^TAC
BstAPI 1800 GCAGTTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1128 CCAACACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 314 GTATAC GTA^TAC
Cfr13I 1164 GGACC G^G[ACGT]CC
Eam1105I 519 GACGTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 2397 CTCTTC CTCTTC(1/4)
EcoRI 4043 GAATTC G^AATTC
EcoRV 3898 GATATC GAT^ATC
FbaI 1058 TGATCA T^GATCA
HgaI 3533 GCGTC GACGC(5/10)
HpaI 3544 GTTAAC GTT^AAC
Ksp632I 2397 CTCTTC CTCTTC(1/4)
MfeI 3131 CAATTG C^AATTG
MunI 3131 CAATTG C^AATTG
NcoI 559 CCATGG C^CATGG
NdeI 3105 CATATG CA^TATG
PacI 955 TTAATTAA TTAAT^TAA
PflFI 1374 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1310 CCAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1991 GACACCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 1446 CGATCG CGAT^CG
RcaI 3017 TCATGA T^CATGA
SalI 144 GTCGAC G^TCGAC
SapI 2396 GCTCTTC GCTCTTC(1/4)
Sau96I 1164 GGACC G^G[ACGT]CC
ScaI 1951 AGTACT AGT^ACT
SmlI 2718 CTCAAG C^T[CT][GA]AG
SspBI 3011 TGTACA T^GTACA
StuI 2699 AGGCCT AGG^CCT
Tth111I 1374 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1310 CCAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 144 GTCGAC GT^[AC][GT]AC
314 GTATAC
AccII 1341 CGCG CG^CG
3680 CGCG
AocI 1014 CCTTAGG CC^T[ACGT]AGG
1170 CCTGAGG
AseI 764 ATTAAT AT^TAAT
2374 ATTAAT
AsnI 764 ATTAAT AT^TAAT
2374 ATTAAT
BalI 1206 TGGCCA TGG^CCA
2704 TGGCCA
BcgI 2383 CGAACCGTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1291 GCATAAGAATCG
BglI 1199 GCCAATATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1703 GCCTCTGAGGC
BpmI 1353 CTCCAG CTGGAG(16/14)
4303 CTCCAG
BsaAI 2092 TACGTG [CT]AC^GT[GA]
4334 TACGTG
BsrDI 4202 CATTGC GCAATG(2/0)
3794 GCAATG
BstUI 1341 CGCG CG^CG
3680 CGCG
Bsu36I 1014 CCTTAGG CC^T[ACGT]AGG
1170 CCTGAGG
DsaI 178 CCGTGG C^C[GA][CT]GG
559 CCATGG
Eco81I 1014 CCTTAGG CC^T[ACGT]AGG
1170 CCTGAGG
FauI 708 GCGGG CCCGC(4/6)
1158 GCGGG
HindIII 280 AAGCTT A^AGCTT
3505 AAGCTT
MluNI 1206 TGGCCA TGG^CCA
2704 TGGCCA
MscI 1206 TGGCCA TGG^CCA
2704 TGGCCA
MspA1I 1437 CAGCTG C[AC]G^C[GT]G
3974 CAGCTG
MvnI 1341 CGCG CG^CG
3680 CGCG
PshBI 764 ATTAAT AT^TAAT
2374 ATTAAT
PvuII 1437 CAGCTG CAG^CTG
3974 CAGCTG
SexAI 148 ACCTGGT A^CC[AT]GGT
1467 ACCAGGT
SspI 1554 AATATT AAT^ATT
3490 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI BanII BbsI BbvI
BfaI BglII BmyI BpuAI BsaJI
BsgI BsiYI BslI BsmAI BsmFI
Bsp1286I BspLU11I BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI DdeI
DpnII DraI EaeI EcoRII EcoT14I
Fnu4HI FokI HaeIII HhaI HinP1I
HincII HindII HinfI HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PstI RsaI Sau3AI ScrFI
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII