##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII AclI AgeI Alw44I AocI
ApaLI AscI AseI AsnI AvaI
AviII AvrII BamHI BbvCI BciVI
BlnI BsiEI BsiWI BsmBI BsoBI
BspDI BspHI BspLU11I BssHII Bst1107I
BstAPI BstBI BstZ17I Bsu36I ClaI
CpoI EagI EclXI Eco52I Eco81I
EcoRV FseI FspI KspI MluI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflMI PinAI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI Sse8387I
StuI SwaI Van91I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
Acc65I 1235 GGTACC G^GTACC
AccIII 2184 TCCGGA T^CCGGA
Aor51HI 267 AGCGCT AGC^GCT
ApaI 214 GGGCCC GGGCC^C
Asp718I 1235 GGTACC G^GTACC
AspI 149 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2145 TGGCCA TGG^CCA
BbeI 415 GGCGCC GGCGC^C
BcgI 2561 CGATGTGATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 283 GCCGAAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 3605 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3605 GCTGAGC GC^T[ACGT]AGC
BsaWI 2184 TCCGGA [AT]^CCGG[AT]
BseAI 2184 TCCGGA T^CCGGA
BsgI 2317 GTGCAG GTGCAG(16/14)
BspEI 2184 TCCGGA T^CCGGA
BsrBI 3458 CCGCTC CCGCTC(-3/-3)
BsrDI 3662 CATTGC GCAATG(2/0)
BsrFI 103 GCCGGT [GA]^CCGG[CT]
BssSI 1041 CACGAG CACGAG(-5/-1)
BstEII 4021 GGTCACC G^GT[ACGT]ACC
BstPI 4021 GGTCACC G^GT[ACGT]ACC
BstXI 3769 CCAATGGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 3605 GCTGAGC GC^T[ACGT]AGC
Cfr10I 103 GCCGGT [GA]^CCGG[CT]
Eco47III 267 AGCGCT AGC^GCT
EcoO65I 4021 GGTCACC G^GT[ACGT]ACC
EcoRI 1841 GAATTC G^AATTC
EcoT22I 3104 ATGCAT ATGCA^T
HgaI 3548 GCGTC GACGC(5/10)
HindIII 1684 AAGCTT A^AGCTT
KasI 415 GGCGCC G^GCGCC
KpnI 1235 GGTACC GGTAC^C
MluNI 2145 TGGCCA TGG^CCA
MroI 2184 TCCGGA T^CCGGA
MscI 2145 TGGCCA TGG^CCA
MspA1I 32 CAGCGG C[AC]G^C[GT]G
NarI 415 GGCGCC GG^CGCC
NsiI 3104 ATGCAT ATGCA^T
PflFI 149 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmeI 560 GTTTAAAC GTTT^AAAC
PpuMI 3384 AGGTCCC [GA]G^G[AT]CC[CT]
PstI 2651 CTGCAG CTGCA^G
SfoI 415 GGCGCC GGC^GCC
SnaBI 572 TACGTA TAC^GTA
SpeI 1440 ACTAGT A^CTAGT
SphI 3106 GCATGC GCATG^C
Tth111I 149 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3405 TCTAGA T^CTAGA
XcmI 1677 CCAAAGCAAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AccI 3505 GTAGAC GT^[AC][GT]AC
3536 GTCTAC
AcyI 415 GGCGCC G[GA]^CG[CT]C
3547 GGCGTC
AhdI 3121 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3687 GACCAGCAGTC
AlwNI 1905 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
2611 CAGCATCTG
Asp700I 1576 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4105 GAAGACTTTC
AspEI 3121 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3687 GACCAGCAGTC
BanI 415 GGCGCC G^G[CT][GA]CC
1235 GGTACC
BbrPI 1299 CACGTG CAC^GTG
1662 CACGTG
BbsI 4105 GAAGAC GAAGAC(2/6)
1535 GTCTTC
BclI 1132 TGATCA T^GATCA
1978 TGATCA
BcnI 2881 CCCGG CC^[GC]GG
3439 CCCGG
BpmI 1786 CTCCAG CTGGAG(16/14)
1757 CTGGAG
BpuAI 4105 GAAGAC GAAGAC(2/6)
1535 GTCTTC
BsaHI 415 GGCGCC G[GA]^CG[CT]C
3547 GGCGTC
Bsp1407I 3613 TGTACA T^GTACA
4372 TGTACA
BspMI 4119 ACCTGC ACCTGC(4/8)
3481 GCAGGT
BsrGI 3613 TGTACA T^GTACA
4372 TGTACA
DraII 170 GGGGCCT [GA]G^G[ACGT]CC[CT]
3384 AGGTCCC
DraIII 460 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
2389 CACGCAGTG
DrdI 333 GACCACAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1030 GACTCCAATGTC
DsaI 139 CCACGG C^C[GA][CT]GG
374 CCACGG
EaeI 1547 TGGCCG [CT]^GGCC[GA]
2145 TGGCCA
Eam1105I 3121 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3687 GACCAGCAGTC
EcoNI 503 CCTCTTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2076 CCTCTCAAAGG
EcoO109I 170 GGGGCCT [GA]G^G[ACGT]CC[CT]
3384 AGGTCCC
FbaI 1132 TGATCA T^GATCA
1978 TGATCA
HaeII 267 AGCGCT [GA]GCGC^[CT]
415 GGCGCC
Hin1I 415 GGCGCC G[GA]^CG[CT]C
3547 GGCGTC
HpaI 115 GTTAAC GTT^AAC
1490 GTTAAC
MfeI 1368 CAATTG C^AATTG
1622 CAATTG
MslI 1730 CATGCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1996 CATTTGGGTG
MunI 1368 CAATTG C^AATTG
1622 CAATTG
NciI 2881 CCCGG CC^[GC]GG
3439 CCCGG
PmaCI 1299 CACGTG CAC^GTG
1662 CACGTG
PmlI 1299 CACGTG CAC^GTG
1662 CACGTG
SacI 1100 GAGCTC GAGCT^C
1282 GAGCTC
SspBI 3613 TGTACA T^GTACA
4372 TGTACA
XmnI 1576 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4105 GAAGACTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AccII AciI AcsI AfaI AflII
AflIII AluI AlwI ApoI AspHI
AvaII BanII BbvI BfaI BfrI
BglII BmyI BsaAI BsaBI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI EarI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MflI
MnlI MseI MspI MvaI MvnI
MwoI NdeII NlaIII NlaIV NspI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII