##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Aor51HI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BalI BanI BbeI BbrPI
BbvCI BcgI BcnI BfrI BglI
BlnI BsaWI BseAI BsiEI BsiWI
BspDI BspEI BsrFI BssHII BstBI
BstEII BstPI Cfr10I ClaI CpoI
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRV FseI FspI HapII HpaI
HpaII KasI KpnI KspI MluI
MluNI MroI MscI MspI NaeI
NarI NciI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SbfI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I Tth111I Van91I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccI 3170 GTATAC GT^[AC][GT]AC
AcyI 2119 GACGCC G[GA]^CG[CT]C
Alw44I 2037 GTGCAC G^TGCAC
AocI 449 CCTAAGG CC^T[ACGT]AGG
ApaI 1672 GGGCCC GGGCC^C
ApaLI 2037 GTGCAC G^TGCAC
AvaI 7 CTCGGG C^[CT]CG[GA]G
BamHI 99 GGATCC G^GATCC
BanII 1672 GGGCCC G[GA]GC[CT]^C
BglII 1343 AGATCT A^GATCT
BlpI 2608 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2608 GCTCAGC GC^T[ACGT]AGC
BsaHI 2119 GACGCC G[GA]^CG[CT]C
BsgI 1953 CTGCAC GTGCAG(16/14)
BsmBI 2117 GAGACG CGTCTC(1/5)
BsoBI 7 CTCGGG C^[CT]CG[GA]G
BspMI 400 ACCTGC ACCTGC(4/8)
BsrBI 4 CCGCTC CCGCTC(-3/-3)
Bst1107I 3170 GTATAC GTA^TAC
BstAPI 3643 GCAGAACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 3170 GTATAC GTA^TAC
Bsu36I 449 CCTAAGG CC^T[ACGT]AGG
CelII 2608 GCTCAGC GC^T[ACGT]AGC
DsaI 1192 CCATGG C^C[GA][CT]GG
Eco81I 449 CCTAAGG CC^T[ACGT]AGG
EcoT22I 2277 ATGCAT ATGCA^T
FauI 1066 GCGGG CCCGC(4/6)
HaeII 2260 GGCGCT [GA]GCGC^[CT]
Hin1I 2119 GACGCC G[GA]^CG[CT]C
HincII 3487 GTCAAC GT[CT]^[GA]AC
HindII 3487 GTCAAC GT[CT]^[GA]AC
HindIII 2671 AAGCTT A^AGCTT
MfeI 1892 CAATTG C^AATTG
MunI 1892 CAATTG C^AATTG
NcoI 1192 CCATGG C^CATGG
NsiI 2277 ATGCAT ATGCA^T
SexAI 606 ACCAGGT A^CC[AT]GGT
SphI 2613 GCATGC GCATG^C
SwaI 3593 ATTTAAAT ATTT^AAAT
XcmI 2750 CCAGACTAATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AspHI 2037 GTGCAC G[AT]GC[AT]^C
3119 GTGCTC
BclI 3052 TGATCA T^GATCA
3366 TGATCA
BsaBI 762 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2274 GATATGCATC
BsaI 1883 GAGACC GGTCTC(1/5)
610 GGTCTC
BseRI 3602 CTCCTC GAGGAG(10/8)
1311 GAGGAG
BsiHKAI 2037 GTGCAC G[AT]GC[AT]^C
3119 GTGCTC
BsmFI 11 GGGAC GGGAC(10/14)
413 GGGAC
BspLU11I 2765 ACATGT A^CATGT
2800 ACATGT
BssSI 1424 CTCGTG CACGAG(-5/-1)
1928 CTCGTG
DraII 67 AGGCCCT [GA]G^G[ACGT]CC[CT]
1672 GGGCCCC
EcoO109I 67 AGGCCCT [GA]G^G[ACGT]CC[CT]
1672 GGGCCCC
EcoT14I 1192 CCATGG C^C[AT][AT]GG
3394 CCTTGG
FbaI 3052 TGATCA T^GATCA
3366 TGATCA
HgaI 2119 GACGC GACGC(5/10)
1615 GCGTC
MamI 762 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2274 GATATGCATC
MspA1I 151 CAGCTG C[AC]G^C[GT]G
2087 CAGCTG
NdeI 1359 CATATG CA^TATG
2896 CATATG
PstI 24 CTGCAG CTGCA^G
168 CTGCAG
PvuII 151 CAGCTG CAG^CTG
2087 CAGCTG
ScaI 2862 AGTACT AGT^ACT
2992 AGTACT
SfcI 24 CTGCAG C^T[GA][CT]AG
168 CTGCAG
SmlI 613 CTCAAG C^T[CT][GA]AG
2348 CTCAAG
StyI 1192 CCATGG C^C[AT][AT]GG
3394 CCTTGG
TaqI 371 TCGA T^CGA
2357 TCGA
TthHB8I 371 TCGA T^CGA
2357 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI Asp700I
AvaII BbsI BbvI BciVI BfaI
BmyI BpmI BpuAI BsaAI BsaJI
BsiYI BslI BsmAI BsmI Bsp1286I
Bsp1407I BspHI BsrDI BsrGI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI EarI EcoRI EcoRII
Fnu4HI FokI HaeIII HhaI HinP1I
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MvaI MvnI
MwoI NdeII NlaIII NlaIV NspI
PleI RcaI RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SspBI SspI
StuI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII XmnI