##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AgeI Aor51HI ApoI AscI AseI
AsnI Asp700I Asp718I AviII AvrII
BciVI BclI BfrI BglII BlnI
BsiWI BsmI BspDI Bst1107I BstBI
BstZ17I ClaI Eco47III EcoRI EcoRV
EcoT22I FbaI FspI HindIII KpnI
MfeI MluI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I PvuI SalI SapI SbfI
ScaI SexAI SfiI SfuI SnaBI
SpeI Sse8387I SspI SwaI XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 25 enzymes) #
###################################
AccI 2486 GTCTAC GT^[AC][GT]AC
AccIII 500 TCCGGA T^CCGGA
BsaBI 1309 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 500 TCCGGA T^CCGGA
Bsp1407I 1356 TGTACA T^GTACA
BspEI 500 TCCGGA T^CCGGA
BspHI 701 TCATGA T^CATGA
BspLU11I 749 ACATGT A^CATGT
BsrDI 791 GCAATG GCAATG(2/0)
BsrGI 1356 TGTACA T^GTACA
BssHII 4252 GCGCGC G^CGCGC
BstAPI 4243 GCACACACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CpoI 545 CGGACCG CG^G[AT]CCG
DraI 4376 TTTAAA TTT^AAA
DrdI 283 GACCCTGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FseI 3316 GGCCGGCC GGCCGG^CC
HpaI 4339 GTTAAC GTT^AAC
KspI 2535 CCGCGG CCGC^GG
MamI 1309 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 500 TCCGGA T^CCGGA
RcaI 701 TCATGA T^CATGA
RsrII 545 CGGACCG CG^G[AT]CCG
SacI 3604 GAGCTC GAGCT^C
SacII 2535 CCGCGG CCGC^GG
SspBI 1356 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AhdI 379 GACAGCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3435 GACCCCCTGTC
AocI 2314 CCTGAGG CC^T[ACGT]AGG
3569 CCTGAGG
AspEI 379 GACAGCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3435 GACCCCCTGTC
AspI 2173 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3986 GACCCTGTC
BsaWI 500 TCCGGA [AT]^CCGG[AT]
4083 ACCGGA
BsmBI 1973 CGTCTC CGTCTC(1/5)
674 GAGACG
BsrBI 1382 GAGCGG CCGCTC(-3/-3)
2652 GAGCGG
BstEII 3088 GGTCACC G^GT[ACGT]ACC
3983 GGTGACC
BstPI 3088 GGTCACC G^GT[ACGT]ACC
3983 GGTGACC
BstXI 2034 CCAGGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2781 CCAGGGTCCTGG
Bsu36I 2314 CCTGAGG CC^T[ACGT]AGG
3569 CCTGAGG
DraIII 902 CACGGGGTG CAC[ACGT][ACGT][ACGT]^GTG
3279 CACCGAGTG
EagI 729 CGGCCG C^GGCCG
3315 CGGCCG
Eam1105I 379 GACAGCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3435 GACCCCCTGTC
EarI 1208 GAAGAG CTCTTC(1/4)
1228 GAAGAG
EclXI 729 CGGCCG C^GGCCG
3315 CGGCCG
Eco52I 729 CGGCCG C^GGCCG
3315 CGGCCG
Eco81I 2314 CCTGAGG CC^T[ACGT]AGG
3569 CCTGAGG
EcoNI 534 CCTTCGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3565 CCTGCCTGAGG
EcoO65I 3088 GGTCACC G^GT[ACGT]ACC
3983 GGTGACC
HincII 1445 GTCAAC GT[CT]^[GA]AC
4339 GTTAAC
HindII 1445 GTCAAC GT[CT]^[GA]AC
4339 GTTAAC
Ksp632I 1208 GAAGAG CTCTTC(1/4)
1228 GAAGAG
PflFI 2173 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3986 GACCCTGTC
SgrAI 33 CACCGGCG C[GA]^CCGG[CT]G
514 CGCCGGCG
TfiI 2822 GAATC G^A[AT]TC
3263 GAATC
Tth111I 2173 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3986 GACCCTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 139 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI Alw44I AlwI AlwNI ApaI
ApaLI AspHI AvaI AvaII BalI
BamHI BanI BanII BbeI BbrPI
BbsI BbvCI BbvI BcgI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BssSI BstF5I BstNI BstUI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NspI PflMI PleI
PmaCI PmlI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI SmlI SphI
StuI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI