##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII AccIII AclI AgeI AhdI
Aor51HI ApaI AscI AspEI AviII
BamHI BbrPI BbvCI BcgI BclI
BglII BlpI Bpu1102I BsaAI BsaWI
BseAI BsiEI BsiWI BsmBI BspDI
BspEI BspMI BsrDI BsrFI BssHII
BssSI BstBI CelII Cfr10I ClaI
CpoI DraIII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoNI
EcoRI EcoT22I FbaI FseI FspI
HindIII Ksp632I KspI MluI MroI
NaeI NgoMIV NheI NotI NruI
NsiI NspV PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacII SalI SapI SbfI SexAI
SfuI SgrAI SnaBI SpeI Sse8387I
StuI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
Acc65I 2892 GGTACC G^GTACC
AccII 213 CGCG CG^CG
AcyI 205 GGCGCC G[GA]^CG[CT]C
Alw44I 1967 GTGCAC G^TGCAC
AlwNI 4690 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 4414 CCTGAGG CC^T[ACGT]AGG
ApaLI 1967 GTGCAC G^TGCAC
Asp700I 3773 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2892 GGTACC G^GTACC
AspI 1396 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 15 CCTAGG C^CTAGG
BbeI 205 GGCGCC GGCGC^C
BciVI 348 GGATAC GTATCC(6/5)
BlnI 15 CCTAGG C^CTAGG
BsaBI 2257 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 205 GGCGCC G[GA]^CG[CT]C
BsrBI 788 CCGCTC CCGCTC(-3/-3)
BstEII 262 GGTAACC G^GT[ACGT]ACC
BstPI 262 GGTAACC G^GT[ACGT]ACC
BstUI 213 CGCG CG^CG
Bsu36I 4414 CCTGAGG CC^T[ACGT]AGG
CfoI 206 GCGC GCG^C
Eco81I 4414 CCTGAGG CC^T[ACGT]AGG
EcoO65I 262 GGTAACC G^GT[ACGT]ACC
EcoRV 1260 GATATC GAT^ATC
HaeII 205 GGCGCC [GA]GCGC^[CT]
HgaI 1051 GCGTC GACGC(5/10)
HhaI 206 GCGC GCG^C
Hin1I 205 GGCGCC G[GA]^CG[CT]C
HinP1I 206 GCGC G^CGC
HpaI 3428 GTTAAC GTT^AAC
KasI 205 GGCGCC G^GCGCC
KpnI 2892 GGTACC GGTAC^C
MaeII 257 ACGT A^CGT
MamI 2257 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1155 CAATTG C^AATTG
MunI 1155 CAATTG C^AATTG
MvnI 213 CGCG CG^CG
NarI 205 GGCGCC GG^CGCC
PacI 4201 TTAATTAA TTAAT^TAA
PaeR7I 3280 CTCGAG C^TCGAG
PflFI 1396 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1424 CCAAATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ScaI 3746 AGTACT AGT^ACT
SfiI 1277 GGCCAGTGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 205 GGCGCC GGC^GCC
SmaI 3206 CCCGGG CCC^GGG
SphI 3448 GCATGC GCATG^C
SwaI 3622 ATTTAAAT ATTT^AAAT
Tth111I 1396 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1424 CCAAATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1939 TCTAGA T^CTAGA
XhoI 3280 CTCGAG C^TCGAG
XmaCI 3206 CCCGGG C^CCGGG
XmaI 3206 CCCGGG C^CCGGG
XmnI 3773 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccI 1007 GTATAC GT^[AC][GT]AC
3097 GTATAC
AflII 3183 CTTAAG C^TTAAG
4559 CTTAAG
AflIII 1891 ACATGT A^C[GA][CT]GT
2389 ACATGT
BfrI 3183 CTTAAG C^TTAAG
4559 CTTAAG
BglI 1278 GCCAGTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1444 GCCTCAAGGGC
BsaI 3234 GGTCTC GGTCTC(1/5)
4221 GGTCTC
BseRI 97 GAGGAG GAGGAG(10/8)
100 GAGGAG
BspLU11I 1891 ACATGT A^CATGT
2389 ACATGT
Bst1107I 1007 GTATAC GTA^TAC
3097 GTATAC
BstYI 1755 AGATCC [GA]^GATC[CT]
4042 GGATCT
BstZ17I 1007 GTATAC GTA^TAC
3097 GTATAC
DraII 3089 AGGTCCC [GA]G^G[ACGT]CC[CT]
3539 AGGACCC
DsaI 227 CCATGG C^C[GA][CT]GG
1412 CCATGG
EcoO109I 3089 AGGTCCC [GA]G^G[ACGT]CC[CT]
3539 AGGACCC
MflI 1755 AGATCC [GA]^GATC[CT]
4042 GGATCT
NcoI 227 CCATGG C^CATGG
1412 CCATGG
PpuMI 3089 AGGTCCC [GA]G^G[AT]CC[CT]
3539 AGGACCC
SacI 3139 GAGCTC GAGCT^C
3300 GAGCTC
XhoII 1755 AGATCC [GA]^GATC[CT]
4042 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AseI AsnI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BspHI BsrGI BsrI
BssKI BstAPI BstF5I BstNI BstXI
Cac8I Cfr13I DdeI DpnII DraI
EaeI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HincII HindII
HinfI HpaII HphI ItaI MaeI
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeI NdeII
NlaIII NlaIV NspI PleI PshBI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI