################################## # NO CUT ENZYMES ( 81 enzymes) # ################################## AatII AccIII AclI AgeI AhdI Aor51HI ApaI AscI AspEI AviII BamHI BbrPI BbvCI BcgI BclI BglII BlpI Bpu1102I BsaAI BsaWI BseAI BsiEI BsiWI BsmBI BspDI BspEI BspMI BsrDI BsrFI BssHII BssSI BstBI CelII Cfr10I ClaI CpoI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoRI EcoT22I FbaI FseI FspI HindIII Ksp632I KspI MluI MroI NaeI NgoMIV NheI NotI NruI NsiI NspV PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacII SalI SapI SbfI SexAI SfuI SgrAI SnaBI SpeI Sse8387I StuI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### Acc65I 2892 GGTACC G^GTACC AccII 213 CGCG CG^CG AcyI 205 GGCGCC G[GA]^CG[CT]C Alw44I 1967 GTGCAC G^TGCAC AlwNI 4690 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG AocI 4414 CCTGAGG CC^T[ACGT]AGG ApaLI 1967 GTGCAC G^TGCAC Asp700I 3773 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 2892 GGTACC G^GTACC AspI 1396 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 15 CCTAGG C^CTAGG BbeI 205 GGCGCC GGCGC^C BciVI 348 GGATAC GTATCC(6/5) BlnI 15 CCTAGG C^CTAGG BsaBI 2257 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 205 GGCGCC G[GA]^CG[CT]C BsrBI 788 CCGCTC CCGCTC(-3/-3) BstEII 262 GGTAACC G^GT[ACGT]ACC BstPI 262 GGTAACC G^GT[ACGT]ACC BstUI 213 CGCG CG^CG Bsu36I 4414 CCTGAGG CC^T[ACGT]AGG CfoI 206 GCGC GCG^C Eco81I 4414 CCTGAGG CC^T[ACGT]AGG EcoO65I 262 GGTAACC G^GT[ACGT]ACC EcoRV 1260 GATATC GAT^ATC HaeII 205 GGCGCC [GA]GCGC^[CT] HgaI 1051 GCGTC GACGC(5/10) HhaI 206 GCGC GCG^C Hin1I 205 GGCGCC G[GA]^CG[CT]C HinP1I 206 GCGC G^CGC HpaI 3428 GTTAAC GTT^AAC KasI 205 GGCGCC G^GCGCC KpnI 2892 GGTACC GGTAC^C MaeII 257 ACGT A^CGT MamI 2257 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1155 CAATTG C^AATTG MunI 1155 CAATTG C^AATTG MvnI 213 CGCG CG^CG NarI 205 GGCGCC GG^CGCC PacI 4201 TTAATTAA TTAAT^TAA PaeR7I 3280 CTCGAG C^TCGAG PflFI 1396 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1424 CCAAATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ScaI 3746 AGTACT AGT^ACT SfiI 1277 GGCCAGTGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 205 GGCGCC GGC^GCC SmaI 3206 CCCGGG CCC^GGG SphI 3448 GCATGC GCATG^C SwaI 3622 ATTTAAAT ATTT^AAAT Tth111I 1396 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1424 CCAAATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 1939 TCTAGA T^CTAGA XhoI 3280 CTCGAG C^TCGAG XmaCI 3206 CCCGGG C^CCGGG XmaI 3206 CCCGGG C^CCGGG XmnI 3773 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AccI 1007 GTATAC GT^[AC][GT]AC 3097 GTATAC AflII 3183 CTTAAG C^TTAAG 4559 CTTAAG AflIII 1891 ACATGT A^C[GA][CT]GT 2389 ACATGT BfrI 3183 CTTAAG C^TTAAG 4559 CTTAAG BglI 1278 GCCAGTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1444 GCCTCAAGGGC BsaI 3234 GGTCTC GGTCTC(1/5) 4221 GGTCTC BseRI 97 GAGGAG GAGGAG(10/8) 100 GAGGAG BspLU11I 1891 ACATGT A^CATGT 2389 ACATGT Bst1107I 1007 GTATAC GTA^TAC 3097 GTATAC BstYI 1755 AGATCC [GA]^GATC[CT] 4042 GGATCT BstZ17I 1007 GTATAC GTA^TAC 3097 GTATAC DraII 3089 AGGTCCC [GA]G^G[ACGT]CC[CT] 3539 AGGACCC DsaI 227 CCATGG C^C[GA][CT]GG 1412 CCATGG EcoO109I 3089 AGGTCCC [GA]G^G[ACGT]CC[CT] 3539 AGGACCC MflI 1755 AGATCC [GA]^GATC[CT] 4042 GGATCT NcoI 227 CCATGG C^CATGG 1412 CCATGG PpuMI 3089 AGGTCCC [GA]G^G[AT]CC[CT] 3539 AGGACCC SacI 3139 GAGCTC GAGCT^C 3300 GAGCTC XhoII 1755 AGATCC [GA]^GATC[CT] 4042 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 102 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AseI AsnI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspHI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstXI Cac8I Cfr13I DdeI DpnII DraI EaeI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeI NdeII NlaIII NlaIV NspI PleI PshBI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI