##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
Acc65I AclI AflII AgeI AhdI
AocI Aor51HI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BbrPI BbvCI BfrI BlnI BlpI
Bpu1102I BsaAI BsiWI BsmI Bsp1407I
BspDI BspHI BsrGI BssSI Bst1107I
BstAPI BstBI BstZ17I Bsu36I CelII
ClaI DrdI Eam1105I Eco47III Eco81I
EcoNI EcoRI EcoT22I FseI FspI
HincII HindII HindIII HpaI KpnI
MfeI MluI MunI NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI SalI
SapI SbfI ScaI SexAI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 26 enzymes) #
###################################
AatII 3354 GACGTC GACGT^C
AccI 2376 GTCTAC GT^[AC][GT]AC
AcsI 1569 AAATTT [GA]^AATT[CT]
Alw44I 1167 GTGCAC G^TGCAC
ApaLI 1167 GTGCAC G^TGCAC
ApoI 1569 AAATTT [GA]^AATT[CT]
Asp700I 632 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BciVI 2119 GTATCC GTATCC(6/5)
BclI 1768 TGATCA T^GATCA
BglII 1717 AGATCT A^GATCT
BsaBI 2865 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 1095 GAGGAG GAGGAG(10/8)
CpoI 810 CGGACCG CG^G[AT]CCG
DraI 2849 TTTAAA TTT^AAA
DraIII 605 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG
FbaI 1768 TGATCA T^GATCA
MamI 2865 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 2244 CATATG CA^TATG
PaeR7I 3298 CTCGAG C^TCGAG
PpuMI 1956 AGGACCT [GA]G^G[AT]CC[CT]
RsrII 810 CGGACCG CG^G[AT]CCG
SfiI 245 GGCCATGCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StuI 3124 AGGCCT AGG^CCT
XcmI 3007 CCATCGGAGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 3298 CTCGAG C^TCGAG
XmnI 632 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccIII 1198 TCCGGA T^CCGGA
1540 TCCGGA
AflIII 1102 ACATGT A^C[GA][CT]GT
3074 ACATGT
BbsI 212 GAAGAC GAAGAC(2/6)
3357 GTCTTC
BcgI 60 GCACTCCGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2356 GCAAGTACATCG
BfaI 158 CTAG C^TAG
777 CTAG
BpuAI 212 GAAGAC GAAGAC(2/6)
3357 GTCTTC
BseAI 1198 TCCGGA T^CCGGA
1540 TCCGGA
BsgI 744 GTGCAG GTGCAG(16/14)
2445 GTGCAG
BsmBI 11 GAGACG CGTCTC(1/5)
165 GAGACG
BspEI 1198 TCCGGA T^CCGGA
1540 TCCGGA
BspLU11I 1102 ACATGT A^CATGT
3074 ACATGT
BspMI 589 ACCTGC ACCTGC(4/8)
1163 GCAGGT
BsrDI 193 CATTGC GCAATG(2/0)
713 CATTGC
BstEII 2618 GGTCACC G^GT[ACGT]ACC
2723 GGTCACC
BstPI 2618 GGTCACC G^GT[ACGT]ACC
2723 GGTCACC
EagI 132 CGGCCG C^GGCCG
432 CGGCCG
EclXI 132 CGGCCG C^GGCCG
432 CGGCCG
Eco52I 132 CGGCCG C^GGCCG
432 CGGCCG
EcoO65I 2618 GGTCACC G^GT[ACGT]ACC
2723 GGTCACC
EcoRV 2202 GATATC GAT^ATC
3392 GATATC
MaeI 158 CTAG C^TAG
777 CTAG
MroI 1198 TCCGGA T^CCGGA
1540 TCCGGA
SmlI 858 CTCAAG C^T[CT][GA]AG
3298 CTCGAG
SphI 1418 GCATGC GCATG^C
2098 GCATGC
Tsp509I 1570 AATT ^AATT
1579 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BamHI BanI BanII
BbeI BbvI BcnI BglI BmyI
BpmI BsaHI BsaI BsaJI BsaWI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrBI BsrFI
BsrI BssHII BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I KspI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PstI
PvuII RsaI SacI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI StyI TaqI TfiI Tru9I
TseI Tsp45I TspRI TthHB8I Van91I
XhoII XmaCI XmaI