##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AgeI AhdI Alw44I Aor51HI ApaLI
ApoI AseI AsnI Asp718I AspEI
AviII AvrII BalI BbrPI BclI
BfrI BlnI BsiEI BsmBI BsmI
Bsp1407I BspDI BspLU11I BsrDI BsrGI
BstBI BstEII BstPI ClaI CpoI
DraI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HindIII HpaI
KpnI MfeI MluI MluNI MscI
MseI MunI NcoI NdeI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SalI SapI
ScaI SfuI SgrAI SmaI SnaBI
SpeI SspBI SwaI Tru9I XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AccIII 2267 TCCGGA T^CCGGA
AscI 1831 GGCGCGCC GG^CGCGCC
AspI 1923 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 3319 CCTCAGC CCTCAGC(-5/-2)
BciVI 2981 GTATCC GTATCC(6/5)
BlpI 2282 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2282 GCTCAGC GC^T[ACGT]AGC
BsaAI 1586 CACGTA [CT]AC^GT[GA]
BsaWI 2267 TCCGGA [AT]^CCGG[AT]
BseAI 2267 TCCGGA T^CCGGA
BsiWI 1588 CGTACG C^GTACG
BspEI 2267 TCCGGA T^CCGGA
BspHI 1615 TCATGA T^CATGA
Bst1107I 1316 GTATAC GTA^TAC
BstZ17I 1316 GTATAC GTA^TAC
CelII 2282 GCTCAGC GC^T[ACGT]AGC
DraIII 810 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1680 GACAGCATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HincII 2391 GTCAAC GT[CT]^[GA]AC
HindII 2391 GTCAAC GT[CT]^[GA]AC
MroI 2267 TCCGGA T^CCGGA
NheI 1890 GCTAGC G^CTAGC
PflFI 1923 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 3339 GACAAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1615 TCATGA T^CATGA
SbfI 1628 CCTGCAGG CCTGCA^GG
SexAI 697 ACCTGGT A^CC[AT]GGT
SfiI 878 GGCCATGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 109 GCATGC GCATG^C
Sse8387I 1628 CCTGCAGG CCTGCA^GG
SspI 3676 AATATT AAT^ATT
TfiI 571 GAATC G^A[AT]TC
Tsp509I 914 AATT ^AATT
Tth111I 1923 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 1316 GTATAC GT^[AC][GT]AC
3494 GTAGAC
AflIII 1585 ACACGT A^C[GA][CT]GT
3533 ACGTGT
Asp700I 200 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1445 GAAGAGGTTC
BamHI 784 GGATCC G^GATCC
3372 GGATCC
BcgI 1553 CGAGGACTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2189 CGAGGACAGTGC
BglII 790 AGATCT A^GATCT
3614 AGATCT
BsaBI 785 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1475 GATCAGCATC
BsrFI 875 GCCGGC [GA]^CCGG[CT]
1986 GCCGGC
BssHII 834 GCGCGC G^CGCGC
1832 GCGCGC
BssSI 319 CACGAG CACGAG(-5/-1)
252 CTCGTG
Cfr10I 875 GCCGGC [GA]^CCGG[CT]
1986 GCCGGC
EaeI 877 CGGCCA [CT]^GGCC[GA]
1723 TGGCCG
KspI 744 CCGCGG CCGC^GG
1648 CCGCGG
MamI 785 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1475 GATCAGCATC
NaeI 875 GCCGGC GCC^GGC
1986 GCCGGC
NgoMIV 875 GCCGGC G^CCGGC
1986 GCCGGC
PvuII 150 CAGCTG CAG^CTG
3565 CAGCTG
SacI 378 GAGCTC GAGCT^C
735 GAGCTC
SacII 744 CCGCGG CCGC^GG
1648 CCGCGG
SmlI 435 CTCAAG C^T[CT][GA]AG
1494 CTCAAG
XmnI 200 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1445 GAAGAGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BanI BanII BbeI
BbsI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
StuI StyI TaqI TseI Tsp45I
TspRI TthHB8I Van91I XcmI XhoII