##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AgeI Alw44I Aor51HI
ApaLI AscI Asp718I AviII AvrII
BalI BbeI BbrPI BbvCI BcgI
BfrI BglI BglII BlnI BsaAI
BsaBI BsaI BsaWI BseAI BsiEI
BsiWI BspEI BsrBI BssHII Bst1107I
BstAPI BstBI BstUI BstXI BstZ17I
CpoI DraIII DrdI EaeI EagI
EclXI Eco47III Eco52I FseI FspI
HincII HindII HpaI KasI KpnI
KspI MamI MluI MluNI MroI
MscI MvnI NarI NheI NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RsrII SacII SalI SbfI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I StuI SwaI
XbaI XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AcyI 940 GGCGTC G[GA]^CG[CT]C
AhdI 2840 GACTAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1959 CCTGAGG CC^T[ACGT]AGG
ApaI 1627 GGGCCC GGGCC^C
AspEI 2840 GACTAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1209 GAGCTC G[AT]GC[AT]^C
AspI 4270 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 625 CCCGAG C^[CT]CG[GA]G
BcnI 2648 CCGGG CC^[GC]GG
BlpI 1206 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1206 GCTGAGC GC^T[ACGT]AGC
BsaHI 940 GGCGTC G[GA]^CG[CT]C
BsiHKAI 1209 GAGCTC G[AT]GC[AT]^C
BsmBI 942 CGTCTC CGTCTC(1/5)
BsoBI 625 CCCGAG C^[CT]CG[GA]G
Bsp1407I 3400 TGTACA T^GTACA
BspDI 1968 ATCGAT AT^CGAT
BsrGI 3400 TGTACA T^GTACA
BssSI 916 CACGAG CACGAG(-5/-1)
Bsu36I 1959 CCTGAGG CC^T[ACGT]AGG
CelII 1206 GCTGAGC GC^T[ACGT]AGC
ClaI 1968 ATCGAT AT^CGAT
Eam1105I 2840 GACTAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1959 CCTGAGG CC^T[ACGT]AGG
EcoNI 1315 CCTCCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 911 GAATTC G^AATTC
EcoRV 4501 GATATC GAT^ATC
FauI 1589 CCCGC CCCGC(4/6)
Hin1I 940 GGCGTC G[GA]^CG[CT]C
NaeI 2664 GCCGGC GCC^GGC
NciI 2648 CCGGG CC^[GC]GG
NgoMIV 2664 GCCGGC G^CCGGC
PflFI 4270 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 4131 GGGACCC [GA]G^G[AT]CC[CT]
PstI 2401 CTGCAG CTGCA^G
SacI 1209 GAGCTC GAGCT^C
SexAI 1611 ACCTGGT A^CC[AT]GGT
SmlI 2431 CTCAAG C^T[CT][GA]AG
SspBI 3400 TGTACA T^GTACA
Tth111I 4270 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AseI 270 ATTAAT AT^TAAT
786 ATTAAT
AsnI 270 ATTAAT AT^TAAT
786 ATTAAT
Asp700I 714 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3991 GAATTGTTTC
BciVI 290 GGATAC GTATCC(6/5)
4872 GTATCC
BpmI 1231 CTCCAG CTGGAG(16/14)
460 CTGGAG
BsgI 1272 GTGCAG GTGCAG(16/14)
1359 GTGCAG
BspHI 3156 TCATGA T^CATGA
3873 TCATGA
BsrFI 2149 GCCGGT [GA]^CCGG[CT]
2664 GCCGGC
BstEII 2243 GGTTACC G^GT[ACGT]ACC
3136 GGTTACC
BstPI 2243 GGTTACC G^GT[ACGT]ACC
3136 GGTTACC
CfoI 2668 GCGC GCG^C
2683 GCGC
Cfr10I 2149 GCCGGT [GA]^CCGG[CT]
2664 GCCGGC
EcoO65I 2243 GGTTACC G^GT[ACGT]ACC
3136 GGTTACC
EcoT22I 899 ATGCAT ATGCA^T
3174 ATGCAT
HaeII 2667 GGCGCT [GA]GCGC^[CT]
2682 AGCGCC
HhaI 2668 GCGC GCG^C
2683 GCGC
HinP1I 2668 GCGC G^CGC
2683 GCGC
HindIII 3896 AAGCTT A^AGCTT
4017 AAGCTT
MfeI 334 CAATTG C^AATTG
3823 CAATTG
MunI 334 CAATTG C^AATTG
3823 CAATTG
NsiI 899 ATGCAT ATGCA^T
3174 ATGCAT
PshBI 270 ATTAAT AT^TAAT
786 ATTAAT
RcaI 3156 TCATGA T^CATGA
3873 TCATGA
SapI 2409 GAAGAGC GCTCTTC(1/4)
2618 GAAGAGC
ScaI 2864 AGTACT AGT^ACT
3065 AGTACT
SpeI 3661 ACTAGT A^CTAGT
4635 ACTAGT
XmnI 714 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3991 GAATTGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AvaII BamHI
BanI BanII BbsI BbvI BclI
BfaI BmyI BpuAI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspLU11I BspMI BsrDI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraI DraII
DsaI EarI EcoO109I EcoRII EcoT14I
FbaI Fnu4HI FokI HaeIII HapII
HgaI HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NcoI
NdeI NdeII NlaIII NlaIV NspI
PflMI PleI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII