##################################
# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII Acc65I AccI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI Asp718I AspEI AvaII AviII
AvrII BbeI BbrPI BbvCI BciVI
BclI BfrI BlnI BsaAI BsiEI
BsiWI BsmBI Bsp1407I BspDI BsrBI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HpaI KasI
KpnI MluI NarI NcoI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PpuMI
PshAI PvuI RsrII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccIII 2469 TCCGGA T^CCGGA
AclI 1194 AACGTT AA^CGTT
AcyI 17 GGCGTC G[GA]^CG[CT]C
AflIII 2335 ACATGT A^C[GA][CT]GT
AlwNI 2189 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 3407 GGGCCC GGGCC^C
AseI 3206 ATTAAT AT^TAAT
AsnI 3206 ATTAAT AT^TAAT
AspI 541 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1741 TGGCCA TGG^CCA
BamHI 3328 GGATCC G^GATCC
BanI 58 GGTGCC G^G[CT][GA]CC
BcgI 219 GCAAGAAAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 9 GCCTCCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 3218 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 3218 GCTAAGC GC^T[ACGT]AGC
BsaBI 1661 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 17 GGCGTC G[GA]^CG[CT]C
BsaWI 2469 TCCGGA [AT]^CCGG[AT]
BseAI 2469 TCCGGA T^CCGGA
BsgI 510 GTGCAG GTGCAG(16/14)
BspEI 2469 TCCGGA T^CCGGA
BspLU11I 2335 ACATGT A^CATGT
BspMI 1473 ACCTGC ACCTGC(4/8)
BsrFI 154 GCCGGC [GA]^CCGG[CT]
BstAPI 2230 GCAACAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2787 CCAAAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 3218 GCTAAGC GC^T[ACGT]AGC
Cfr10I 154 GCCGGC [GA]^CCGG[CT]
DraII 3406 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoNI 1651 CCTGGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 3406 AGGGCCC [GA]G^G[ACGT]CC[CT]
HaeII 3471 AGCGCC [GA]GCGC^[CT]
HgaI 18 GCGTC GACGC(5/10)
Hin1I 17 GGCGTC G[GA]^CG[CT]C
KspI 47 CCGCGG CCGC^GG
MamI 1661 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1741 TGGCCA TGG^CCA
MroI 2469 TCCGGA T^CCGGA
MscI 1741 TGGCCA TGG^CCA
NaeI 154 GCCGGC GCC^GGC
NgoMIV 154 GCCGGC G^CCGGC
NheI 1177 GCTAGC G^CTAGC
PflFI 541 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 3206 ATTAAT AT^TAAT
Psp1406I 1194 AACGTT AA^CGTT
SacI 2385 GAGCTC GAGCT^C
SacII 47 CCGCGG CCGC^GG
SapI 1382 GAAGAGC GCTCTTC(1/4)
ScaI 1702 AGTACT AGT^ACT
Tth111I 541 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 159 CCACCGCACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 48 CGCG CG^CG
145 CGCG
Asp700I 317 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1826 GAAAGGCTTC
AvaI 13 CCCGGG C^[CT]CG[GA]G
94 CCCGGG
BanII 2385 GAGCTC G[GA]GC[CT]^C
3407 GGGCCC
BsaI 1871 GAGACC GGTCTC(1/5)
3093 GAGACC
BsmI 1245 GAATGC GAATGC(1/-1)
2413 GCATTC
BsoBI 13 CCCGGG C^[CT]CG[GA]G
94 CCCGGG
BsrDI 2084 GCAATG GCAATG(2/0)
3306 GCAATG
BstUI 48 CGCG CG^CG
145 CGCG
DraI 256 TTTAAA TTT^AAA
2869 TTTAAA
DsaI 29 CCACGG C^C[GA][CT]GG
47 CCGCGG
MaeII 865 ACGT A^CGT
1195 ACGT
MvnI 48 CGCG CG^CG
145 CGCG
NdeI 1556 CATATG CA^TATG
2680 CATATG
PflMI 1369 CCAAAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1750 CCAAGATATGG
PstI 1788 CTGCAG CTGCA^G
2418 CTGCAG
SmaI 13 CCCGGG CCC^GGG
94 CCCGGG
SspI 355 AATATT AAT^ATT
1449 AATATT
Van91I 1369 CCAAAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1750 CCAAGATATGG
XbaI 548 TCTAGA T^CTAGA
1402 TCTAGA
XmaCI 13 CCCGGG C^CCGGG
94 CCCGGG
XmaI 13 CCCGGG C^CCGGG
94 CCCGGG
XmnI 317 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1826 GAAAGGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI BbsI BbvI BcnI
BfaI BglII BmyI BpmI BpuAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspHI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII EaeI
EarI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MfeI MflI MnlI MseI MslI
MspA1I MspI MunI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII