##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AclI AflII Aor51HI AscI AseI
AsnI BbrPI BciVI BfrI BlpI
Bpu1102I BsaBI Bsp1407I BsrGI BssHII
BssSI Bst1107I BstBI BstZ17I CelII
CpoI DraI EagI EclXI Eco47III
Eco52I EcoRI EcoRV FseI HindIII
HpaI MamI MfeI MluI MunI
NheI NotI NruI NspV PacI
PaeR7I PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SbfI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 1230 GACGTC GACGT^C
Acc65I 2392 GGTACC G^GTACC
AgeI 2260 ACCGGT A^CCGGT
AhdI 4186 GACTCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 2392 GGTACC G^GTACC
AspEI 4186 GACTCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4380 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2096 CCTAGG C^CTAGG
BcgI 1528 GCAAGAAGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2026 TGATCA T^GATCA
BglII 685 AGATCT A^GATCT
BlnI 2096 CCTAGG C^CTAGG
BsiWI 4319 CGTACG C^GTACG
BspDI 2662 ATCGAT AT^CGAT
BsrBI 2334 GAGCGG CCGCTC(-3/-3)
BsrDI 3129 CATTGC GCAATG(2/0)
BstAPI 986 GCACTCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI 2662 ATCGAT AT^CGAT
Eam1105I 4186 GACTCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 2412 ATGCAT ATGCA^T
FbaI 2026 TGATCA T^GATCA
HincII 4377 GTCGAC GT[CT]^[GA]AC
HindII 4377 GTCGAC GT[CT]^[GA]AC
KpnI 2392 GGTACC GGTAC^C
KspI 120 CCGCGG CCGC^GG
NsiI 2412 ATGCAT ATGCA^T
PflFI 4380 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2260 ACCGGT A^CCGGT
PshAI 652 GACCACGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 120 CCGCGG CCGC^GG
SalI 4377 GTCGAC G^TCGAC
SapI 95 GAAGAGC GCTCTTC(1/4)
ScaI 1222 AGTACT AGT^ACT
SmaI 2431 CCCGGG CCC^GGG
Tth111I 4380 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3865 TCTAGA T^CTAGA
XmaCI 2431 CCCGGG C^CCGGG
XmaI 2431 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccIII 449 TCCGGA T^CCGGA
2341 TCCGGA
AflIII 2956 ACATGT A^C[GA][CT]GT
3873 ACATGT
AviII 1090 TGCGCA TGC^GCA
1555 TGCGCA
BalI 1448 TGGCCA TGG^CCA
3888 TGGCCA
BamHI 1009 GGATCC G^GATCC
2338 GGATCC
BbvCI 4199 CCTCAGC CCTCAGC(-5/-2)
2172 GCTGAGG
BglI 1062 GCCATCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1587 GCCCAGGAGGC
BsaAI 2499 CACGTA [CT]AC^GT[GA]
3804 TACGTG
BseAI 449 TCCGGA T^CCGGA
2341 TCCGGA
BsiEI 72 CGGTCG CG[GA][CT]^CG
3500 CGACCG
BsmI 1721 GAATGC GAATGC(1/-1)
1898 GCATTC
BspEI 449 TCCGGA T^CCGGA
2341 TCCGGA
BspLU11I 2956 ACATGT A^CATGT
3873 ACATGT
BstEII 2133 GGTGACC G^GT[ACGT]ACC
3629 GGTCACC
BstPI 2133 GGTGACC G^GT[ACGT]ACC
3629 GGTCACC
BstXI 2051 CCAGAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3245 CCAGCAAGGTGG
DrdI 1278 GACAAGAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4120 GACTTCAGTGTC
EcoO65I 2133 GGTGACC G^GT[ACGT]ACC
3629 GGTCACC
FspI 1090 TGCGCA TGC^GCA
1555 TGCGCA
MluNI 1448 TGGCCA TGG^CCA
3888 TGGCCA
MroI 449 TCCGGA T^CCGGA
2341 TCCGGA
MscI 1448 TGGCCA TGG^CCA
3888 TGGCCA
NaeI 8 GCCGGC GCC^GGC
3851 GCCGGC
NdeI 1401 CATATG CA^TATG
2916 CATATG
NgoMIV 8 GCCGGC G^CCGGC
3851 GCCGGC
SacI 1305 GAGCTC GAGCT^C
1440 GAGCTC
XcmI 730 CCATCATAAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3817 CCATGCAGCCTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI Alw44I AlwI AlwNI
AocI ApaI ApaLI ApoI Asp700I
AspHI AvaI AvaII BanI BanII
BbeI BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspHI BspMI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RcaI RsaI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SfoI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII XmnI