##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
Aor51HI ApaI AscI Asp718I AviII
AvrII BanI BbeI BbrPI BbvCI
BciVI BlnI BlpI Bpu1102I BsaAI
BseAI BsiEI BsiWI BspDI BspEI
BspLU11I BspMI BssHII BssSI BstEII
BstPI BstXI CelII ClaI CpoI
DraIII DrdI EagI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
FseI FspI HincII HindII HindIII
HpaI KasI KpnI MluI MroI
NarI NcoI NotI NruI PacI
PaeR7I PinAI PmaCI PmlI PshAI
Psp1406I PvuI RsrII SalI SapI
SbfI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
StuI SwaI XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AflII 3133 CTTAAG C^TTAAG
AflIII 2185 ACACGT A^C[GA][CT]GT
Alw44I 2477 GTGCAC G^TGCAC
AocI 816 CCTGAGG CC^T[ACGT]AGG
ApaLI 2477 GTGCAC G^TGCAC
AseI 3774 ATTAAT AT^TAAT
AsnI 3774 ATTAAT AT^TAAT
Asp700I 2340 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 382 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 184 CCCGAG C^[CT]CG[GA]G
BcgI 1686 CGATGGCTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 464 TGATCA T^GATCA
BfrI 3133 CTTAAG C^TTAAG
BglI 182 GCCCCGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 2256 TCCGGT [AT]^CCGG[AT]
BseRI 2099 CTCCTC GAGGAG(10/8)
BsmBI 162 CGTCTC CGTCTC(1/5)
BsoBI 184 CCCGAG C^[CT]CG[GA]G
Bsp1407I 2556 TGTACA T^GTACA
BspHI 978 TCATGA T^CATGA
BsrBI 2333 CCGCTC CCGCTC(-3/-3)
BsrFI 20 GCCGGC [GA]^CCGG[CT]
BsrGI 2556 TGTACA T^GTACA
BstAPI 2361 GCAGAACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1931 TTCGAA TT^CGAA
Bsu36I 816 CCTGAGG CC^T[ACGT]AGG
Cfr10I 20 GCCGGC [GA]^CCGG[CT]
Eco81I 816 CCTGAGG CC^T[ACGT]AGG
EcoT22I 640 ATGCAT ATGCA^T
FbaI 464 TGATCA T^GATCA
HaeII 23 GGCGCT [GA]GCGC^[CT]
KspI 78 CCGCGG CCGC^GG
MfeI 1391 CAATTG C^AATTG
MunI 1391 CAATTG C^AATTG
NaeI 20 GCCGGC GCC^GGC
NdeI 1505 CATATG CA^TATG
NgoMIV 20 GCCGGC G^CCGGC
NheI 3118 GCTAGC G^CTAGC
NsiI 640 ATGCAT ATGCA^T
NspV 1931 TTCGAA TT^CGAA
PflFI 382 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmeI 1224 GTTTAAAC GTTT^AAAC
PshBI 3774 ATTAAT AT^TAAT
PstI 1283 CTGCAG CTGCA^G
RcaI 978 TCATGA T^CATGA
SacII 78 CCGCGG CCGC^GG
ScaI 1362 AGTACT AGT^ACT
SfuI 1931 TTCGAA TT^CGAA
SspBI 2556 TGTACA T^GTACA
Tth111I 382 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3765 TCTAGA T^CTAGA
XmnI 2340 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 845 GTATAC GT^[AC][GT]AC
3368 GTATAC
AcyI 160 GGCGTC G[GA]^CG[CT]C
811 GACGCC
AhdI 2316 GACCATTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3290 GACAGTTTGTC
AspEI 2316 GACCATTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3290 GACAGTTTGTC
BalI 1247 TGGCCA TGG^CCA
1480 TGGCCA
BamHI 193 GGATCC G^GATCC
1723 GGATCC
BglII 705 AGATCT A^GATCT
1575 AGATCT
BsaBI 706 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1576 GATCTTTATC
BsaHI 160 GGCGTC G[GA]^CG[CT]C
811 GACGCC
BsaI 1986 GAGACC GGTCTC(1/5)
2 GGTCTC
BsgI 63 GTGCAG GTGCAG(16/14)
1327 GTGCAG
BsrDI 1523 GCAATG GCAATG(2/0)
1917 GCAATG
Bst1107I 845 GTATAC GTA^TAC
3368 GTATAC
BstZ17I 845 GTATAC GTA^TAC
3368 GTATAC
EaeI 1247 TGGCCA [CT]^GGCC[GA]
1480 TGGCCA
Eam1105I 2316 GACCATTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3290 GACAGTTTGTC
FauI 1013 CCCGC CCCGC(4/6)
1176 GCGGG
HgaI 811 GACGC GACGC(5/10)
161 GCGTC
Hin1I 160 GGCGTC G[GA]^CG[CT]C
811 GACGCC
MamI 706 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1576 GATCTTTATC
MluNI 1247 TGGCCA TGG^CCA
1480 TGGCCA
MscI 1247 TGGCCA TGG^CCA
1480 TGGCCA
NspI 2737 ACATGC [GA]CATG^[CT]
3257 ACATGC
PpuMI 1341 AGGTCCT [GA]G^G[AT]CC[CT]
2168 AGGTCCC
SacI 863 GAGCTC GAGCT^C
1867 GAGCTC
SspI 2910 AATATT AAT^ATT
3166 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaII
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII