##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
Acc65I AclI AflII AgeI Aor51HI
AscI Asp718I AspI AviII AvrII
BanI BbeI BbrPI BbvCI BfrI
BlnI BlpI Bpu1102I BsaAI BsiWI
BspHI BsrBI BsrFI BssHII BssSI
BstBI CelII Cfr10I CpoI Eco47III
EcoRV EcoT22I FseI FspI KasI
KpnI KspI MluI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmaCI PmlI PshAI Psp1406I PvuI
RcaI RsrII SacI SacII SapI
ScaI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI SwaI Tth111I
XbaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AatII 3351 GACGTC GACGT^C
AccIII 48 TCCGGA T^CCGGA
AflIII 4402 ACATGT A^C[GA][CT]GT
AhdI 3351 GACGTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 56 GTGCAC G^TGCAC
AocI 3230 CCTCAGG CC^T[ACGT]AGG
ApaI 3489 GGGCCC GGGCC^C
ApaLI 56 GTGCAC G^TGCAC
AseI 4488 ATTAAT AT^TAAT
AsnI 4488 ATTAAT AT^TAAT
AspEI 3351 GACGTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BcgI 1229 GCAGATAACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1077 TGATCA T^GATCA
BfaI 2957 CTAG C^TAG
BsaI 2259 GAGACC GGTCTC(1/5)
BseAI 48 TCCGGA T^CCGGA
BsgI 3983 CTGCAC GTGCAG(16/14)
BsiEI 789 CGGCCG CG[GA][CT]^CG
BsmBI 2238 GAGACG CGTCTC(1/5)
BsmI 4390 GCATTC GAATGC(1/-1)
Bsp1407I 1947 TGTACA T^GTACA
BspDI 2816 ATCGAT AT^CGAT
BspEI 48 TCCGGA T^CCGGA
BspLU11I 4402 ACATGT A^CATGT
BsrGI 1947 TGTACA T^GTACA
Bst1107I 3640 GTATAC GTA^TAC
BstEII 1850 GGTTACC G^GT[ACGT]ACC
BstPI 1850 GGTTACC G^GT[ACGT]ACC
BstZ17I 3640 GTATAC GTA^TAC
Bsu36I 3230 CCTCAGG CC^T[ACGT]AGG
ClaI 2816 ATCGAT AT^CGAT
DrdI 3901 GACCTCAACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 789 CGGCCG C^GGCCG
Eam1105I 3351 GACGTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 789 CGGCCG C^GGCCG
Eco52I 789 CGGCCG C^GGCCG
Eco81I 3230 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1850 GGTTACC G^GT[ACGT]ACC
FbaI 1077 TGATCA T^GATCA
HpaI 1249 GTTAAC GTT^AAC
MaeI 2957 CTAG C^TAG
MroI 48 TCCGGA T^CCGGA
NcoI 4369 CCATGG C^CATGG
PmeI 3009 GTTTAAAC GTTT^AAAC
PshBI 4488 ATTAAT AT^TAAT
SalI 1878 GTCGAC G^TCGAC
SbfI 2671 CCTGCAGG CCTGCA^GG
SexAI 3050 ACCAGGT A^CC[AT]GGT
Sse8387I 2671 CCTGCAGG CCTGCA^GG
SspBI 1947 TGTACA T^GTACA
StuI 2601 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 661 CGCG CG^CG
663 CGCG
AcyI 2240 GACGCC G[GA]^CG[CT]C
3351 GACGTC
BalI 1591 TGGCCA TGG^CCA
3445 TGGCCA
BglI 371 GCCAAGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
803 GCCAACAGGGC
BsaHI 2240 GACGCC G[GA]^CG[CT]C
3351 GACGTC
BsaWI 48 TCCGGA [AT]^CCGG[AT]
237 ACCGGA
BstUI 661 CGCG CG^CG
663 CGCG
BstXI 3288 CCAGAGGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4379 CCATCACTTTGG
DraIII 3431 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
3950 CACTCGGTG
DsaI 2112 CCGTGG C^C[GA][CT]GG
4369 CCATGG
EarI 695 GAAGAG CTCTTC(1/4)
2012 GAAGAG
FauI 717 GCGGG CCCGC(4/6)
2889 GCGGG
HaeII 3465 GGCGCT [GA]GCGC^[CT]
3535 AGCGCC
Hin1I 2240 GACGCC G[GA]^CG[CT]C
3351 GACGTC
HincII 1249 GTTAAC GT[CT]^[GA]AC
1878 GTCGAC
HindII 1249 GTTAAC GT[CT]^[GA]AC
1878 GTCGAC
Ksp632I 695 GAAGAG CTCTTC(1/4)
2012 GAAGAG
MfeI 2385 CAATTG C^AATTG
2555 CAATTG
MluNI 1591 TGGCCA TGG^CCA
3445 TGGCCA
MscI 1591 TGGCCA TGG^CCA
3445 TGGCCA
MunI 2385 CAATTG C^AATTG
2555 CAATTG
MvnI 661 CGCG CG^CG
663 CGCG
PaeR7I 2052 CTCGAG C^TCGAG
4300 CTCGAG
PflMI 126 CCAGAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2084 CCAACCTTTGG
PpuMI 245 AGGACCT [GA]G^G[AT]CC[CT]
3899 GGGACCT
Van91I 126 CCAGAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2084 CCAACCTTTGG
XcmI 2599 CCAGGCCTCATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3166 CCAGGCCAATCATGG
XhoI 2052 CTCGAG C^TCGAG
4300 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaI AvaII BamHI BanII BbsI
BbvI BciVI BcnI BglII BmyI
BpmI BpuAI BsaBI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrDI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII EaeI EcoNI EcoO109I
EcoRI EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HindIII HinfI HpaII HphI ItaI
MaeII MaeIII MamI MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmaCI XmaI XmnI