##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
Acc65I AccII AgeI AhdI Aor51HI
ApaI AscI Asp718I AspEI AviII
AvrII BbeI BbrPI BcgI BciVI
BclI BlnI BsaBI BsaI BsiEI
BsiWI BsmBI BspDI BspLU11I BsrBI
BssHII BstAPI BstBI BstUI ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco47III Eco52I FauI FbaI FseI
FspI KasI KpnI KspI MamI
MfeI MluI MunI MvnI NaeI
NarI NgoMIV NotI NruI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PvuI PvuII RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SwaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 3205 GACGTC GACGT^C
AccIII 1573 TCCGGA T^CCGGA
AflII 1168 CTTAAG C^TTAAG
AflIII 2698 ACGTGT A^C[GA][CT]GT
Asp700I 3427 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 3515 CCCGAG C^[CT]CG[GA]G
BamHI 1593 GGATCC G^GATCC
BanII 2336 GAGCCC G[GA]GC[CT]^C
BbvCI 64 CCTCAGC CCTCAGC(-5/-2)
BcnI 2872 CCCGG CC^[GC]GG
BfrI 1168 CTTAAG C^TTAAG
BglII 1055 AGATCT A^GATCT
BlpI 1046 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1046 GCTCAGC GC^T[ACGT]AGC
BsaAI 3452 TACGTG [CT]AC^GT[GA]
BsaWI 1573 TCCGGA [AT]^CCGG[AT]
BseAI 1573 TCCGGA T^CCGGA
BsmI 2879 GAATGC GAATGC(1/-1)
BsoBI 3515 CCCGAG C^[CT]CG[GA]G
BspEI 1573 TCCGGA T^CCGGA
BspMI 154 GCAGGT ACCTGC(4/8)
BsrFI 24 GCCGGT [GA]^CCGG[CT]
Bst1107I 504 GTATAC GTA^TAC
BstEII 632 GGTCACC G^GT[ACGT]ACC
BstPI 632 GGTCACC G^GT[ACGT]ACC
BstZ17I 504 GTATAC GTA^TAC
CelII 1046 GCTCAGC GC^T[ACGT]AGC
Cfr10I 24 GCCGGT [GA]^CCGG[CT]
DraI 4160 TTTAAA TTT^AAA
DraIII 935 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 3393 CCATGG C^C[GA][CT]GG
EcoO65I 632 GGTCACC G^GT[ACGT]ACC
HaeII 3582 GGCGCT [GA]GCGC^[CT]
MroI 1573 TCCGGA T^CCGGA
NciI 2872 CCCGG CC^[GC]GG
NcoI 3393 CCATGG C^CATGG
NdeI 3728 CATATG CA^TATG
NheI 3931 GCTAGC G^CTAGC
PstI 2647 CTGCAG CTGCA^G
SapI 2333 GAAGAGC GCTCTTC(1/4)
SphI 1003 GCATGC GCATG^C
TaqI 3273 TCGA T^CGA
TthHB8I 3273 TCGA T^CGA
XcmI 3860 CCAAGACATTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 3427 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 504 GTATAC GT^[AC][GT]AC
3449 GTCTAC
AclI 806 AACGTT AA^CGTT
4046 AACGTT
Alw44I 2547 GTGCAC G^TGCAC
3595 GTGCAC
AocI 357 CCTTAGG CC^T[ACGT]AGG
4482 CCTCAGG
ApaLI 2547 GTGCAC G^TGCAC
3595 GTGCAC
BglI 3389 GCCACCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3904 GCCTTTAGGGC
Bsu36I 357 CCTTAGG CC^T[ACGT]AGG
4482 CCTCAGG
Eco81I 357 CCTTAGG CC^T[ACGT]AGG
4482 CCTCAGG
EcoNI 2528 CCTTTGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3280 CCTCCGCCAGG
EcoRI 2383 GAATTC G^AATTC
2461 GAATTC
EcoRV 1301 GATATC GAT^ATC
1397 GATATC
EcoT22I 1467 ATGCAT ATGCA^T
3038 ATGCAT
HindIII 2037 AAGCTT A^AGCTT
3506 AAGCTT
HpaI 224 GTTAAC GTT^AAC
3106 GTTAAC
MspA1I 2194 CCGCTG C[AC]G^C[GT]G
2893 CCGCTG
NsiI 1467 ATGCAT ATGCA^T
3038 ATGCAT
NspI 508 ACATGC [GA]CATG^[CT]
1003 GCATGC
PshAI 410 GACATCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
626 GACATAGGTC
Psp1406I 806 AACGTT AA^CGTT
4046 AACGTT
SspI 2295 AATATT AAT^ATT
4171 AATATT
XbaI 487 TCTAGA T^CTAGA
2572 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApoI AseI AsnI
AspHI AspI AvaII BalI BanI
BbsI BbvI BfaI BmyI BpmI
BpuAI BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I Bsp1407I BspHI BsrDI BsrGI
BsrI BssKI BssSI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspI
MvaI MwoI NdeII NlaIII NlaIV
PflFI PflMI PleI PpuMI PshBI
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI StuI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I Van91I XhoII