##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
Acc65I AccIII AclI AgeI AhdI
Alw44I Aor51HI ApaLI AscI Asp718I
AspEI AviII BlpI Bpu1102I BsaWI
BseAI BsiEI BsiWI BsmBI BspDI
BspEI BsrBI BssHII Bst1107I BstEII
BstPI BstZ17I CelII ClaI CpoI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I FseI FspI KpnI KspI
MroI NdeI NheI NotI NruI
PacI PinAI PmeI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
SfiI SgrAI SnaBI SpeI Sse8387I
SwaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 4029 GACGTC GACGT^C
AflII 6494 CTTAAG C^TTAAG
ApaI 1839 GGGCCC GGGCC^C
AvrII 5053 CCTAGG C^CTAGG
BamHI 3855 GGATCC G^GATCC
BbeI 812 GGCGCC GGCGC^C
BbrPI 1559 CACGTG CAC^GTG
BbvCI 1814 GCTGAGG CCTCAGC(-5/-2)
BciVI 4901 GGATAC GTATCC(6/5)
BclI 7036 TGATCA T^GATCA
BfrI 6494 CTTAAG C^TTAAG
BlnI 5053 CCTAGG C^CTAGG
BsaAI 1559 CACGTG [CT]AC^GT[GA]
Bsp1407I 4408 TGTACA T^GTACA
BspLU11I 5920 ACATGT A^CATGT
BsrFI 815 GCCGGC [GA]^CCGG[CT]
BsrGI 4408 TGTACA T^GTACA
Cfr10I 815 GCCGGC [GA]^CCGG[CT]
EcoRI 1220 GAATTC G^AATTC
EcoRV 3928 GATATC GAT^ATC
FbaI 7036 TGATCA T^GATCA
HaeII 812 GGCGCC [GA]GCGC^[CT]
HincII 6269 GTTAAC GT[CT]^[GA]AC
HindII 6269 GTTAAC GT[CT]^[GA]AC
HpaI 6269 GTTAAC GTT^AAC
KasI 812 GGCGCC G^GCGCC
MluI 2236 ACGCGT A^CGCGT
NaeI 815 GCCGGC GCC^GGC
NarI 812 GGCGCC GG^CGCC
NgoMIV 815 GCCGGC G^CCGGC
PaeR7I 1167 CTCGAG C^TCGAG
PmaCI 1559 CACGTG CAC^GTG
PmlI 1559 CACGTG CAC^GTG
PshAI 6099 GACCAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 4570 GAGCTC GAGCT^C
SexAI 566 ACCTGGT A^CC[AT]GGT
SfoI 812 GGCGCC GGC^GCC
SphI 6573 GCATGC GCATG^C
SspBI 4408 TGTACA T^GTACA
XhoI 1167 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 3061 GTCTAC GT^[AC][GT]AC
6874 GTAGAC
AccII 2237 CGCG CG^CG
3507 CGCG
AspI 1760 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5153 GACTATGTC
BglI 2086 GCCCTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
5114 GCCCGGCTGGC
BsaBI 3751 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4931 GATAACTATC
BsaI 5093 GAGACC GGTCTC(1/5)
1150 GGTCTC
BsmI 453 GAATGC GAATGC(1/-1)
5241 GAATGC
BssSI 870 CTCGTG CACGAG(-5/-1)
5452 CTCGTG
BstBI 1329 TTCGAA TT^CGAA
5817 TTCGAA
BstUI 2237 CGCG CG^CG
3507 CGCG
DraIII 5876 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
6921 CACACTGTG
DrdI 3188 GACTTCACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4733 GACATGGATGTC
EcoT22I 1486 ATGCAT ATGCA^T
5334 ATGCAT
FauI 4080 CCCGC CCCGC(4/6)
3508 GCGGG
MamI 3751 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4931 GATAACTATC
MfeI 741 CAATTG C^AATTG
1374 CAATTG
MunI 741 CAATTG C^AATTG
1374 CAATTG
MvnI 2237 CGCG CG^CG
3507 CGCG
NsiI 1486 ATGCAT ATGCA^T
5334 ATGCAT
NspV 1329 TTCGAA TT^CGAA
5817 TTCGAA
PflFI 1760 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5153 GACTATGTC
PpuMI 3362 GGGTCCC [GA]G^G[AT]CC[CT]
4577 GGGTCCC
PvuII 77 CAGCTG CAG^CTG
1047 CAGCTG
ScaI 2311 AGTACT AGT^ACT
2937 AGTACT
SfuI 1329 TTCGAA TT^CGAA
5817 TTCGAA
SmaI 3480 CCCGGG CCC^GGG
5097 CCCGGG
Tth111I 1760 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5153 GACTATGTC
XbaI 106 TCTAGA T^CTAGA
3211 TCTAGA
XmaCI 3480 CCCGGG C^CCGGG
5097 CCCGGG
XmaI 3480 CCCGGG C^CCGGG
5097 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
AseI AsnI Asp700I AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BcgI BcnI BfaI BglII
BmyI BpmI BpuAI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspHI
BspMI BsrDI BsrI BssKI BstAPI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PshBI PstI RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmnI