##################################
# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Aor51HI ApaI
AscI Asp718I AspEI AviII BbeI
BbrPI BciVI BclI BfrI BglI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BseAI BsiEI BsiWI BspDI
BspEI BspHI BspMI BsrFI BssHII
BssSI Bst1107I BstBI BstZ17I CelII
Cfr10I ClaI CpoI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO109I FbaI FseI FspI
Hin1I HpaI KasI KpnI KspI
MamI MluI MroI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PaeR7I PflMI PinAI
PmaCI PmeI PmlI PpuMI Psp1406I
PstI PvuI RcaI RsrII SacII
SalI SapI SbfI SexAI SfcI
SfiI SfoI SfuI SgrAI SnaBI
Sse8387I StuI SwaI Van91I XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccII 3871 CGCG CG^CG
Alw44I 831 GTGCAC G^TGCAC
ApaLI 831 GTGCAC G^TGCAC
AseI 2348 ATTAAT AT^TAAT
AsnI 2348 ATTAAT AT^TAAT
Asp700I 455 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 502 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3244 CCTAGG C^CTAGG
BamHI 214 GGATCC G^GATCC
BanI 1693 GGCACC G^G[CT][GA]CC
BbvCI 3901 GCTGAGG CCTCAGC(-5/-2)
BglII 1521 AGATCT A^GATCT
BlnI 3244 CCTAGG C^CTAGG
BsaWI 985 ACCGGA [AT]^CCGG[AT]
BseRI 3336 GAGGAG GAGGAG(10/8)
BsgI 232 CTGCAC GTGCAG(16/14)
BsmBI 3832 CGTCTC CGTCTC(1/5)
BsrBI 1697 CCGCTC CCGCTC(-3/-3)
BstUI 3871 CGCG CG^CG
DraI 2795 TTTAAA TTT^AAA
DsaI 287 CCACGG C^C[GA][CT]GG
EcoNI 1997 CCTGCATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2741 GAATTC G^AATTC
EcoT22I 2656 ATGCAT ATGCA^T
HaeII 1770 GGCGCT [GA]GCGC^[CT]
HgaI 502 GACGC GACGC(5/10)
MvnI 3871 CGCG CG^CG
NsiI 2656 ATGCAT ATGCA^T
PacI 3198 TTAATTAA TTAAT^TAA
PflFI 502 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 3235 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2348 ATTAAT AT^TAAT
PvuII 207 CAGCTG CAG^CTG
SacI 1936 GAGCTC GAGCT^C
ScaI 3010 AGTACT AGT^ACT
SmaI 732 CCCGGG CCC^GGG
SmlI 1504 CTCAAG C^T[CT][GA]AG
SpeI 2803 ACTAGT A^CTAGT
SphI 2658 GCATGC GCATG^C
Tth111I 502 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 732 CCCGGG C^CCGGG
XmaI 732 CCCGGG C^CCGGG
XmnI 455 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 1951 GTAGAC GT^[AC][GT]AC
3180 GTAGAC
AflIII 1570 ACATGT A^C[GA][CT]GT
1654 ACATGT
AlwNI 82 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
2097 CAGCTTCTG
AocI 2570 CCTTAGG CC^T[ACGT]AGG
3785 CCTGAGG
AspHI 831 GTGCAC G[AT]GC[AT]^C
1936 GAGCTC
AvaII 53 GGACC G^G[AT]CC
893 GGACC
BanII 1936 GAGCTC G[GA]GC[CT]^C
3215 GGGCTC
BcgI 982 CGAACCGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3907 GCAGGAGAATCG
BsiHKAI 831 GTGCAC G[AT]GC[AT]^C
1936 GAGCTC
BsmFI 318 GTCCC GGGAC(10/14)
2668 GTCCC
Bsp1407I 1646 TGTACA T^GTACA
1799 TGTACA
BspLU11I 1570 ACATGT A^CATGT
1654 ACATGT
BsrGI 1646 TGTACA T^GTACA
1799 TGTACA
BstAPI 505 GCAGTCTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3934 GCAGAGGTTGC
Bsu36I 2570 CCTTAGG CC^T[ACGT]AGG
3785 CCTGAGG
CfoI 1771 GCGC GCG^C
3870 GCGC
Eco81I 2570 CCTTAGG CC^T[ACGT]AGG
3785 CCTGAGG
EcoRV 247 GATATC GAT^ATC
3051 GATATC
FauI 820 CCCGC CCCGC(4/6)
1140 GCGGG
HhaI 1771 GCGC GCG^C
3870 GCGC
HinP1I 1771 GCGC G^CGC
3870 GCGC
HincII 390 GTTGAC GT[CT]^[GA]AC
3568 GTCAAC
HindII 390 GTTGAC GT[CT]^[GA]AC
3568 GTCAAC
MfeI 2748 CAATTG C^AATTG
3329 CAATTG
MspA1I 207 CAGCTG C[AC]G^C[GT]G
1706 CAGCGG
MunI 2748 CAATTG C^AATTG
3329 CAATTG
SspBI 1646 TGTACA T^GTACA
1799 TGTACA
TaqI 981 TCGA T^CGA
1885 TCGA
TthHB8I 981 TCGA T^CGA
1885 TCGA
XbaI 2012 TCTAGA T^CTAGA
2428 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaI BalI BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BsiYI BslI BsmAI BsmI
BsoBI Bsp1286I BsrDI BsrI BssKI
BstEII BstF5I BstNI BstPI BstXI
BstYI Cac8I Cfr13I DdeI DpnII
EaeI EarI EcoO65I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SspI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XcmI XhoII