################################## # NO CUT ENZYMES ( 100 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AgeI AhdI Aor51HI ApaI AscI Asp718I AspEI AviII BbeI BbrPI BciVI BclI BfrI BglI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BseAI BsiEI BsiWI BspDI BspEI BspHI BspMI BsrFI BssHII BssSI Bst1107I BstBI BstZ17I CelII Cfr10I ClaI CpoI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO109I FbaI FseI FspI Hin1I HpaI KasI KpnI KspI MamI MluI MroI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspV PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI Psp1406I PstI PvuI RcaI RsrII SacII SalI SapI SbfI SexAI SfcI SfiI SfoI SfuI SgrAI SnaBI Sse8387I StuI SwaI Van91I XhoI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccII 3871 CGCG CG^CG Alw44I 831 GTGCAC G^TGCAC ApaLI 831 GTGCAC G^TGCAC AseI 2348 ATTAAT AT^TAAT AsnI 2348 ATTAAT AT^TAAT Asp700I 455 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 502 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 3244 CCTAGG C^CTAGG BamHI 214 GGATCC G^GATCC BanI 1693 GGCACC G^G[CT][GA]CC BbvCI 3901 GCTGAGG CCTCAGC(-5/-2) BglII 1521 AGATCT A^GATCT BlnI 3244 CCTAGG C^CTAGG BsaWI 985 ACCGGA [AT]^CCGG[AT] BseRI 3336 GAGGAG GAGGAG(10/8) BsgI 232 CTGCAC GTGCAG(16/14) BsmBI 3832 CGTCTC CGTCTC(1/5) BsrBI 1697 CCGCTC CCGCTC(-3/-3) BstUI 3871 CGCG CG^CG DraI 2795 TTTAAA TTT^AAA DsaI 287 CCACGG C^C[GA][CT]GG EcoNI 1997 CCTGCATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 2741 GAATTC G^AATTC EcoT22I 2656 ATGCAT ATGCA^T HaeII 1770 GGCGCT [GA]GCGC^[CT] HgaI 502 GACGC GACGC(5/10) MvnI 3871 CGCG CG^CG NsiI 2656 ATGCAT ATGCA^T PacI 3198 TTAATTAA TTAAT^TAA PflFI 502 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 3235 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 2348 ATTAAT AT^TAAT PvuII 207 CAGCTG CAG^CTG SacI 1936 GAGCTC GAGCT^C ScaI 3010 AGTACT AGT^ACT SmaI 732 CCCGGG CCC^GGG SmlI 1504 CTCAAG C^T[CT][GA]AG SpeI 2803 ACTAGT A^CTAGT SphI 2658 GCATGC GCATG^C Tth111I 502 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 732 CCCGGG C^CCGGG XmaI 732 CCCGGG C^CCGGG XmnI 455 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccI 1951 GTAGAC GT^[AC][GT]AC 3180 GTAGAC AflIII 1570 ACATGT A^C[GA][CT]GT 1654 ACATGT AlwNI 82 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG 2097 CAGCTTCTG AocI 2570 CCTTAGG CC^T[ACGT]AGG 3785 CCTGAGG AspHI 831 GTGCAC G[AT]GC[AT]^C 1936 GAGCTC AvaII 53 GGACC G^G[AT]CC 893 GGACC BanII 1936 GAGCTC G[GA]GC[CT]^C 3215 GGGCTC BcgI 982 CGAACCGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 3907 GCAGGAGAATCG BsiHKAI 831 GTGCAC G[AT]GC[AT]^C 1936 GAGCTC BsmFI 318 GTCCC GGGAC(10/14) 2668 GTCCC Bsp1407I 1646 TGTACA T^GTACA 1799 TGTACA BspLU11I 1570 ACATGT A^CATGT 1654 ACATGT BsrGI 1646 TGTACA T^GTACA 1799 TGTACA BstAPI 505 GCAGTCTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3934 GCAGAGGTTGC Bsu36I 2570 CCTTAGG CC^T[ACGT]AGG 3785 CCTGAGG CfoI 1771 GCGC GCG^C 3870 GCGC Eco81I 2570 CCTTAGG CC^T[ACGT]AGG 3785 CCTGAGG EcoRV 247 GATATC GAT^ATC 3051 GATATC FauI 820 CCCGC CCCGC(4/6) 1140 GCGGG HhaI 1771 GCGC GCG^C 3870 GCGC HinP1I 1771 GCGC G^CGC 3870 GCGC HincII 390 GTTGAC GT[CT]^[GA]AC 3568 GTCAAC HindII 390 GTTGAC GT[CT]^[GA]AC 3568 GTCAAC MfeI 2748 CAATTG C^AATTG 3329 CAATTG MspA1I 207 CAGCTG C[AC]G^C[GT]G 1706 CAGCGG MunI 2748 CAATTG C^AATTG 3329 CAATTG SspBI 1646 TGTACA T^GTACA 1799 TGTACA TaqI 981 TCGA T^CGA 1885 TCGA TthHB8I 981 TCGA T^CGA 1885 TCGA XbaI 2012 TCTAGA T^CTAGA 2428 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 85 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AvaI BalI BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BsiYI BslI BsmAI BsmI BsoBI Bsp1286I BsrDI BsrI BssKI BstEII BstF5I BstNI BstPI BstXI BstYI Cac8I Cfr13I DdeI DpnII EaeI EarI EcoO65I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI RsaI Sau3AI Sau96I ScrFI SfaNI SspI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XcmI XhoII