##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflIII AgeI AhdI Alw44I Aor51HI
ApaLI AscI Asp718I AspEI AviII
AvrII BamHI BbrPI BclI BlnI
BsaBI BsaWI BseAI BsiEI BsiWI
BspDI BspEI BspLU11I BsrBI BssHII
BssSI BstBI BstEII BstPI BstUI
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRV
FbaI FseI FspI HgaI HpaI
KpnI KspI MamI MluI MroI
MvnI NaeI NcoI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI SfiI SfuI SgrAI
SmaI SnaBI Sse8387I StuI SwaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AcyI 4627 GGCGCC G[GA]^CG[CT]C
AocI 4454 CCTGAGG CC^T[ACGT]AGG
ApaI 4429 GGGCCC GGGCC^C
AseI 592 ATTAAT AT^TAAT
AsnI 592 ATTAAT AT^TAAT
Asp700I 353 GAAGGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 4662 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 2429 CCCGAG C^[CT]CG[GA]G
BalI 4481 TGGCCA TGG^CCA
BanII 4429 GGGCCC G[GA]GC[CT]^C
BbeI 4627 GGCGCC GGCGC^C
BbsI 4254 GTCTTC GAAGAC(2/6)
BcgI 4577 GCAGGAAAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 4528 CCGGG CC^[GC]GG
BglI 4619 GCCGAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1850 AGATCT A^GATCT
BlpI 252 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 252 GCTTAGC GC^T[ACGT]AGC
BpuAI 4254 GTCTTC GAAGAC(2/6)
BsaAI 3510 CACGTA [CT]AC^GT[GA]
BsaHI 4627 GGCGCC G[GA]^CG[CT]C
BsgI 864 CTGCAC GTGCAG(16/14)
BsoBI 2429 CCCGAG C^[CT]CG[GA]G
BspHI 3341 TCATGA T^CATGA
BsrFI 90 GCCGGT [GA]^CCGG[CT]
BstAPI 1573 GCAAGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1999 CCAGGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 4454 CCTGAGG CC^T[ACGT]AGG
CelII 252 GCTTAGC GC^T[ACGT]AGC
Cfr10I 90 GCCGGT [GA]^CCGG[CT]
DraIII 2614 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 137 CCGTGG C^C[GA][CT]GG
EarI 1271 GAAGAG CTCTTC(1/4)
Eco81I 4454 CCTGAGG CC^T[ACGT]AGG
EcoT14I 1042 CCAAGG C^C[AT][AT]GG
EcoT22I 3785 ATGCAT ATGCA^T
FauI 4442 GCGGG CCCGC(4/6)
Hin1I 4627 GGCGCC G[GA]^CG[CT]C
HindIII 4147 AAGCTT A^AGCTT
KasI 4627 GGCGCC G^GCGCC
Ksp632I 1271 GAAGAG CTCTTC(1/4)
MfeI 1356 CAATTG C^AATTG
MluNI 4481 TGGCCA TGG^CCA
MscI 4481 TGGCCA TGG^CCA
MunI 1356 CAATTG C^AATTG
NarI 4627 GGCGCC GG^CGCC
NciI 4528 CCGGG CC^[GC]GG
NdeI 3641 CATATG CA^TATG
NsiI 3785 ATGCAT ATGCA^T
PflFI 4662 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 940 GACACCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 592 ATTAAT AT^TAAT
RcaI 3341 TCATGA T^CATGA
SexAI 772 ACCAGGT A^CC[AT]GGT
SfoI 4627 GGCGCC GGC^GCC
StyI 1042 CCAAGG C^C[AT][AT]GG
Tth111I 4662 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1042 CCAAGGACCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 353 GAAGGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AflII 2026 CTTAAG C^TTAAG
2824 CTTAAG
AlwNI 243 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
873 CAGCAACTG
BanI 1510 GGTGCC G^G[CT][GA]CC
4627 GGCGCC
BciVI 1463 GGATAC GTATCC(6/5)
2241 GTATCC
BfrI 2026 CTTAAG C^TTAAG
2824 CTTAAG
Bsp1407I 1937 TGTACA T^GTACA
2132 TGTACA
BsrGI 1937 TGTACA T^GTACA
2132 TGTACA
Bst1107I 238 GTATAC GTA^TAC
3513 GTATAC
BstZ17I 238 GTATAC GTA^TAC
3513 GTATAC
CfoI 839 GCGC GCG^C
4628 GCGC
EaeI 1316 CGGCCA [CT]^GGCC[GA]
4481 TGGCCA
EcoRI 804 GAATTC G^AATTC
2667 GAATTC
HaeII 838 AGCGCC [GA]GCGC^[CT]
4627 GGCGCC
HapII 91 CCGG C^CGG
4528 CCGG
HhaI 839 GCGC GCG^C
4628 GCGC
HinP1I 839 GCGC G^CGC
4628 GCGC
HincII 2312 GTTGAC GT[CT]^[GA]AC
3705 GTCAAC
HindII 2312 GTTGAC GT[CT]^[GA]AC
3705 GTCAAC
HpaII 91 CCGG C^CGG
4528 CCGG
MspI 91 CCGG C^CGG
4528 CCGG
PpuMI 244 AGGACCT [GA]G^G[AT]CC[CT]
1045 AGGACCT
SspBI 1937 TGTACA T^GTACA
2132 TGTACA
SspI 3051 AATATT AAT^ATT
4332 AATATT
TaqI 1537 TCGA T^CGA
4228 TCGA
TthHB8I 1537 TCGA T^CGA
4228 TCGA
XbaI 2114 TCTAGA T^CTAGA
3794 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI AspHI AvaII BbvCI
BbvI BfaI BmyI BpmI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsmI Bsp1286I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraI DraII EcoNI EcoO109I
EcoRII Fnu4HI FokI HaeIII HinfI
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MvaI MwoI NdeII
NlaIII NlaIV NspI PflMI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
SpeI SphI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XhoII