##################################
# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI Asp718I AspEI
AvaI AviII AvrII BbeI BbrPI
BbvCI BcgI BciVI BfrI BglI
BlnI BsaAI BsaHI BsaWI BseAI
BsiEI BsiWI BsmI BsoBI BspEI
BsrBI BsrFI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I Cfr10I CpoI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRV FseI
FspI Hin1I HincII HindII HpaI
KasI KpnI KspI MroI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NspV PaeR7I PinAI PmaCI
PmeI PmlI PpuMI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I StuI XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccI 93 GTAGAC GT^[AC][GT]AC
ApaI 2739 GGGCCC GGGCC^C
BalI 961 TGGCCA TGG^CCA
BamHI 2044 GGATCC G^GATCC
BanI 1130 GGCACC G^G[CT][GA]CC
BbsI 2339 GTCTTC GAAGAC(2/6)
BlpI 3594 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 3594 GCTAAGC GC^T[ACGT]AGC
BpuAI 2339 GTCTTC GAAGAC(2/6)
BsaBI 3549 GATGATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2877 GAGACC GGTCTC(1/5)
BsmBI 878 CGTCTC CGTCTC(1/5)
BspDI 3989 ATCGAT AT^CGAT
BspMI 4012 ACCTGC ACCTGC(4/8)
BstAPI 2711 GCAACAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 3594 GCTAAGC GC^T[ACGT]AGC
ClaI 3989 ATCGAT AT^CGAT
DraII 2739 GGGCCCT [GA]G^G[ACGT]CC[CT]
EaeI 961 TGGCCA [CT]^GGCC[GA]
EarI 555 CTCTTC CTCTTC(1/4)
EcoO109I 2739 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT22I 619 ATGCAT ATGCA^T
FauI 532 GCGGG CCCGC(4/6)
HaeII 399 AGCGCC [GA]GCGC^[CT]
HindIII 1060 AAGCTT A^AGCTT
Ksp632I 555 CTCTTC CTCTTC(1/4)
MaeII 1819 ACGT A^CGT
MamI 3549 GATGATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 961 TGGCCA TGG^CCA
MscI 961 TGGCCA TGG^CCA
NsiI 619 ATGCAT ATGCA^T
PacI 3714 TTAATTAA TTAAT^TAA
PshAI 452 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1254 CTGCAG CTGCA^G
SwaI 2263 ATTTAAAT ATTT^AAAT
TaqI 3990 TCGA T^CGA
TthHB8I 3990 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AspHI 444 GTGCTC G[AT]GC[AT]^C
1085 GAGCAC
AspI 873 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1386 GACTTTGTC
BclI 782 TGATCA T^GATCA
2252 TGATCA
BcnI 560 CCCGG CC^[GC]GG
567 CCGGG
BglII 3062 AGATCT A^GATCT
3274 AGATCT
BseRI 3365 CTCCTC GAGGAG(10/8)
3406 CTCCTC
BsiHKAI 444 GTGCTC G[AT]GC[AT]^C
1085 GAGCAC
BspHI 2223 TCATGA T^CATGA
2305 TCATGA
BsrDI 1475 CATTGC GCAATG(2/0)
1222 GCAATG
CfoI 390 GCGC GCG^C
400 GCGC
DsaI 743 CCATGG C^C[GA][CT]GG
1246 CCATGG
EcoRI 937 GAATTC G^AATTC
970 GAATTC
FbaI 782 TGATCA T^GATCA
2252 TGATCA
HhaI 390 GCGC GCG^C
400 GCGC
HinP1I 390 GCGC G^CGC
400 GCGC
MfeI 868 CAATTG C^AATTG
3923 CAATTG
MunI 868 CAATTG C^AATTG
3923 CAATTG
NciI 560 CCCGG CC^[GC]GG
567 CCGGG
NcoI 743 CCATGG C^CATGG
1246 CCATGG
PflFI 873 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1386 GACTTTGTC
PleI 1357 GACTC GAGTC(4/5)
1650 GACTC
RcaI 2223 TCATGA T^CATGA
2305 TCATGA
Tth111I 873 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1386 GACTTTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AseI
AsnI Asp700I AvaII BanII BbvI
BfaI BmyI BpmI BsaJI BsgI
BsiYI BslI BsmAI BsmFI Bsp1286I
Bsp1407I BspLU11I BsrGI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
Cfr13I DdeI DpnII DraI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HgaI HinfI HpaII HphI ItaI
MaeI MaeIII MboI MboII MflI
MluI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NdeII
NlaIII NlaIV NspI PflMI PshBI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SspBI SspI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Van91I XbaI XhoII XmnI