##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII Acc65I AccIII AflII AhdI
Alw44I AocI ApaLI AscI Asp718I
AspEI AvrII BamHI BbrPI BbvCI
BfrI BglI BlnI BsaAI BsaBI
BseAI BseRI BsgI BsiWI BspEI
BsrDI BssHII Bst1107I BstZ17I Bsu36I
CpoI DraIII DrdI DsaI Eam1105I
Eco81I EcoNI EcoRI EcoT22I FauI
FseI KpnI KspI MamI MluI
MroI NaeI NcoI NgoMIV NheI
NotI NruI NsiI PacI PmaCI
PmeI PmlI PpuMI PshAI PstI
PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfiI
SgrAI SmaI SnaBI Sse8387I SwaI
XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AccI 2420 GTCTAC GT^[AC][GT]AC
AccII 26 CGCG CG^CG
AclI 1078 AACGTT AA^CGTT
AcyI 32 GGCGCC G[GA]^CG[CT]C
AflIII 1979 ACATGT A^C[GA][CT]GT
AgeI 367 ACCGGT A^CCGGT
ApaI 96 GGGCCC GGGCC^C
AspHI 2092 GTGCTC G[AT]GC[AT]^C
AspI 2073 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2206 TGCGCA TGC^GCA
BbeI 32 GGCGCC GGCGC^C
BbsI 1368 GAAGAC GAAGAC(2/6)
BcgI 806 CGAGACTCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1895 GGATAC GTATCC(6/5)
BglII 1571 AGATCT A^GATCT
BpuAI 1368 GAAGAC GAAGAC(2/6)
BsaHI 32 GGCGCC G[GA]^CG[CT]C
BsaWI 367 ACCGGT [AT]^CCGG[AT]
BsiEI 51 CGGCCG CG[GA][CT]^CG
BsiHKAI 2092 GTGCTC G[AT]GC[AT]^C
BsmBI 764 GAGACG CGTCTC(1/5)
BsmFI 3366 GTCCC GGGAC(10/14)
BsmI 1535 GAATGC GAATGC(1/-1)
BspHI 2855 TCATGA T^CATGA
BspLU11I 1979 ACATGT A^CATGT
BsrBI 3 GAGCGG CCGCTC(-3/-3)
BsrFI 367 ACCGGT [GA]^CCGG[CT]
BstUI 26 CGCG CG^CG
BstXI 3380 CCAGTTTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 367 ACCGGT [GA]^CCGG[CT]
DraII 95 GGGGCCC [GA]G^G[ACGT]CC[CT]
EagI 51 CGGCCG C^GGCCG
EclXI 51 CGGCCG C^GGCCG
Eco52I 51 CGGCCG C^GGCCG
EcoO109I 95 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoRV 912 GATATC GAT^ATC
FspI 2206 TGCGCA TGC^GCA
HgaI 3113 GCGTC GACGC(5/10)
Hin1I 32 GGCGCC G[GA]^CG[CT]C
KasI 32 GGCGCC G^GCGCC
MfeI 160 CAATTG C^AATTG
MunI 160 CAATTG C^AATTG
MvnI 26 CGCG CG^CG
NarI 32 GGCGCC GG^CGCC
NdeI 2838 CATATG CA^TATG
PflFI 2073 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3545 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 367 ACCGGT A^CCGGT
Psp1406I 1078 AACGTT AA^CGTT
RcaI 2855 TCATGA T^CATGA
SexAI 3251 ACCTGGT A^CC[AT]GGT
SfoI 32 GGCGCC GGC^GCC
SpeI 849 ACTAGT A^CTAGT
StuI 3633 AGGCCT AGG^CCT
Tth111I 2073 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3545 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
Aor51HI 486 AGCGCT AGC^GCT
2812 AGCGCT
AseI 1323 ATTAAT AT^TAAT
2226 ATTAAT
AsnI 1323 ATTAAT AT^TAAT
2226 ATTAAT
Asp700I 2357 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3186 GAAGCTATTC
AvaI 1444 CTCGAG C^[CT]CG[GA]G
2584 CTCGAG
BalI 3721 TGGCCA TGG^CCA
3848 TGGCCA
BanI 32 GGCGCC G^G[CT][GA]CC
414 GGTGCC
BanII 96 GGGCCC G[GA]GC[CT]^C
769 GAGCCC
BsaI 2271 GAGACC GGTCTC(1/5)
78 GGTCTC
BsoBI 1444 CTCGAG C^[CT]CG[GA]G
2584 CTCGAG
BspDI 378 ATCGAT AT^CGAT
3143 ATCGAT
BssSI 319 CTCGTG CACGAG(-5/-1)
811 CTCGTG
BstEII 370 GGTTACC G^GT[ACGT]ACC
2070 GGTGACC
BstPI 370 GGTTACC G^GT[ACGT]ACC
2070 GGTGACC
ClaI 378 ATCGAT AT^CGAT
3143 ATCGAT
Eco47III 486 AGCGCT AGC^GCT
2812 AGCGCT
EcoO65I 370 GGTTACC G^GT[ACGT]ACC
2070 GGTGACC
EcoT14I 1033 CCAAGG C^C[AT][AT]GG
3469 CCTTGG
HindIII 1969 AAGCTT A^AGCTT
2402 AAGCTT
HpaI 3051 GTTAAC GTT^AAC
3197 GTTAAC
MaeII 1079 ACGT A^CGT
2378 ACGT
MluNI 3721 TGGCCA TGG^CCA
3848 TGGCCA
MscI 3721 TGGCCA TGG^CCA
3848 TGGCCA
PaeR7I 1444 CTCGAG C^TCGAG
2584 CTCGAG
PshBI 1323 ATTAAT AT^TAAT
2226 ATTAAT
PvuII 2028 CAGCTG CAG^CTG
3652 CAGCTG
SphI 2408 GCATGC GCATG^C
2896 GCATGC
StyI 1033 CCAAGG C^C[AT][AT]GG
3469 CCTTGG
XbaI 713 TCTAGA T^CTAGA
2298 TCTAGA
XhoI 1444 CTCGAG C^TCGAG
2584 CTCGAG
XmnI 2357 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3186 GAAGCTATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AvaII BbvI BclI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BsaJI BsiYI BslI BsmAI
Bsp1286I Bsp1407I BspMI BsrGI BsrI
BssKI BstAPI BstBI BstF5I BstNI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraI EaeI EarI
EcoRII FbaI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI NspV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfuI SmlI SspBI SspI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII