##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII Acc65I AclI AcyI AgeI
Alw44I ApaI ApaLI AscI AseI
AsnI Asp718I AviII AvrII BamHI
BbeI BbrPI BlnI BlpI Bpu1102I
BsaHI BsiEI BsiWI Bsp1407I BspDI
BspLU11I BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I CelII ClaI
CpoI DraIII EagI EclXI Eco52I
EcoO65I EcoRV EcoT22I FseI FspI
Hin1I HindIII HpaI KasI KpnI
NarI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PvuI RsrII SbfI SfiI
SfoI SfuI SgrAI SnaBI Sse8387I
SspBI StuI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 251 GTCGAC GT^[AC][GT]AC
AccIII 1002 TCCGGA T^CCGGA
AflII 2997 CTTAAG C^TTAAG
AflIII 1559 ACGCGT A^C[GA][CT]GT
Aor51HI 471 AGCGCT AGC^GCT
AspHI 95 GAGCTC G[AT]GC[AT]^C
BcgI 3874 GCATGGACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 594 GTATCC GTATCC(6/5)
BclI 1796 TGATCA T^GATCA
BfrI 2997 CTTAAG C^TTAAG
BglII 3568 AGATCT A^GATCT
BsaAI 3621 TACGTG [CT]AC^GT[GA]
BsaBI 4076 GATAGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1002 TCCGGA T^CCGGA
BsiHKAI 95 GAGCTC G[AT]GC[AT]^C
BspEI 1002 TCCGGA T^CCGGA
BspHI 741 TCATGA T^CATGA
BstAPI 3602 GCATGGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DrdI 879 GACTCCCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 471 AGCGCT AGC^GCT
EcoNI 325 CCTGTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 3841 GAATTC G^AATTC
FbaI 1796 TGATCA T^GATCA
HaeII 471 AGCGCT [GA]GCGC^[CT]
KspI 1483 CCGCGG CCGC^GG
MamI 4076 GATAGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1737 CAATTG C^AATTG
MluI 1559 ACGCGT A^CGCGT
MroI 1002 TCCGGA T^CCGGA
MunI 1737 CAATTG C^AATTG
NaeI 1408 GCCGGC GCC^GGC
NgoMIV 1408 GCCGGC G^CCGGC
PshAI 686 GACATGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1773 CTGCAG CTGCA^G
RcaI 741 TCATGA T^CATGA
SacI 95 GAGCTC GAGCT^C
SacII 1483 CCGCGG CCGC^GG
SalI 251 GTCGAC G^TCGAC
SapI 1274 GAAGAGC GCTCTTC(1/4)
ScaI 3616 AGTACT AGT^ACT
SexAI 3688 ACCAGGT A^CC[AT]GGT
SpeI 3743 ACTAGT A^CTAGT
SspI 4053 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AhdI 3159 GACCTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4136 GACCGTCTGTC
AlwNI 129 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
396 CAGTTACTG
AocI 299 CCTCAGG CC^T[ACGT]AGG
3918 CCTGAGG
Asp700I 707 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1072 GAACCTCTTC
AspEI 3159 GACCTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4136 GACCGTCTGTC
AvaII 1511 GGACC G^G[AT]CC
3198 GGTCC
BalI 372 TGGCCA TGG^CCA
3949 TGGCCA
BglI 899 GCCAAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3961 GCCGGTTTGGC
BsaWI 1002 TCCGGA [AT]^CCGG[AT]
3649 ACCGGA
BsmBI 1132 CGTCTC CGTCTC(1/5)
3585 GAGACG
BsrBI 1030 GAGCGG CCGCTC(-3/-3)
3885 GAGCGG
BsrFI 1408 GCCGGC [GA]^CCGG[CT]
3961 GCCGGT
BssSI 728 CACGAG CACGAG(-5/-1)
3064 CACGAG
Bsu36I 299 CCTCAGG CC^T[ACGT]AGG
3918 CCTGAGG
Cfr10I 1408 GCCGGC [GA]^CCGG[CT]
3961 GCCGGT
DraI 2656 TTTAAA TTT^AAA
3822 TTTAAA
Eam1105I 3159 GACCTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4136 GACCGTCTGTC
Eco81I 299 CCTCAGG CC^T[ACGT]AGG
3918 CCTGAGG
HgaI 3596 GACGC GACGC(5/10)
691 GCGTC
HincII 251 GTCGAC GT[CT]^[GA]AC
3593 GTTGAC
HindII 251 GTCGAC GT[CT]^[GA]AC
3593 GTTGAC
MluNI 372 TGGCCA TGG^CCA
3949 TGGCCA
MscI 372 TGGCCA TGG^CCA
3949 TGGCCA
SmaI 2068 CCCGGG CCC^GGG
2368 CCCGGG
SphI 2014 GCATGC GCATG^C
2314 GCATGC
XcmI 306 CCATTCTGGGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
336 CCAAGTCGGGCATGG
XmaCI 2068 CCCGGG C^CCGGG
2368 CCCGGG
XmaI 2068 CCCGGG C^CCGGG
2368 CCCGGG
XmnI 707 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1072 GAACCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AspI AvaI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflFI PflMI
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XhoII