##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
Alw44I ApaLI AseI AsnI Asp700I
Asp718I AviII BbrPI BcgI BfrI
BsaBI BseAI BsiWI BsmI BspDI
BspEI BspHI BsrDI BssSI Bst1107I
BstBI BstZ17I ClaI CpoI DrdI
EagI EclXI Eco52I EcoT22I FseI
FspI HincII HindII HindIII HpaI
KpnI KspI MamI MfeI MluI
MroI MunI NdeI NheI NotI
NsiI NspV PacI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI ScaI
SfiI SfuI SgrAI SnaBI StuI
SwaI XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 2531 GACGTC GACGT^C
AccI 3763 GTCTAC GT^[AC][GT]AC
AflIII 1251 ACATGT A^C[GA][CT]GT
AhdI 3287 GACTGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2188 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1440 AGCGCT AGC^GCT
AscI 32 GGCGCGCC GG^CGCGCC
AspEI 3287 GACTGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2342 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3266 CCTAGG C^CTAGG
BamHI 1919 GGATCC G^GATCC
BciVI 1843 GGATAC GTATCC(6/5)
BclI 2232 TGATCA T^GATCA
BglII 903 AGATCT A^GATCT
BlnI 3266 CCTAGG C^CTAGG
BsaAI 2099 TACGTG [CT]AC^GT[GA]
BsaWI 3372 ACCGGA [AT]^CCGG[AT]
BsiEI 1745 CGACCG CG[GA][CT]^CG
BsmBI 2347 CGTCTC CGTCTC(1/5)
BspLU11I 1251 ACATGT A^CATGT
Bsu36I 2188 CCTCAGG CC^T[ACGT]AGG
Eam1105I 3287 GACTGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1440 AGCGCT AGC^GCT
Eco81I 2188 CCTCAGG CC^T[ACGT]AGG
EcoRI 4104 GAATTC G^AATTC
EcoRV 2618 GATATC GAT^ATC
FbaI 2232 TGATCA T^GATCA
HgaI 415 GACGC GACGC(5/10)
NcoI 3471 CCATGG C^CATGG
PflFI 2342 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
SapI 2215 GCTCTTC GCTCTTC(1/4)
SbfI 2320 CCTGCAGG CCTGCA^GG
SpeI 3258 ACTAGT A^CTAGT
SphI 2422 GCATGC GCATG^C
Sse8387I 2320 CCTGCAGG CCTGCA^GG
SspI 4151 AATATT AAT^ATT
Tth111I 2342 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4144 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcsI 4104 GAATTC [GA]^AATT[CT]
4257 AAATTT
ApaI 55 GGGCCC GGGCC^C
1411 GGGCCC
ApoI 4104 GAATTC [GA]^AATT[CT]
4257 AAATTT
BalI 1779 TGGCCA TGG^CCA
3770 TGGCCA
BbeI 856 GGCGCC GGCGC^C
1014 GGCGCC
BbvCI 798 CCTCAGC CCTCAGC(-5/-2)
1303 CCTCAGC
BlpI 1575 GCTCAGC GC^T[ACGT]AGC
2913 GCTGAGC
Bpu1102I 1575 GCTCAGC GC^T[ACGT]AGC
2913 GCTGAGC
Bsp1407I 2271 TGTACA T^GTACA
3426 TGTACA
BsrGI 2271 TGTACA T^GTACA
3426 TGTACA
BssHII 15 GCGCGC G^CGCGC
33 GCGCGC
BstEII 2072 GGTGACC G^GT[ACGT]ACC
3660 GGTCACC
BstPI 2072 GGTGACC G^GT[ACGT]ACC
3660 GGTCACC
BstYI 903 AGATCT [GA]^GATC[CT]
1919 GGATCC
CelII 1575 GCTCAGC GC^T[ACGT]AGC
2913 GCTGAGC
DraI 3241 TTTAAA TTT^AAA
4290 TTTAAA
DraIII 2383 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
3354 CACGGGGTG
EcoNI 1966 CCTGCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3460 CCTGCTCCAGG
EcoO65I 2072 GGTGACC G^GT[ACGT]ACC
3660 GGTCACC
KasI 856 GGCGCC G^GCGCC
1014 GGCGCC
MflI 903 AGATCT [GA]^GATC[CT]
1919 GGATCC
MluNI 1779 TGGCCA TGG^CCA
3770 TGGCCA
MscI 1779 TGGCCA TGG^CCA
3770 TGGCCA
NarI 856 GGCGCC GG^CGCC
1014 GGCGCC
NruI 837 TCGCGA TCG^CGA
1040 TCGCGA
PshAI 545 GACTGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4047 GACTGCTGTC
SexAI 2283 ACCTGGT A^CC[AT]GGT
3914 ACCAGGT
SfoI 856 GGCGCC GGC^GCC
1014 GGCGCC
SspBI 2271 TGTACA T^GTACA
3426 TGTACA
XhoII 903 AGATCT [GA]^GATC[CT]
1919 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaI AvaII
BanI BanII BbsI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV NspI PaeR7I
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoI XmaCI XmaI