##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AgeI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI AspI
AviII BalI BamHI BbeI BbrPI
BglI BsaAI BsaBI BsaHI BseAI
BsiEI BsiWI BspDI BspEI BsrBI
BsrFI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstZ17I Cfr10I
ClaI CpoI DraII EagI EclXI
Eco47III Eco52I EcoNI EcoO109I EcoO65I
EcoRV FseI FspI Hin1I KasI
KspI MamI MluI MluNI MroI
MscI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI SfiI SfoI SfuI SgrAI
SnaBI Sse8387I Tth111I Van91I XcmI
XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AflII 1139 CTTAAG C^TTAAG
AflIII 4713 ACATGT A^C[GA][CT]GT
AhdI 4499 GACTATCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 4499 GACTATCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 3533 CCCGGG C^[CT]CG[GA]G
AvaII 1242 GGTCC G^G[AT]CC
AvrII 458 CCTAGG C^CTAGG
BcgI 242 GCAGGAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1139 CTTAAG C^TTAAG
BglII 2034 AGATCT A^GATCT
BlnI 458 CCTAGG C^CTAGG
BlpI 702 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 702 GCTCAGC GC^T[ACGT]AGC
BsgI 1082 GTGCAG GTGCAG(16/14)
BsoBI 3533 CCCGGG C^[CT]CG[GA]G
BspHI 1688 TCATGA T^CATGA
BspLU11I 4713 ACATGT A^CATGT
BstAPI 1585 GCATGTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 702 GCTCAGC GC^T[ACGT]AGC
Cfr13I 1242 GGTCC G^G[ACGT]CC
DraIII 2999 CACTAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3796 GACCTCCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 4061 CGGCCA [CT]^GGCC[GA]
Eam1105I 4499 GACTATCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 2043 ATGCAT ATGCA^T
HaeII 3330 AGCGCC [GA]GCGC^[CT]
HindIII 4173 AAGCTT A^AGCTT
HpaI 2520 GTTAAC GTT^AAC
MaeII 3731 ACGT A^CGT
NsiI 2043 ATGCAT ATGCA^T
PvuII 1678 CAGCTG CAG^CTG
RcaI 1688 TCATGA T^CATGA
SapI 510 GAAGAGC GCTCTTC(1/4)
Sau96I 1242 GGTCC G^G[ACGT]CC
SexAI 4451 ACCTGGT A^CC[AT]GGT
SmaI 3533 CCCGGG CCC^GGG
SpeI 4395 ACTAGT A^CTAGT
SphI 207 GCATGC GCATG^C
SspI 1884 AATATT AAT^ATT
StuI 4217 AGGCCT AGG^CCT
SwaI 2603 ATTTAAAT ATTT^AAAT
XmaCI 3533 CCCGGG C^CCGGG
XmaI 3533 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
BciVI 1458 GGATAC GTATCC(6/5)
2014 GTATCC
BclI 2490 TGATCA T^GATCA
2664 TGATCA
BsaWI 273 ACCGGA [AT]^CCGG[AT]
3951 TCCGGT
BsmBI 167 CGTCTC CGTCTC(1/5)
339 CGTCTC
Bsp1407I 4536 TGTACA T^GTACA
4797 TGTACA
BspMI 1289 GCAGGT ACCTGC(4/8)
1906 GCAGGT
BsrDI 1604 GCAATG GCAATG(2/0)
4013 GCAATG
BsrGI 4536 TGTACA T^GTACA
4797 TGTACA
DsaI 302 CCATGG C^C[GA][CT]GG
405 CCATGG
FbaI 2490 TGATCA T^GATCA
2664 TGATCA
HgaI 3643 GACGC GACGC(5/10)
3834 GACGC
HincII 2520 GTTAAC GT[CT]^[GA]AC
3199 GTCAAC
HindII 2520 GTTAAC GT[CT]^[GA]AC
3199 GTCAAC
MfeI 609 CAATTG C^AATTG
2796 CAATTG
MunI 609 CAATTG C^AATTG
2796 CAATTG
NcoI 302 CCATGG C^CATGG
405 CCATGG
PshAI 3013 GACAAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4045 GACACCAGTC
PstI 1904 CTGCAG CTGCA^G
4040 CTGCAG
ScaI 2597 AGTACT AGT^ACT
4280 AGTACT
SspBI 4536 TGTACA T^GTACA
4797 TGTACA
XbaI 2159 TCTAGA T^CTAGA
3654 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
Acc65I AciI AcsI AfaI AluI
AlwI AlwNI AocI ApoI Asp700I
Asp718I AspHI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BsrI BssKI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CfoI DdeI DpnII DraI EarI
Eco81I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
KpnI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
RsaI SacI Sau3AI ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI