################################## # NO CUT ENZYMES ( 51 enzymes) # ################################## AatII Acc65I AclI AflII AgeI AscI AseI AsnI Asp718I AvrII BciVI BclI BfaI BfrI BlnI BsaBI BspDI BssHII Bst1107I BstZ17I ClaI CpoI DraI EcoRI EcoT22I FbaI FseI HindIII HpaI KpnI MaeI MamI NdeI NheI NruI NsiI PacI PinAI PmeI PshBI Psp1406I RsrII SbfI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI XbaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccIII 3433 TCCGGA T^CCGGA AviII 3611 TGCGCA TGC^GCA BamHI 2186 GGATCC G^GATCC BanI 3038 GGCGCC G^G[CT][GA]CC BbeI 3038 GGCGCC GGCGC^C BbrPI 3884 CACGTG CAC^GTG BbvCI 2920 CCTCAGC CCTCAGC(-5/-2) BsaI 1093 GGTCTC GGTCTC(1/5) BsaWI 3433 TCCGGA [AT]^CCGG[AT] BseAI 3433 TCCGGA T^CCGGA BsiWI 3504 CGTACG C^GTACG BsmI 3757 GCATTC GAATGC(1/-1) BspEI 3433 TCCGGA T^CCGGA BspHI 1431 TCATGA T^CATGA BsrDI 3256 CATTGC GCAATG(2/0) BssSI 343 CACGAG CACGAG(-5/-1) BstBI 495 TTCGAA TT^CGAA EcoRV 422 GATATC GAT^ATC FspI 3611 TGCGCA TGC^GCA HincII 2330 GTCGAC GT[CT]^[GA]AC HindII 2330 GTCGAC GT[CT]^[GA]AC KasI 3038 GGCGCC G^GCGCC KspI 1281 CCGCGG CCGC^GG MfeI 3828 CAATTG C^AATTG MluI 1755 ACGCGT A^CGCGT MroI 3433 TCCGGA T^CCGGA MunI 3828 CAATTG C^AATTG NarI 3038 GGCGCC GG^CGCC NcoI 1850 CCATGG C^CATGG NotI 638 GCGGCCGC GC^GGCCGC NspV 495 TTCGAA TT^CGAA PflMI 3624 CCACAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 3884 CACGTG CAC^GTG PmlI 3884 CACGTG CAC^GTG PvuI 405 CGATCG CGAT^CG RcaI 1431 TCATGA T^CATGA SacII 1281 CCGCGG CCGC^GG SalI 2330 GTCGAC G^TCGAC SapI 841 GCTCTTC GCTCTTC(1/4) ScaI 513 AGTACT AGT^ACT SexAI 3423 ACCTGGT A^CC[AT]GGT SfiI 2386 GGCCTGCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 3038 GGCGCC GGC^GCC SfuI 495 TTCGAA TT^CGAA SmaI 3713 CCCGGG CCC^GGG Van91I 3624 CCACAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 3713 CCCGGG C^CCGGG XmaI 3713 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AhdI 1919 GACATTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3545 GACGCCTGGTC AocI 2441 CCTGAGG CC^T[ACGT]AGG 3053 CCTCAGG AspEI 1919 GACATTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3545 GACGCCTGGTC AspI 2324 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 2878 GACCAAGTC BbsI 2321 GAAGAC GAAGAC(2/6) 548 GTCTTC BcgI 3484 CGACAAGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 3013 GCAGACAAGTCG BglII 138 AGATCT A^GATCT 3199 AGATCT BlpI 2419 GCTCAGC GC^T[ACGT]AGC 2476 GCTCAGC Bpu1102I 2419 GCTCAGC GC^T[ACGT]AGC 2476 GCTCAGC BpuAI 2321 GAAGAC GAAGAC(2/6) 548 GTCTTC BsaAI 1493 TACGTG [CT]AC^GT[GA] 3884 CACGTG BspLU11I 1299 ACATGT A^CATGT 1362 ACATGT BstEII 905 GGTGACC G^GT[ACGT]ACC 3717 GGTCACC BstPI 905 GGTGACC G^GT[ACGT]ACC 3717 GGTCACC Bsu36I 2441 CCTGAGG CC^T[ACGT]AGG 3053 CCTCAGG CelII 2419 GCTCAGC GC^T[ACGT]AGC 2476 GCTCAGC DraIII 3371 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG 3884 CACGTGGTG DrdI 2728 GACAGCCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2782 GACTGCAGCGTC Eam1105I 1919 GACATTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3545 GACGCCTGGTC Eco81I 2441 CCTGAGG CC^T[ACGT]AGG 3053 CCTCAGG EcoO65I 905 GGTGACC G^GT[ACGT]ACC 3717 GGTCACC NaeI 1647 GCCGGC GCC^GGC 2848 GCCGGC NgoMIV 1647 GCCGGC G^CCGGC 2848 GCCGGC PaeR7I 105 CTCGAG C^TCGAG 1861 CTCGAG PflFI 2324 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 2878 GACCAAGTC PshAI 2048 GACGCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 3647 GACCTGGGTC SacI 59 GAGCTC GAGCT^C 677 GAGCTC Tth111I 2324 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 2878 GACCAAGTC XhoI 105 CTCGAG C^TCGAG 1861 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 130 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AflIII AluI Alw44I AlwI AlwNI Aor51HI ApaI ApaLI ApoI Asp700I AspHI AvaI AvaII BalI BanII BbvI BcnI BglI BmyI BpmI BsaHI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrBI BsrFI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EarI EclXI Eco47III Eco52I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmnI