##################################
# NO CUT ENZYMES ( 51 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AscI AseI AsnI Asp718I AvrII
BciVI BclI BfaI BfrI BlnI
BsaBI BspDI BssHII Bst1107I BstZ17I
ClaI CpoI DraI EcoRI EcoT22I
FbaI FseI HindIII HpaI KpnI
MaeI MamI NdeI NheI NruI
NsiI PacI PinAI PmeI PshBI
Psp1406I RsrII SbfI SgrAI SnaBI
SpeI SphI Sse8387I SspI SwaI
XbaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccIII 3433 TCCGGA T^CCGGA
AviII 3611 TGCGCA TGC^GCA
BamHI 2186 GGATCC G^GATCC
BanI 3038 GGCGCC G^G[CT][GA]CC
BbeI 3038 GGCGCC GGCGC^C
BbrPI 3884 CACGTG CAC^GTG
BbvCI 2920 CCTCAGC CCTCAGC(-5/-2)
BsaI 1093 GGTCTC GGTCTC(1/5)
BsaWI 3433 TCCGGA [AT]^CCGG[AT]
BseAI 3433 TCCGGA T^CCGGA
BsiWI 3504 CGTACG C^GTACG
BsmI 3757 GCATTC GAATGC(1/-1)
BspEI 3433 TCCGGA T^CCGGA
BspHI 1431 TCATGA T^CATGA
BsrDI 3256 CATTGC GCAATG(2/0)
BssSI 343 CACGAG CACGAG(-5/-1)
BstBI 495 TTCGAA TT^CGAA
EcoRV 422 GATATC GAT^ATC
FspI 3611 TGCGCA TGC^GCA
HincII 2330 GTCGAC GT[CT]^[GA]AC
HindII 2330 GTCGAC GT[CT]^[GA]AC
KasI 3038 GGCGCC G^GCGCC
KspI 1281 CCGCGG CCGC^GG
MfeI 3828 CAATTG C^AATTG
MluI 1755 ACGCGT A^CGCGT
MroI 3433 TCCGGA T^CCGGA
MunI 3828 CAATTG C^AATTG
NarI 3038 GGCGCC GG^CGCC
NcoI 1850 CCATGG C^CATGG
NotI 638 GCGGCCGC GC^GGCCGC
NspV 495 TTCGAA TT^CGAA
PflMI 3624 CCACAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 3884 CACGTG CAC^GTG
PmlI 3884 CACGTG CAC^GTG
PvuI 405 CGATCG CGAT^CG
RcaI 1431 TCATGA T^CATGA
SacII 1281 CCGCGG CCGC^GG
SalI 2330 GTCGAC G^TCGAC
SapI 841 GCTCTTC GCTCTTC(1/4)
ScaI 513 AGTACT AGT^ACT
SexAI 3423 ACCTGGT A^CC[AT]GGT
SfiI 2386 GGCCTGCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 3038 GGCGCC GGC^GCC
SfuI 495 TTCGAA TT^CGAA
SmaI 3713 CCCGGG CCC^GGG
Van91I 3624 CCACAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 3713 CCCGGG C^CCGGG
XmaI 3713 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AhdI 1919 GACATTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3545 GACGCCTGGTC
AocI 2441 CCTGAGG CC^T[ACGT]AGG
3053 CCTCAGG
AspEI 1919 GACATTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3545 GACGCCTGGTC
AspI 2324 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2878 GACCAAGTC
BbsI 2321 GAAGAC GAAGAC(2/6)
548 GTCTTC
BcgI 3484 CGACAAGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3013 GCAGACAAGTCG
BglII 138 AGATCT A^GATCT
3199 AGATCT
BlpI 2419 GCTCAGC GC^T[ACGT]AGC
2476 GCTCAGC
Bpu1102I 2419 GCTCAGC GC^T[ACGT]AGC
2476 GCTCAGC
BpuAI 2321 GAAGAC GAAGAC(2/6)
548 GTCTTC
BsaAI 1493 TACGTG [CT]AC^GT[GA]
3884 CACGTG
BspLU11I 1299 ACATGT A^CATGT
1362 ACATGT
BstEII 905 GGTGACC G^GT[ACGT]ACC
3717 GGTCACC
BstPI 905 GGTGACC G^GT[ACGT]ACC
3717 GGTCACC
Bsu36I 2441 CCTGAGG CC^T[ACGT]AGG
3053 CCTCAGG
CelII 2419 GCTCAGC GC^T[ACGT]AGC
2476 GCTCAGC
DraIII 3371 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
3884 CACGTGGTG
DrdI 2728 GACAGCCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2782 GACTGCAGCGTC
Eam1105I 1919 GACATTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3545 GACGCCTGGTC
Eco81I 2441 CCTGAGG CC^T[ACGT]AGG
3053 CCTCAGG
EcoO65I 905 GGTGACC G^GT[ACGT]ACC
3717 GGTCACC
NaeI 1647 GCCGGC GCC^GGC
2848 GCCGGC
NgoMIV 1647 GCCGGC G^CCGGC
2848 GCCGGC
PaeR7I 105 CTCGAG C^TCGAG
1861 CTCGAG
PflFI 2324 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2878 GACCAAGTC
PshAI 2048 GACGCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3647 GACCTGGGTC
SacI 59 GAGCTC GAGCT^C
677 GAGCTC
Tth111I 2324 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2878 GACCAAGTC
XhoI 105 CTCGAG C^TCGAG
1861 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI Alw44I AlwI
AlwNI Aor51HI ApaI ApaLI ApoI
Asp700I AspHI AvaI AvaII BalI
BanII BbvI BcnI BglI BmyI
BpmI BsaHI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I Bsp1407I
BspMI BsrBI BsrFI BsrGI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI EarI EclXI Eco47III
Eco52I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII XmnI