##################################
# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflIII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspHI AspI AvaI AvrII BamHI
BanI BbeI BbrPI BcgI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaWI BseAI BsiHKAI BsiWI BsmBI
BsoBI BspEI BspLU11I BsrBI BssHII
BssSI Bst1107I BstBI BstZ17I CelII
CpoI DrdI Eam1105I Eco47III EcoRI
EcoRV FseI HaeII HgaI Hin1I
KasI KpnI KspI MamI MluI
MroI MspA1I NarI NdeI NheI
NotI NruI NspV PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI PvuII RsrII SacI
SacII SapI SbfI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SwaI Tth111I XbaI
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AlwI 2105 GGATC GGATC(4/5)
AocI 4116 CCTTAGG CC^T[ACGT]AGG
AseI 2763 ATTAAT AT^TAAT
AsnI 2763 ATTAAT AT^TAAT
Asp700I 4138 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 477 TGCGCA TGC^GCA
BciVI 869 GGATAC GTATCC(6/5)
BclI 1473 TGATCA T^GATCA
BglI 889 GCCACAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsiEI 14 CGGCCG CG[GA][CT]^CG
Bsp1407I 4354 TGTACA T^GTACA
BspDI 3317 ATCGAT AT^CGAT
BspHI 4445 TCATGA T^CATGA
BsrGI 4354 TGTACA T^GTACA
BstEII 1415 GGTCACC G^GT[ACGT]ACC
BstPI 1415 GGTCACC G^GT[ACGT]ACC
Bsu36I 4116 CCTTAGG CC^T[ACGT]AGG
ClaI 3317 ATCGAT AT^CGAT
DraIII 3945 CACATAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 14 CGGCCG C^GGCCG
EarI 874 CTCTTC CTCTTC(1/4)
EclXI 14 CGGCCG C^GGCCG
Eco52I 14 CGGCCG C^GGCCG
Eco81I 4116 CCTTAGG CC^T[ACGT]AGG
EcoNI 3675 CCTGGCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1415 GGTCACC G^GT[ACGT]ACC
EcoT22I 1684 ATGCAT ATGCA^T
FauI 45 GCGGG CCCGC(4/6)
FbaI 1473 TGATCA T^GATCA
FspI 477 TGCGCA TGC^GCA
HpaI 562 GTTAAC GTT^AAC
Ksp632I 874 CTCTTC CTCTTC(1/4)
NcoI 5 CCATGG C^CATGG
NsiI 1684 ATGCAT ATGCA^T
PacI 2416 TTAATTAA TTAAT^TAA
PshBI 2763 ATTAAT AT^TAAT
RcaI 4445 TCATGA T^CATGA
SalI 1810 GTCGAC G^TCGAC
SexAI 1068 ACCAGGT A^CC[AT]GGT
SspBI 4354 TGTACA T^GTACA
StuI 1649 AGGCCT AGG^CCT
XmnI 4138 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 44 CGCG CG^CG
61 CGCG
AciI 1420 CCGC CCGC(-3/-1)
45 GCGG
AflII 2667 CTTAAG C^TTAAG
3912 CTTAAG
BalI 8 TGGCCA TGG^CCA
3578 TGGCCA
BanII 1338 GAGCCC G[GA]GC[CT]^C
4174 GAGCCC
BcnI 1853 CCCGG CC^[GC]GG
3355 CCGGG
BfrI 2667 CTTAAG C^TTAAG
3912 CTTAAG
BmyI 1338 GAGCCC G[AGT]GC[ACT]^C
4174 GAGCCC
Bsp1286I 1338 GAGCCC G[AGT]GC[ACT]^C
4174 GAGCCC
BsrDI 373 GCAATG GCAATG(2/0)
3872 GCAATG
BsrFI 23 GCCGGC [GA]^CCGG[CT]
34 GCCGGC
BstAPI 345 GCAACTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3377 GCAGTACATGC
BstUI 44 CGCG CG^CG
61 CGCG
Cfr10I 23 GCCGGC [GA]^CCGG[CT]
34 GCCGGC
DsaI 5 CCATGG C^C[GA][CT]GG
11 CCACGG
EcoT14I 5 CCATGG C^C[AT][AT]GG
4409 CCTTGG
MluNI 8 TGGCCA TGG^CCA
3578 TGGCCA
MscI 8 TGGCCA TGG^CCA
3578 TGGCCA
MvnI 44 CGCG CG^CG
61 CGCG
NaeI 23 GCCGGC GCC^GGC
34 GCCGGC
NciI 1853 CCCGG CC^[GC]GG
3355 CCGGG
NgoMIV 23 GCCGGC G^CCGGC
34 GCCGGC
NspI 3382 ACATGC [GA]CATG^[CT]
4269 GCATGT
PflMI 719 CCAAAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4057 CCATCTGTTGG
PleI 1134 GACTC GAGTC(4/5)
3702 GACTC
PstI 401 CTGCAG CTGCA^G
1982 CTGCAG
ScaI 1817 AGTACT AGT^ACT
3261 AGTACT
StyI 5 CCATGG C^C[AT][AT]GG
4409 CCTTGG
Van91I 719 CCAAAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4057 CCATCTGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccI AcsI AfaI AluI AlwNI
ApoI AvaII BbsI BbvCI BbvI
BfaI BglII BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI BsmI BspMI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII EaeI EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MfeI MflI
MnlI MseI MslI MspI MunI
MvaI MwoI NdeII NlaIII NlaIV
PpuMI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII