##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII Acc65I AccIII AflII Alw44I
ApaLI AscI AseI AsnI Asp718I
AspI AvrII BamHI BbrPI BcgI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaI BseAI BsmBI
Bsp1407I BspDI BspEI BspLU11I BsrGI
BssSI BstBI BstEII BstPI CelII
ClaI CpoI DraIII EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HindIII HpaI KpnI MluI MroI
NcoI NotI NruI NsiI NspV
PacI PaeR7I PflFI PmaCI PmeI
PmlI PshAI PshBI PstI PvuI
RsrII SalI SapI SbfI ScaI
SexAI SfuI SnaBI SpeI SphI
Sse8387I SspBI SwaI Tth111I XcmI
XhoI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AclI 2308 AACGTT AA^CGTT
AgeI 738 ACCGGT A^CCGGT
AhdI 347 GACATCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 601 CCTCAGG CC^T[ACGT]AGG
Aor51HI 685 AGCGCT AGC^GCT
ApaI 1103 GGGCCC GGGCC^C
Asp700I 1448 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 347 GACATCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 582 GAGCTC G[AT]GC[AT]^C
AviII 3051 TGCGCA TGC^GCA
BbsI 2255 GTCTTC GAAGAC(2/6)
BbvCI 319 GCTGAGG CCTCAGC(-5/-2)
BpuAI 2255 GTCTTC GAAGAC(2/6)
BsaAI 411 CACGTA [CT]AC^GT[GA]
BsaBI 1385 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 582 GAGCTC G[AT]GC[AT]^C
BsiWI 1930 CGTACG C^GTACG
BsmI 2703 GCATTC GAATGC(1/-1)
BspMI 2035 ACCTGC ACCTGC(4/8)
BsrDI 2456 GCAATG GCAATG(2/0)
BssHII 1680 GCGCGC G^CGCGC
BstAPI 1112 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 22 CCAAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 601 CCTCAGG CC^T[ACGT]AGG
Eam1105I 347 GACATCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 402 GAAGAG CTCTTC(1/4)
Eco47III 685 AGCGCT AGC^GCT
Eco81I 601 CCTCAGG CC^T[ACGT]AGG
EcoT14I 53 CCAAGG C^C[AT][AT]GG
FspI 3051 TGCGCA TGC^GCA
Ksp632I 402 GAAGAG CTCTTC(1/4)
KspI 564 CCGCGG CCGC^GG
MamI 1385 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2887 CAATTG C^AATTG
MunI 2887 CAATTG C^AATTG
NdeI 3122 CATATG CA^TATG
NheI 1961 GCTAGC G^CTAGC
NspI 421 GCATGT [GA]CATG^[CT]
PflMI 2796 CCAAAGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 738 ACCGGT A^CCGGT
PpuMI 2004 GGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 2308 AACGTT AA^CGTT
PvuII 1113 CAGCTG CAG^CTG
SacI 582 GAGCTC GAGCT^C
SacII 564 CCGCGG CCGC^GG
SfcI 1832 CTACAG C^T[GA][CT]AG
SfiI 1655 GGCCAAGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 1256 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 1556 CCCGGG CCC^GGG
SmlI 2639 CTTGAG C^T[CT][GA]AG
SspI 267 AATATT AAT^ATT
StuI 598 AGGCCT AGG^CCT
StyI 53 CCAAGG C^C[AT][AT]GG
Van91I 2796 CCAAAGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2809 TCTAGA T^CTAGA
XmaCI 1556 CCCGGG C^CCGGG
XmaI 1556 CCCGGG C^CCGGG
XmnI 1448 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 108 GTATAC GT^[AC][GT]AC
2826 GTATAC
AflIII 410 ACACGT A^C[GA][CT]GT
1600 ACGTGT
AlwNI 1110 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1113 CAGCTGCTG
BalI 1387 TGGCCA TGG^CCA
2736 TGGCCA
BpmI 326 CTGGAG CTGGAG(16/14)
379 CTGGAG
BsaWI 208 ACCGGA [AT]^CCGG[AT]
738 ACCGGT
BsgI 1758 CTGCAC GTGCAG(16/14)
2594 GTGCAG
BsmAI 405 GAGAC GTCTC(1/5)
2406 GTCTC
BspHI 1381 TCATGA T^CATGA
1444 TCATGA
Bst1107I 108 GTATAC GTA^TAC
2826 GTATAC
BstYI 41 AGATCC [GA]^GATC[CT]
1924 AGATCC
BstZ17I 108 GTATAC GTA^TAC
2826 GTATAC
DrdI 1905 GACCGGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2204 GACGGGACTGTC
MflI 41 AGATCC [GA]^GATC[CT]
1924 AGATCC
MluNI 1387 TGGCCA TGG^CCA
2736 TGGCCA
MscI 1387 TGGCCA TGG^CCA
2736 TGGCCA
NaeI 1257 GCCGGC GCC^GGC
1719 GCCGGC
NgoMIV 1257 GCCGGC G^CCGGC
1719 GCCGGC
RcaI 1381 TCATGA T^CATGA
1444 TCATGA
TfiI 607 GAATC G^A[AT]TC
2892 GAATC
TspRI 691 CAGTG CA[GC]TG[ACGT][ACGT]^
2876 CACTG
XhoII 41 AGATCC [GA]^GATC[CT]
1924 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI ApoI AvaI AvaII
BanI BanII BbeI BbvI BcnI
BfaI BglI BmyI BsaHI BsaJI
BseRI BsiEI BsiYI BslI BsmFI
BsoBI Bsp1286I BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EagI
EclXI Eco52I EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
KasI MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfoI TaqI Tru9I TseI Tsp45I
Tsp509I TthHB8I