################################## # NO CUT ENZYMES ( 81 enzymes) # ################################## AatII Acc65I AccIII AflII Alw44I ApaLI AscI AseI AsnI Asp718I AspI AvrII BamHI BbrPI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaI BseAI BsmBI Bsp1407I BspDI BspEI BspLU11I BsrGI BssSI BstBI BstEII BstPI CelII ClaI CpoI DraIII EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HindIII HpaI KpnI MluI MroI NcoI NotI NruI NsiI NspV PacI PaeR7I PflFI PmaCI PmeI PmlI PshAI PshBI PstI PvuI RsrII SalI SapI SbfI ScaI SexAI SfuI SnaBI SpeI SphI Sse8387I SspBI SwaI Tth111I XcmI XhoI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AclI 2308 AACGTT AA^CGTT AgeI 738 ACCGGT A^CCGGT AhdI 347 GACATCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 601 CCTCAGG CC^T[ACGT]AGG Aor51HI 685 AGCGCT AGC^GCT ApaI 1103 GGGCCC GGGCC^C Asp700I 1448 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 347 GACATCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 582 GAGCTC G[AT]GC[AT]^C AviII 3051 TGCGCA TGC^GCA BbsI 2255 GTCTTC GAAGAC(2/6) BbvCI 319 GCTGAGG CCTCAGC(-5/-2) BpuAI 2255 GTCTTC GAAGAC(2/6) BsaAI 411 CACGTA [CT]AC^GT[GA] BsaBI 1385 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiHKAI 582 GAGCTC G[AT]GC[AT]^C BsiWI 1930 CGTACG C^GTACG BsmI 2703 GCATTC GAATGC(1/-1) BspMI 2035 ACCTGC ACCTGC(4/8) BsrDI 2456 GCAATG GCAATG(2/0) BssHII 1680 GCGCGC G^CGCGC BstAPI 1112 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 22 CCAAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 601 CCTCAGG CC^T[ACGT]AGG Eam1105I 347 GACATCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 402 GAAGAG CTCTTC(1/4) Eco47III 685 AGCGCT AGC^GCT Eco81I 601 CCTCAGG CC^T[ACGT]AGG EcoT14I 53 CCAAGG C^C[AT][AT]GG FspI 3051 TGCGCA TGC^GCA Ksp632I 402 GAAGAG CTCTTC(1/4) KspI 564 CCGCGG CCGC^GG MamI 1385 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 2887 CAATTG C^AATTG MunI 2887 CAATTG C^AATTG NdeI 3122 CATATG CA^TATG NheI 1961 GCTAGC G^CTAGC NspI 421 GCATGT [GA]CATG^[CT] PflMI 2796 CCAAAGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 738 ACCGGT A^CCGGT PpuMI 2004 GGGACCT [GA]G^G[AT]CC[CT] Psp1406I 2308 AACGTT AA^CGTT PvuII 1113 CAGCTG CAG^CTG SacI 582 GAGCTC GAGCT^C SacII 564 CCGCGG CCGC^GG SfcI 1832 CTACAG C^T[GA][CT]AG SfiI 1655 GGCCAAGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SgrAI 1256 CGCCGGCG C[GA]^CCGG[CT]G SmaI 1556 CCCGGG CCC^GGG SmlI 2639 CTTGAG C^T[CT][GA]AG SspI 267 AATATT AAT^ATT StuI 598 AGGCCT AGG^CCT StyI 53 CCAAGG C^C[AT][AT]GG Van91I 2796 CCAAAGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 2809 TCTAGA T^CTAGA XmaCI 1556 CCCGGG C^CCGGG XmaI 1556 CCCGGG C^CCGGG XmnI 1448 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AccI 108 GTATAC GT^[AC][GT]AC 2826 GTATAC AflIII 410 ACACGT A^C[GA][CT]GT 1600 ACGTGT AlwNI 1110 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG 1113 CAGCTGCTG BalI 1387 TGGCCA TGG^CCA 2736 TGGCCA BpmI 326 CTGGAG CTGGAG(16/14) 379 CTGGAG BsaWI 208 ACCGGA [AT]^CCGG[AT] 738 ACCGGT BsgI 1758 CTGCAC GTGCAG(16/14) 2594 GTGCAG BsmAI 405 GAGAC GTCTC(1/5) 2406 GTCTC BspHI 1381 TCATGA T^CATGA 1444 TCATGA Bst1107I 108 GTATAC GTA^TAC 2826 GTATAC BstYI 41 AGATCC [GA]^GATC[CT] 1924 AGATCC BstZ17I 108 GTATAC GTA^TAC 2826 GTATAC DrdI 1905 GACCGGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2204 GACGGGACTGTC MflI 41 AGATCC [GA]^GATC[CT] 1924 AGATCC MluNI 1387 TGGCCA TGG^CCA 2736 TGGCCA MscI 1387 TGGCCA TGG^CCA 2736 TGGCCA NaeI 1257 GCCGGC GCC^GGC 1719 GCCGGC NgoMIV 1257 GCCGGC G^CCGGC 1719 GCCGGC RcaI 1381 TCATGA T^CATGA 1444 TCATGA TfiI 607 GAATC G^A[AT]TC 2892 GAATC TspRI 691 CAGTG CA[GC]TG[ACGT][ACGT]^ 2876 CACTG XhoII 41 AGATCC [GA]^GATC[CT] 1924 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 97 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI ApoI AvaI AvaII BanI BanII BbeI BbvI BcnI BfaI BglI BmyI BsaHI BsaJI BseRI BsiEI BsiYI BslI BsmFI BsoBI Bsp1286I BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EagI EclXI Eco52I EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI KasI MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfoI TaqI Tru9I TseI Tsp45I Tsp509I TthHB8I