##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII Alw44I Aor51HI ApaI ApaLI
AscI Asp718I AviII BamHI BbeI
BbrPI BbsI BcgI BciVI BfrI
BlpI Bpu1102I BpuAI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BspLU11I
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I CelII ClaI CpoI
DraIII DrdI EagI EclXI Eco47III
Eco52I EcoO65I FseI FspI HaeII
KasI KpnI MluI MroI NaeI
NarI NgoMIV NheI NotI NruI
NspI NspV PacI PaeR7I PmaCI
PmeI PmlI Psp1406I PvuI RsrII
SacI SapI SbfI SexAI SfiI
SfoI SfuI SmlI SnaBI SpeI
SphI Sse8387I XhoI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AcyI 37 GGCGTC G[GA]^CG[CT]C
AgeI 276 ACCGGT A^CCGGT
AhdI 249 GACCCGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1942 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 507 CCTCAGG CC^T[ACGT]AGG
AspEI 249 GACCCGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 189 GTGCTC G[AT]GC[AT]^C
AvrII 2319 CCTAGG C^CTAGG
BanII 2397 GGGCTC G[GA]GC[CT]^C
BclI 2426 TGATCA T^GATCA
BglI 1818 GCCACTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 589 AGATCT A^GATCT
BlnI 2319 CCTAGG C^CTAGG
BsaAI 339 TACGTG [CT]AC^GT[GA]
BsaHI 37 GGCGTC G[GA]^CG[CT]C
BsaWI 276 ACCGGT [AT]^CCGG[AT]
BsiHKAI 189 GTGCTC G[AT]GC[AT]^C
BspHI 3655 TCATGA T^CATGA
BsrFI 276 ACCGGT [GA]^CCGG[CT]
BstAPI 3165 GCATCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 507 CCTCAGG CC^T[ACGT]AGG
Cfr10I 276 ACCGGT [GA]^CCGG[CT]
Eam1105I 249 GACCCGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 507 CCTCAGG CC^T[ACGT]AGG
EcoRV 2151 GATATC GAT^ATC
EcoT22I 4132 ATGCAT ATGCA^T
FbaI 2426 TGATCA T^GATCA
Hin1I 37 GGCGTC G[GA]^CG[CT]C
KspI 181 CCGCGG CCGC^GG
NcoI 707 CCATGG C^CATGG
NsiI 4132 ATGCAT ATGCA^T
PinAI 276 ACCGGT A^CCGGT
RcaI 3655 TCATGA T^CATGA
SacII 181 CCGCGG CCGC^GG
SalI 2259 GTCGAC G^TCGAC
SgrAI 275 CACCGGTG C[GA]^CCGG[CT]G
SmaI 319 CCCGGG CCC^GGG
StuI 2322 AGGCCT AGG^CCT
SwaI 2956 ATTTAAAT ATTT^AAAT
XcmI 1423 CCACAAAACCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 319 CCCGGG C^CCGGG
XmaI 319 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AccI 40 GTCTAC GT^[AC][GT]AC
2259 GTCGAC
AccII 22 CGCG CG^CG
182 CGCG
Asp700I 1899 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3621 GAAATGCTTC
AspI 1704 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2484 GACACTGTC
BalI 332 TGGCCA TGG^CCA
634 TGGCCA
BbvCI 825 GCTGAGG CCTCAGC(-5/-2)
2030 GCTGAGG
BpmI 1081 CTGGAG CTGGAG(16/14)
1122 CTGGAG
BsaBI 539 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2853 GATCTTCATC
BsaI 1419 GAGACC GGTCTC(1/5)
838 GGTCTC
BsgI 369 CTGCAC GTGCAG(16/14)
3511 CTGCAC
BspMI 76 ACCTGC ACCTGC(4/8)
214 ACCTGC
BsrBI 356 CCGCTC CCGCTC(-3/-3)
437 GAGCGG
BstUI 22 CGCG CG^CG
182 CGCG
BstXI 2455 CCACAGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2650 CCATCACACTGG
Cac8I 2108 GCCTGC GC[ACGT]^[ACGT]GC
3452 GCCTGC
DsaI 181 CCGCGG C^C[GA][CT]GG
707 CCATGG
EcoRI 1717 GAATTC G^AATTC
3774 GAATTC
EcoT14I 707 CCATGG C^C[AT][AT]GG
2319 CCTAGG
HgaI 1950 GACGC GACGC(5/10)
38 GCGTC
HindIII 2639 AAGCTT A^AGCTT
3049 AAGCTT
HpaI 1290 GTTAAC GTT^AAC
1344 GTTAAC
MaeII 340 ACGT A^CGT
727 ACGT
MamI 539 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2853 GATCTTCATC
MfeI 896 CAATTG C^AATTG
964 CAATTG
MluNI 332 TGGCCA TGG^CCA
634 TGGCCA
MscI 332 TGGCCA TGG^CCA
634 TGGCCA
MunI 896 CAATTG C^AATTG
964 CAATTG
MvnI 22 CGCG CG^CG
182 CGCG
NdeI 2766 CATATG CA^TATG
3309 CATATG
PflFI 1704 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2484 GACACTGTC
PflMI 977 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1908 CCAGGCTATGG
PshAI 471 GACTCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1851 GACAACAGTC
ScaI 2210 AGTACT AGT^ACT
3006 AGTACT
StyI 707 CCATGG C^C[AT][AT]GG
2319 CCTAGG
Tth111I 1704 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2484 GACACTGTC
Van91I 977 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1908 CCAGGCTATGG
XbaI 2147 TCTAGA T^CTAGA
3781 TCTAGA
XmnI 1899 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3621 GAAATGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AseI AsnI AvaI AvaII
BanI BbvI BcnI BfaI BmyI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BsrDI BsrGI BsrI BssKI BstF5I
BstNI BstYI CfoI Cfr13I DdeI
DpnII DraI DraII EaeI EarI
EcoNI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PshBI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SspBI
SspI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII