##################################
# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AvaI AvrII BamHI BanI
BbeI BbrPI BbvCI BcgI BcnI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsiEI BsiWI BsmBI BsoBI
BspDI BspEI BsrBI BsrFI BssHII
BssSI Bst1107I BstEII BstPI BstUI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoT22I FseI HaeII
HapII HgaI Hin1I HpaII KasI
KpnI KspI MamI MluI MroI
MspI MvnI NaeI NarI NciI
NgoMIV NotI NruI NsiI PaeR7I
PflMI PinAI PmaCI PmlI PshAI
Psp1406I PvuI RsrII SacII SalI
SbfI SexAI SfiI SfoI SgrAI
SmaI SnaBI Sse8387I Van91I XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AciI 1238 GCGG CCGC(-3/-1)
AlwNI 99 CAGCTACTG CAG[ACGT][ACGT][ACGT]^CTG
AspI 840 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 948 TGCGCA TGC^GCA
BalI 2445 TGGCCA TGG^CCA
BanII 1481 GAGCTC G[GA]GC[CT]^C
BclI 4128 TGATCA T^GATCA
BglI 3636 GCCTTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsgI 582 CTGCAC GTGCAG(16/14)
BsiYI 3255 CCTTAAAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 3255 CCTTAAAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 2235 GCATTC GAATGC(1/-1)
BssKI 4210 CCAGG ^CC[ACGT]GG
BstAPI 4688 GCATCATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 4177 TTCGAA TT^CGAA
BstNI 4210 CCAGG CC^[AT]GG
BstXI 3474 CCATTTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EaeI 2445 TGGCCA [CT]^GGCC[GA]
EcoRII 4210 CCAGG ^CC[AT]GG
EcoRV 2113 GATATC GAT^ATC
FauI 1238 GCGGG CCCGC(4/6)
FbaI 4128 TGATCA T^GATCA
FspI 948 TGCGCA TGC^GCA
MfeI 2543 CAATTG C^AATTG
MluNI 2445 TGGCCA TGG^CCA
MscI 2445 TGGCCA TGG^CCA
MspA1I 4448 CAGCTG C[AC]G^C[GT]G
MunI 2543 CAATTG C^AATTG
MvaI 4210 CCAGG CC^[AT]GG
NheI 1138 GCTAGC G^CTAGC
NspV 4177 TTCGAA TT^CGAA
PacI 1522 TTAATTAA TTAAT^TAA
PflFI 840 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 1762 GAGTC GAGTC(4/5)
PmeI 340 GTTTAAAC GTTT^AAAC
PvuII 4448 CAGCTG CAG^CTG
SacI 1481 GAGCTC GAGCT^C
SapI 159 GAAGAGC GCTCTTC(1/4)
ScrFI 4210 CCAGG CC^[ACGT]GG
SfuI 4177 TTCGAA TT^CGAA
SpeI 2125 ACTAGT A^CTAGT
SphI 410 GCATGC GCATG^C
StuI 1267 AGGCCT AGG^CCT
SwaI 4567 ATTTAAAT ATTT^AAAT
Tth111I 840 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AflII 1931 CTTAAG C^TTAAG
2075 CTTAAG
AlwI 394 GATCC GGATC(4/5)
4812 GATCC
Asp700I 1316 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3396 GAAGTATTTC
BciVI 2757 GGATAC GTATCC(6/5)
4555 GGATAC
BfrI 1931 CTTAAG C^TTAAG
2075 CTTAAG
BpmI 810 CTCCAG CTGGAG(16/14)
701 CTGGAG
BsmAI 1199 GTCTC GTCTC(1/5)
4717 GTCTC
BsmFI 4647 GGGAC GGGAC(10/14)
3716 GTCCC
BspMI 2160 ACCTGC ACCTGC(4/8)
1906 GCAGGT
BsrDI 1733 CATTGC GCAATG(2/0)
2006 CATTGC
BstYI 393 AGATCC [GA]^GATC[CT]
4811 AGATCC
CfoI 949 GCGC GCG^C
1745 GCGC
DraII 4476 AGGACCC [GA]G^G[ACGT]CC[CT]
4647 GGGACCT
DsaI 567 CCATGG C^C[GA][CT]GG
1581 CCATGG
EcoO109I 4476 AGGACCC [GA]G^G[ACGT]CC[CT]
4647 GGGACCT
HhaI 949 GCGC GCG^C
1745 GCGC
HinP1I 949 GCGC G^CGC
1745 GCGC
HindIII 2868 AAGCTT A^AGCTT
3316 AAGCTT
HpaI 2384 GTTAAC GTT^AAC
2806 GTTAAC
MaeII 4022 ACGT A^CGT
4486 ACGT
MflI 393 AGATCC [GA]^GATC[CT]
4811 AGATCC
NcoI 567 CCATGG C^CATGG
1581 CCATGG
NdeI 1077 CATATG CA^TATG
3792 CATATG
PpuMI 4476 AGGACCC [GA]G^G[AT]CC[CT]
4647 GGGACCT
PstI 1083 CTGCAG CTGCA^G
1904 CTGCAG
ScaI 2592 AGTACT AGT^ACT
3668 AGTACT
XcmI 1143 CCAAACTGCTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4482 CCAAACGTCATTTGG
XhoII 393 AGATCC [GA]^GATC[CT]
4811 AGATCC
XmnI 1316 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3396 GAAGTATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AccI AcsI AfaI AflIII AluI
ApoI AseI AsnI AspHI AvaII
BbsI BbvI BfaI BmyI BpuAI
BsaJI BsiHKAI Bsp1286I Bsp1407I BspHI
BspLU11I BsrGI BsrI BstF5I Cac8I
Cfr13I DdeI DpnII DraI EarI
EcoT14I Fnu4HI FokI HaeIII HincII
HindII HinfI HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MnlI
MseI MslI MwoI NdeII NlaIII
NlaIV NspI PshBI RcaI RsaI
Sau3AI Sau96I SfaNI SfcI SmlI
SspBI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I