################################## # NO CUT ENZYMES ( 113 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AvaI AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BcnI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsoBI BspDI BspEI BsrBI BsrFI BssHII BssSI Bst1107I BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoT22I FseI HaeII HapII HgaI Hin1I HpaII KasI KpnI KspI MamI MluI MroI MspI MvnI NaeI NarI NciI NgoMIV NotI NruI NsiI PaeR7I PflMI PinAI PmaCI PmlI PshAI Psp1406I PvuI RsrII SacII SalI SbfI SexAI SfiI SfoI SgrAI SmaI SnaBI Sse8387I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AciI 1238 GCGG CCGC(-3/-1) AlwNI 99 CAGCTACTG CAG[ACGT][ACGT][ACGT]^CTG AspI 840 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 948 TGCGCA TGC^GCA BalI 2445 TGGCCA TGG^CCA BanII 1481 GAGCTC G[GA]GC[CT]^C BclI 4128 TGATCA T^GATCA BglI 3636 GCCTTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsgI 582 CTGCAC GTGCAG(16/14) BsiYI 3255 CCTTAAAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 3255 CCTTAAAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmI 2235 GCATTC GAATGC(1/-1) BssKI 4210 CCAGG ^CC[ACGT]GG BstAPI 4688 GCATCATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 4177 TTCGAA TT^CGAA BstNI 4210 CCAGG CC^[AT]GG BstXI 3474 CCATTTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG EaeI 2445 TGGCCA [CT]^GGCC[GA] EcoRII 4210 CCAGG ^CC[AT]GG EcoRV 2113 GATATC GAT^ATC FauI 1238 GCGGG CCCGC(4/6) FbaI 4128 TGATCA T^GATCA FspI 948 TGCGCA TGC^GCA MfeI 2543 CAATTG C^AATTG MluNI 2445 TGGCCA TGG^CCA MscI 2445 TGGCCA TGG^CCA MspA1I 4448 CAGCTG C[AC]G^C[GT]G MunI 2543 CAATTG C^AATTG MvaI 4210 CCAGG CC^[AT]GG NheI 1138 GCTAGC G^CTAGC NspV 4177 TTCGAA TT^CGAA PacI 1522 TTAATTAA TTAAT^TAA PflFI 840 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 1762 GAGTC GAGTC(4/5) PmeI 340 GTTTAAAC GTTT^AAAC PvuII 4448 CAGCTG CAG^CTG SacI 1481 GAGCTC GAGCT^C SapI 159 GAAGAGC GCTCTTC(1/4) ScrFI 4210 CCAGG CC^[ACGT]GG SfuI 4177 TTCGAA TT^CGAA SpeI 2125 ACTAGT A^CTAGT SphI 410 GCATGC GCATG^C StuI 1267 AGGCCT AGG^CCT SwaI 4567 ATTTAAAT ATTT^AAAT Tth111I 840 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AflII 1931 CTTAAG C^TTAAG 2075 CTTAAG AlwI 394 GATCC GGATC(4/5) 4812 GATCC Asp700I 1316 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3396 GAAGTATTTC BciVI 2757 GGATAC GTATCC(6/5) 4555 GGATAC BfrI 1931 CTTAAG C^TTAAG 2075 CTTAAG BpmI 810 CTCCAG CTGGAG(16/14) 701 CTGGAG BsmAI 1199 GTCTC GTCTC(1/5) 4717 GTCTC BsmFI 4647 GGGAC GGGAC(10/14) 3716 GTCCC BspMI 2160 ACCTGC ACCTGC(4/8) 1906 GCAGGT BsrDI 1733 CATTGC GCAATG(2/0) 2006 CATTGC BstYI 393 AGATCC [GA]^GATC[CT] 4811 AGATCC CfoI 949 GCGC GCG^C 1745 GCGC DraII 4476 AGGACCC [GA]G^G[ACGT]CC[CT] 4647 GGGACCT DsaI 567 CCATGG C^C[GA][CT]GG 1581 CCATGG EcoO109I 4476 AGGACCC [GA]G^G[ACGT]CC[CT] 4647 GGGACCT HhaI 949 GCGC GCG^C 1745 GCGC HinP1I 949 GCGC G^CGC 1745 GCGC HindIII 2868 AAGCTT A^AGCTT 3316 AAGCTT HpaI 2384 GTTAAC GTT^AAC 2806 GTTAAC MaeII 4022 ACGT A^CGT 4486 ACGT MflI 393 AGATCC [GA]^GATC[CT] 4811 AGATCC NcoI 567 CCATGG C^CATGG 1581 CCATGG NdeI 1077 CATATG CA^TATG 3792 CATATG PpuMI 4476 AGGACCC [GA]G^G[AT]CC[CT] 4647 GGGACCT PstI 1083 CTGCAG CTGCA^G 1904 CTGCAG ScaI 2592 AGTACT AGT^ACT 3668 AGTACT XcmI 1143 CCAAACTGCTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 4482 CCAAACGTCATTTGG XhoII 393 AGATCC [GA]^GATC[CT] 4811 AGATCC XmnI 1316 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3396 GAAGTATTTC ######################################### # 3 OR MORE CUT ENZYMES ( 71 enzymes) # ######################################### AccI AcsI AfaI AflIII AluI ApoI AseI AsnI AspHI AvaII BbsI BbvI BfaI BmyI BpuAI BsaJI BsiHKAI Bsp1286I Bsp1407I BspHI BspLU11I BsrGI BsrI BstF5I Cac8I Cfr13I DdeI DpnII DraI EarI EcoT14I Fnu4HI FokI HaeIII HincII HindII HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MslI MwoI NdeII NlaIII NlaIV NspI PshBI RcaI RsaI Sau3AI Sau96I SfaNI SfcI SmlI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I