##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII AclI AflII AgeI AhdI
Alw44I ApaLI AscI AseI AsnI
AspEI AspI AviII BamHI BbeI
BbrPI BfrI BsaAI BsiWI BssHII
BstBI CpoI Eam1105I EcoRV FseI
FspI HincII HindII HpaI KasI
MfeI MluI MunI NarI NotI
NruI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SalI SapI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SphI SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
Acc65I 4446 GGTACC G^GTACC
AccIII 1574 TCCGGA T^CCGGA
AcyI 186 GACGCC G[GA]^CG[CT]C
Aor51HI 1212 AGCGCT AGC^GCT
Asp718I 4446 GGTACC G^GTACC
BalI 3636 TGGCCA TGG^CCA
BbvCI 2490 GCTGAGG CCTCAGC(-5/-2)
BciVI 2851 GGATAC GTATCC(6/5)
BglI 225 GCCACGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 4318 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 4318 GCTGAGC GC^T[ACGT]AGC
BsaBI 710 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 186 GACGCC G[GA]^CG[CT]C
BsaI 888 GGTCTC GGTCTC(1/5)
BsaWI 1574 TCCGGA [AT]^CCGG[AT]
BseAI 1574 TCCGGA T^CCGGA
BsmBI 3795 GAGACG CGTCTC(1/5)
BsmI 1742 GAATGC GAATGC(1/-1)
BspDI 1355 ATCGAT AT^CGAT
BspEI 1574 TCCGGA T^CCGGA
BspMI 850 ACCTGC ACCTGC(4/8)
BssSI 933 CTCGTG CACGAG(-5/-1)
Bst1107I 2534 GTATAC GTA^TAC
BstAPI 239 GCACGCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 4659 GGTCACC G^GT[ACGT]ACC
BstPI 4659 GGTCACC G^GT[ACGT]ACC
BstZ17I 2534 GTATAC GTA^TAC
CelII 4318 GCTGAGC GC^T[ACGT]AGC
ClaI 1355 ATCGAT AT^CGAT
DraI 1923 TTTAAA TTT^AAA
DrdI 1275 GACCAGGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 26 CGGCCG C^GGCCG
EclXI 26 CGGCCG C^GGCCG
Eco47III 1212 AGCGCT AGC^GCT
Eco52I 26 CGGCCG C^GGCCG
EcoNI 1668 CCTGTCTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 4659 GGTCACC G^GT[ACGT]ACC
Hin1I 186 GACGCC G[GA]^CG[CT]C
KpnI 4446 GGTACC GGTAC^C
KspI 1680 CCGCGG CCGC^GG
MamI 710 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 3636 TGGCCA TGG^CCA
MroI 1574 TCCGGA T^CCGGA
MscI 3636 TGGCCA TGG^CCA
NcoI 3176 CCATGG C^CATGG
NheI 2556 GCTAGC G^CTAGC
PshAI 150 GACAGCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1195 CTGCAG CTGCA^G
SacI 3456 GAGCTC GAGCT^C
SacII 1680 CCGCGG CCGC^GG
SbfI 1194 CCTGCAGG CCTGCA^GG
ScaI 511 AGTACT AGT^ACT
SmaI 66 CCCGGG CCC^GGG
SpeI 2224 ACTAGT A^CTAGT
Sse8387I 1194 CCTGCAGG CCTGCA^GG
StuI 1363 AGGCCT AGG^CCT
XmaCI 66 CCCGGG C^CCGGG
XmaI 66 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 2207 GTCTAC GT^[AC][GT]AC
2534 GTATAC
ApaI 525 GGGCCC GGGCC^C
819 GGGCCC
Asp700I 1578 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3476 GAAGGAATTC
AvrII 2019 CCTAGG C^CTAGG
2215 CCTAGG
BanI 168 GGTGCC G^G[CT][GA]CC
4446 GGTACC
BcgI 2580 GCAACACCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4638 GCAGTCACCTCG
BclI 2277 TGATCA T^GATCA
3704 TGATCA
BglII 1801 AGATCT A^GATCT
2859 AGATCT
BlnI 2019 CCTAGG C^CTAGG
2215 CCTAGG
BsiEI 26 CGGCCG CG[GA][CT]^CG
413 CGACCG
Bsp1407I 496 TGTACA T^GTACA
4704 TGTACA
BspHI 2326 TCATGA T^CATGA
4456 TCATGA
BsrDI 3128 CATTGC GCAATG(2/0)
3861 GCAATG
BsrGI 496 TGTACA T^GTACA
4704 TGTACA
BstXI 115 CCAGAGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2419 CCAGCTACTTGG
DraIII 930 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG
980 CACATGGTG
EcoT22I 2242 ATGCAT ATGCA^T
3947 ATGCAT
FbaI 2277 TGATCA T^GATCA
3704 TGATCA
HindIII 3693 AAGCTT A^AGCTT
4402 AAGCTT
MaeII 1345 ACGT A^CGT
2886 ACGT
NaeI 24 GCCGGC GCC^GGC
209 GCCGGC
NdeI 2699 CATATG CA^TATG
4363 CATATG
NgoMIV 24 GCCGGC G^CCGGC
209 GCCGGC
NsiI 2242 ATGCAT ATGCA^T
3947 ATGCAT
PaeR7I 2454 CTCGAG C^TCGAG
4646 CTCGAG
PvuII 540 CAGCTG CAG^CTG
4075 CAGCTG
RcaI 2326 TCATGA T^CATGA
4456 TCATGA
SspBI 496 TGTACA T^GTACA
4704 TGTACA
SspI 4339 AATATT AAT^ATT
4348 AATATT
XcmI 115 CCAGAGGGATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1210 CCAGCGCTCACCTGG
XhoI 2454 CTCGAG C^TCGAG
4646 CTCGAG
XmnI 1578 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3476 GAAGGAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
AspHI AvaI AvaII BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspLU11I BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoO109I EcoRI EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII