################################## # NO CUT ENZYMES ( 59 enzymes) # ################################## AatII AclI AflII AgeI AhdI Alw44I ApaLI AscI AseI AsnI AspEI AspI AviII BamHI BbeI BbrPI BfrI BsaAI BsiWI BssHII BstBI CpoI Eam1105I EcoRV FseI FspI HincII HindII HpaI KasI MfeI MluI MunI NarI NotI NruI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SalI SapI SexAI SfiI SfoI SfuI SgrAI SnaBI SphI SwaI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### Acc65I 4446 GGTACC G^GTACC AccIII 1574 TCCGGA T^CCGGA AcyI 186 GACGCC G[GA]^CG[CT]C Aor51HI 1212 AGCGCT AGC^GCT Asp718I 4446 GGTACC G^GTACC BalI 3636 TGGCCA TGG^CCA BbvCI 2490 GCTGAGG CCTCAGC(-5/-2) BciVI 2851 GGATAC GTATCC(6/5) BglI 225 GCCACGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 4318 GCTGAGC GC^T[ACGT]AGC Bpu1102I 4318 GCTGAGC GC^T[ACGT]AGC BsaBI 710 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 186 GACGCC G[GA]^CG[CT]C BsaI 888 GGTCTC GGTCTC(1/5) BsaWI 1574 TCCGGA [AT]^CCGG[AT] BseAI 1574 TCCGGA T^CCGGA BsmBI 3795 GAGACG CGTCTC(1/5) BsmI 1742 GAATGC GAATGC(1/-1) BspDI 1355 ATCGAT AT^CGAT BspEI 1574 TCCGGA T^CCGGA BspMI 850 ACCTGC ACCTGC(4/8) BssSI 933 CTCGTG CACGAG(-5/-1) Bst1107I 2534 GTATAC GTA^TAC BstAPI 239 GCACGCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 4659 GGTCACC G^GT[ACGT]ACC BstPI 4659 GGTCACC G^GT[ACGT]ACC BstZ17I 2534 GTATAC GTA^TAC CelII 4318 GCTGAGC GC^T[ACGT]AGC ClaI 1355 ATCGAT AT^CGAT DraI 1923 TTTAAA TTT^AAA DrdI 1275 GACCAGGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 26 CGGCCG C^GGCCG EclXI 26 CGGCCG C^GGCCG Eco47III 1212 AGCGCT AGC^GCT Eco52I 26 CGGCCG C^GGCCG EcoNI 1668 CCTGTCTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 4659 GGTCACC G^GT[ACGT]ACC Hin1I 186 GACGCC G[GA]^CG[CT]C KpnI 4446 GGTACC GGTAC^C KspI 1680 CCGCGG CCGC^GG MamI 710 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 3636 TGGCCA TGG^CCA MroI 1574 TCCGGA T^CCGGA MscI 3636 TGGCCA TGG^CCA NcoI 3176 CCATGG C^CATGG NheI 2556 GCTAGC G^CTAGC PshAI 150 GACAGCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 1195 CTGCAG CTGCA^G SacI 3456 GAGCTC GAGCT^C SacII 1680 CCGCGG CCGC^GG SbfI 1194 CCTGCAGG CCTGCA^GG ScaI 511 AGTACT AGT^ACT SmaI 66 CCCGGG CCC^GGG SpeI 2224 ACTAGT A^CTAGT Sse8387I 1194 CCTGCAGG CCTGCA^GG StuI 1363 AGGCCT AGG^CCT XmaCI 66 CCCGGG C^CCGGG XmaI 66 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccI 2207 GTCTAC GT^[AC][GT]AC 2534 GTATAC ApaI 525 GGGCCC GGGCC^C 819 GGGCCC Asp700I 1578 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3476 GAAGGAATTC AvrII 2019 CCTAGG C^CTAGG 2215 CCTAGG BanI 168 GGTGCC G^G[CT][GA]CC 4446 GGTACC BcgI 2580 GCAACACCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 4638 GCAGTCACCTCG BclI 2277 TGATCA T^GATCA 3704 TGATCA BglII 1801 AGATCT A^GATCT 2859 AGATCT BlnI 2019 CCTAGG C^CTAGG 2215 CCTAGG BsiEI 26 CGGCCG CG[GA][CT]^CG 413 CGACCG Bsp1407I 496 TGTACA T^GTACA 4704 TGTACA BspHI 2326 TCATGA T^CATGA 4456 TCATGA BsrDI 3128 CATTGC GCAATG(2/0) 3861 GCAATG BsrGI 496 TGTACA T^GTACA 4704 TGTACA BstXI 115 CCAGAGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2419 CCAGCTACTTGG DraIII 930 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG 980 CACATGGTG EcoT22I 2242 ATGCAT ATGCA^T 3947 ATGCAT FbaI 2277 TGATCA T^GATCA 3704 TGATCA HindIII 3693 AAGCTT A^AGCTT 4402 AAGCTT MaeII 1345 ACGT A^CGT 2886 ACGT NaeI 24 GCCGGC GCC^GGC 209 GCCGGC NdeI 2699 CATATG CA^TATG 4363 CATATG NgoMIV 24 GCCGGC G^CCGGC 209 GCCGGC NsiI 2242 ATGCAT ATGCA^T 3947 ATGCAT PaeR7I 2454 CTCGAG C^TCGAG 4646 CTCGAG PvuII 540 CAGCTG CAG^CTG 4075 CAGCTG RcaI 2326 TCATGA T^CATGA 4456 TCATGA SspBI 496 TGTACA T^GTACA 4704 TGTACA SspI 4339 AATATT AAT^ATT 4348 AATATT XcmI 115 CCAGAGGGATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1210 CCAGCGCTCACCTGG XhoI 2454 CTCGAG C^TCGAG 4646 CTCGAG XmnI 1578 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3476 GAAGGAATTC ######################################### # 3 OR MORE CUT ENZYMES ( 109 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI AocI ApoI AspHI AvaI AvaII BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspLU11I BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII