##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AgeI AhdI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AvrII
BamHI BbeI BciVI BglII BlnI
BlpI Bpu1102I BsaBI BseAI BsiEI
BsiWI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrGI BssHII Bst1107I BstBI
BstZ17I CelII ClaI CpoI DraI
DraIII EagI Eam1105I EclXI Eco52I
EcoRI EcoRV EcoT22I FseI HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MunI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SpeI SspBI SspI SwaI TfiI
Tsp509I XbaI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AcyI 624 GACGCC G[GA]^CG[CT]C
AflII 343 CTTAAG C^TTAAG
Alw44I 2782 GTGCAC G^TGCAC
ApaLI 2782 GTGCAC G^TGCAC
AspI 2449 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 2055 CTCGGG C^[CT]CG[GA]G
AviII 2861 TGCGCA TGC^GCA
BbrPI 1016 CACGTG CAC^GTG
BbsI 3191 GAAGAC GAAGAC(2/6)
BcgI 66 GCAGCCTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 343 CTTAAG C^TTAAG
BpuAI 3191 GAAGAC GAAGAC(2/6)
BsaHI 624 GACGCC G[GA]^CG[CT]C
BsmBI 1066 CGTCTC CGTCTC(1/5)
BsmI 2385 GAATGC GAATGC(1/-1)
BsoBI 2055 CTCGGG C^[CT]CG[GA]G
BstEII 1253 GGTCACC G^GT[ACGT]ACC
BstPI 1253 GGTCACC G^GT[ACGT]ACC
EcoO65I 1253 GGTCACC G^GT[ACGT]ACC
FspI 2861 TGCGCA TGC^GCA
Hin1I 624 GACGCC G[GA]^CG[CT]C
PflFI 2449 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1016 CACGTG CAC^GTG
PmlI 1016 CACGTG CAC^GTG
PvuII 1851 CAGCTG CAG^CTG
SacI 4037 GAGCTC GAGCT^C
SapI 21 GCTCTTC GCTCTTC(1/4)
SbfI 3265 CCTGCAGG CCTGCA^GG
SnaBI 3887 TACGTA TAC^GTA
SphI 3631 GCATGC GCATG^C
Sse8387I 3265 CCTGCAGG CCTGCA^GG
Tth111I 2449 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 3234 GTCTAC GT^[AC][GT]AC
3329 GTCTAC
AflIII 1017 ACGTGT A^C[GA][CT]GT
2373 ACGTGT
AlwI 2499 GATCC GGATC(4/5)
2961 GATCC
AocI 456 CCTCAGG CC^T[ACGT]AGG
2084 CCTAAGG
Aor51HI 543 AGCGCT AGC^GCT
4047 AGCGCT
BclI 1430 TGATCA T^GATCA
2726 TGATCA
BsaAI 1016 CACGTG [CT]AC^GT[GA]
3887 TACGTA
BsaI 419 GAGACC GGTCTC(1/5)
2110 GAGACC
BsaWI 1214 ACCGGA [AT]^CCGG[AT]
3472 TCCGGT
BsrDI 2457 CATTGC GCAATG(2/0)
3742 CATTGC
BstAPI 1601 GCAAGAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2617 GCAGGTGCTGC
BstYI 2498 AGATCC [GA]^GATC[CT]
2960 AGATCC
Bsu36I 456 CCTCAGG CC^T[ACGT]AGG
2084 CCTAAGG
DrdI 624 GACGCCTGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2476 GACTGCACAGTC
Eco47III 543 AGCGCT AGC^GCT
4047 AGCGCT
Eco81I 456 CCTCAGG CC^T[ACGT]AGG
2084 CCTAAGG
FbaI 1430 TGATCA T^GATCA
2726 TGATCA
MflI 2498 AGATCC [GA]^GATC[CT]
2960 AGATCC
NaeI 984 GCCGGC GCC^GGC
1030 GCCGGC
NgoMIV 984 GCCGGC G^CCGGC
1030 GCCGGC
StuI 1260 AGGCCT AGG^CCT
2826 AGGCCT
TaqI 2870 TCGA T^CGA
3760 TCGA
TthHB8I 2870 TCGA T^CGA
3760 TCGA
XhoII 2498 AGATCC [GA]^GATC[CT]
2960 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
ApaI AspHI AvaII BalI BanI
BanII BbvCI BbvI BcnI BfaI
BglI BmyI BpmI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BssSI BstF5I BstNI
BstUI BstXI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
Tru9I TseI Tsp45I TspRI Van91I
XcmI