##################################
# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AvaI AviII BalI BamHI
BanI BbeI BbrPI BbvCI BcgI
BclI BglI BglII BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI BsoBI BspEI BsrBI BsrFI
BssHII BssSI BstAPI BstEII BstPI
BstUI Bsu36I Cfr10I CpoI DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRV
FauI FbaI FseI FspI HaeII
HgaI Hin1I KasI KpnI KspI
MfeI MluI MluNI MroI MscI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
PacI PaeR7I PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PvuI
RsrII SacII SapI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SpeI SphI Sse8387I StuI
SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AlwNI 1490 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 1411 ATTAAT AT^TAAT
AsnI 1411 ATTAAT AT^TAAT
AspI 1681 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcnI 697 CCGGG CC^[GC]GG
BlpI 963 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 963 GCTTAGC GC^T[ACGT]AGC
BsaAI 2338 CACGTA [CT]AC^GT[GA]
BsaBI 1620 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I 706 TGTACA T^GTACA
BspDI 1627 ATCGAT AT^CGAT
BsrGI 706 TGTACA T^GTACA
Bst1107I 2862 GTATAC GTA^TAC
BstBI 1511 TTCGAA TT^CGAA
BstZ17I 2862 GTATAC GTA^TAC
CelII 963 GCTTAGC GC^T[ACGT]AGC
CfoI 1988 GCGC GCG^C
ClaI 1627 ATCGAT AT^CGAT
DraIII 8 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 447 CCTCCTCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 879 ATGCAT ATGCA^T
HapII 697 CCGG C^CGG
HhaI 1988 GCGC GCG^C
HinP1I 1988 GCGC G^CGC
HindIII 393 AAGCTT A^AGCTT
HpaI 900 GTTAAC GTT^AAC
HpaII 697 CCGG C^CGG
MamI 1620 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 549 CAGCTG C[AC]G^C[GT]G
MspI 697 CCGG C^CGG
NciI 697 CCGGG CC^[GC]GG
NsiI 879 ATGCAT ATGCA^T
NspV 1511 TTCGAA TT^CGAA
PflFI 1681 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1229 CCATCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 1411 ATTAAT AT^TAAT
PvuII 549 CAGCTG CAG^CTG
SacI 300 GAGCTC GAGCT^C
SalI 1687 GTCGAC G^TCGAC
SfuI 1511 TTCGAA TT^CGAA
SspBI 706 TGTACA T^GTACA
Tth111I 1681 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1229 CCATCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 174 TCTAGA T^CTAGA
XcmI 3362 CCAAATTACAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccI 1687 GTCGAC GT^[AC][GT]AC
2862 GTATAC
AciI 961 CCGC CCGC(-3/-1)
1696 CCGC
AvaII 3259 GGTCC G^G[AT]CC
3823 GGACC
AvrII 427 CCTAGG C^CTAGG
3495 CCTAGG
BlnI 427 CCTAGG C^CTAGG
3495 CCTAGG
BpmI 1663 CTCCAG CTGGAG(16/14)
3337 CTGGAG
BsmAI 3306 GTCTC GTCTC(1/5)
3322 GTCTC
BsmI 1800 GAATGC GAATGC(1/-1)
692 GCATTC
BspHI 1140 TCATGA T^CATGA
1354 TCATGA
BstXI 974 CCATTTAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3099 CCAAAATTATGG
BstYI 611 GGATCT [GA]^GATC[CT]
3122 AGATCC
DraII 2402 AGGCCCC [GA]G^G[ACGT]CC[CT]
3450 AGGCCCT
EcoO109I 2402 AGGCCCC [GA]G^G[ACGT]CC[CT]
3450 AGGCCCT
MaeII 2339 ACGT A^CGT
2572 ACGT
MflI 611 GGATCT [GA]^GATC[CT]
3122 AGATCC
RcaI 1140 TCATGA T^CATGA
1354 TCATGA
XhoII 611 GGATCT [GA]^GATC[CT]
3122 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AcsI AfaI AflII AflIII AluI
AlwI ApoI Asp700I AspHI BanII
BbsI BbvI BciVI BfaI BfrI
BmyI BpuAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BspLU11I
BspMI BsrDI BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraI EarI EcoRI EcoRII EcoT14I
Fnu4HI FokI HaeIII HincII HindII
HinfI HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MnlI MseI
MslI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XmnI