##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AccIII AclI AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI AspEI AvrII BamHI BbeI
BbrPI BciVI BglI BglII BlnI
BsaAI BsaBI BseAI BsiEI BsiWI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrGI BssHII Bst1107I BstBI BstZ17I
Bsu36I ClaI DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoRV
FseI HpaI KasI MamI MfeI
MroI MunI NaeI NarI NgoMIV
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI SalI SapI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SspBI SspI
SwaI XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AatII 631 GACGTC GACGT^C
Acc65I 3220 GGTACC G^GTACC
AflII 1328 CTTAAG C^TTAAG
AflIII 155 ACGCGT A^C[GA][CT]GT
ApaI 3029 GGGCCC GGGCC^C
Asp700I 2237 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3220 GGTACC G^GTACC
AspI 2841 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 2997 CGAGTGAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 710 TGATCA T^GATCA
BfrI 1328 CTTAAG C^TTAAG
BsaWI 500 ACCGGA [AT]^CCGG[AT]
BsmI 2496 GCATTC GAATGC(1/-1)
BspHI 1965 TCATGA T^CATGA
BsrDI 434 GCAATG GCAATG(2/0)
BsrFI 89 ACCGGC [GA]^CCGG[CT]
Cfr10I 89 ACCGGC [GA]^CCGG[CT]
CpoI 3040 CGGACCG CG^G[AT]CCG
DraI 3343 TTTAAA TTT^AAA
EcoRI 2296 GAATTC G^AATTC
EcoT22I 3453 ATGCAT ATGCA^T
FbaI 710 TGATCA T^GATCA
HindIII 520 AAGCTT A^AGCTT
KpnI 3220 GGTACC GGTAC^C
KspI 130 CCGCGG CCGC^GG
MluI 155 ACGCGT A^CGCGT
NheI 1941 GCTAGC G^CTAGC
NsiI 3453 ATGCAT ATGCA^T
PflFI 2841 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 1965 TCATGA T^CATGA
RsrII 3040 CGGACCG CG^G[AT]CCG
SacII 130 CCGCGG CCGC^GG
SbfI 3023 CCTGCAGG CCTGCA^GG
Sse8387I 3023 CCTGCAGG CCTGCA^GG
Tth111I 2841 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1037 CCAGCCAAAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2237 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 613 GTCTAC GT^[AC][GT]AC
3379 GTAGAC
AcsI 2296 GAATTC [GA]^AATT[CT]
2325 AAATTC
ApoI 2296 GAATTC [GA]^AATT[CT]
2325 AAATTC
AspHI 907 GAGCTC G[AT]GC[AT]^C
1922 GAGCTC
AviII 482 TGCGCA TGC^GCA
1973 TGCGCA
BfaI 1445 CTAG C^TAG
1942 CTAG
BsiHKAI 907 GAGCTC G[AT]GC[AT]^C
1922 GAGCTC
BstAPI 474 GCAGATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3446 GCATCAAATGC
BstXI 2102 CCAGAGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2151 CCAGGTCTGTGG
BstYI 299 GGATCT [GA]^GATC[CT]
476 AGATCC
DraIII 1849 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
2826 CACCAGGTG
EcoNI 2145 CCTTTTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3224 CCTCGCCGAGG
FspI 482 TGCGCA TGC^GCA
1973 TGCGCA
HaeII 141 GGCGCT [GA]GCGC^[CT]
1618 AGCGCC
HincII 1545 GTCAAC GT[CT]^[GA]AC
3111 GTCAAC
HindII 1545 GTCAAC GT[CT]^[GA]AC
3111 GTCAAC
MaeI 1445 CTAG C^TAG
1942 CTAG
MflI 299 GGATCT [GA]^GATC[CT]
476 AGATCC
NcoI 1642 CCATGG C^CATGG
2212 CCATGG
NdeI 2172 CATATG CA^TATG
2536 CATATG
SacI 907 GAGCTC GAGCT^C
1922 GAGCTC
SphI 485 GCATGC GCATG^C
3455 GCATGC
XhoII 299 GGATCT [GA]^GATC[CT]
476 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcnI BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BssSI BstEII BstF5I BstNI
BstPI BstUI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I