##################################
# NO CUT ENZYMES ( 102 enzymes) #
##################################
Acc65I AccII AccIII AclI AflIII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI Asp718I AspEI AviII
BbeI BbrPI BcgI BglI BglII
BlpI Bpu1102I BsaAI BsaBI BseAI
BsiEI BsiWI BsmBI BsmI BspDI
BspEI BspLU11I BsrBI BsrDI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstXI BstZ17I CelII ClaI
CpoI DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoT22I
FseI FspI HaeII HgaI HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MspA1I MunI MvnI
NaeI NarI NcoI NgoMIV NotI
NruI NsiI NspI NspV PacI
PinAI PmaCI PmeI PmlI Psp1406I
PvuI PvuII RsrII SacII SalI
SapI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SphI SwaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 3070 GACGTC GACGT^C
AccI 2726 GTCTAC GT^[AC][GT]AC
AcyI 3070 GACGTC G[GA]^CG[CT]C
AflII 2160 CTTAAG C^TTAAG
AspI 2039 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3405 CCTAGG C^CTAGG
BamHI 2528 GGATCC G^GATCC
BanI 2088 GGCACC G^G[CT][GA]CC
BciVI 886 GGATAC GTATCC(6/5)
BclI 1629 TGATCA T^GATCA
BfrI 2160 CTTAAG C^TTAAG
BlnI 3405 CCTAGG C^CTAGG
BsaHI 3070 GACGTC G[GA]^CG[CT]C
BsaWI 3586 ACCGGA [AT]^CCGG[AT]
Bsp1407I 2838 TGTACA T^GTACA
BspHI 2885 TCATGA T^CATGA
BsrFI 2126 ACCGGC [GA]^CCGG[CT]
BsrGI 2838 TGTACA T^GTACA
BstAPI 3559 GCAGGGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI 1933 GCGC GCG^C
Cfr10I 2126 ACCGGC [GA]^CCGG[CT]
DraI 171 TTTAAA TTT^AAA
DraIII 2753 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 2347 CCTCCCATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 1894 CCCGC CCCGC(4/6)
FbaI 1629 TGATCA T^GATCA
HhaI 1933 GCGC GCG^C
Hin1I 3070 GACGTC G[GA]^CG[CT]C
HinP1I 1933 GCGC G^CGC
HindIII 1112 AAGCTT A^AGCTT
PaeR7I 262 CTCGAG C^TCGAG
PflFI 2039 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2616 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 2375 GACTCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2885 TCATGA T^CATGA
SacI 259 GAGCTC GAGCT^C
SbfI 2544 CCTGCAGG CCTGCA^GG
Sse8387I 2544 CCTGCAGG CCTGCA^GG
SspBI 2838 TGTACA T^GTACA
SspI 92 AATATT AAT^ATT
Tth111I 2039 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2616 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2215 TCTAGA T^CTAGA
XcmI 645 CCAGAAATCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 262 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
Asp700I 2863 GAACTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2994 GAATCATTTC
AvaI 262 CTCGAG C^[CT]CG[GA]G
912 CCCGAG
BanII 259 GAGCTC G[GA]GC[CT]^C
2939 GAGCCC
BcnI 281 CCGGG CC^[GC]GG
2627 CCCGG
BpmI 1882 CTCCAG CTGGAG(16/14)
748 CTGGAG
BsaI 1744 GAGACC GGTCTC(1/5)
3024 GGTCTC
BseRI 2927 CTCCTC GAGGAG(10/8)
2788 GAGGAG
BsgI 2953 CTGCAC GTGCAG(16/14)
3369 CTGCAC
BsoBI 262 CTCGAG C^[CT]CG[GA]G
912 CCCGAG
DraII 485 AGGTCCC [GA]G^G[ACGT]CC[CT]
1226 GGGACCT
EarI 491 CTCTTC CTCTTC(1/4)
2608 CTCTTC
EcoO109I 485 AGGTCCC [GA]G^G[ACGT]CC[CT]
1226 GGGACCT
EcoRV 579 GATATC GAT^ATC
3334 GATATC
Ksp632I 491 CTCTTC CTCTTC(1/4)
2608 CTCTTC
NciI 281 CCGGG CC^[GC]GG
2627 CCCGG
NdeI 242 CATATG CA^TATG
3361 CATATG
NheI 1570 GCTAGC G^CTAGC
2595 GCTAGC
PpuMI 485 AGGTCCC [GA]G^G[AT]CC[CT]
1226 GGGACCT
ScaI 58 AGTACT AGT^ACT
3155 AGTACT
XmnI 2863 GAACTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2994 GAATCATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AseI AsnI
AspHI AvaII BalI BbsI BbvCI
BbvI BfaI BmyI BpuAI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstYI Bsu36I Cac8I Cfr13I
DdeI DpnII EaeI Eco81I EcoRI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HincII HindII HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspI MvaI
MwoI NdeII NlaIII NlaIV PleI
PshBI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SpeI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII