##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AccIII AclI AcsI AflII AgeI
AhdI Alw44I Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I AspEI
AspI BalI BcgI BciVI BclI
BfrI BsaBI BsaWI BseAI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I ClaI DraI
DraIII DrdI Eam1105I Eco47III EcoO65I
EcoRI EcoRV FbaI HindIII HpaI
MamI MluNI MroI MscI NcoI
NdeI NheI NotI NruI NspV
PacI PflFI PinAI PmeI PshBI
Psp1406I RcaI SacI SalI SbfI
SexAI SfuI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI TfiI
Tth111I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
Acc65I 337 GGTACC G^GTACC
AccI 762 GTCTAC GT^[AC][GT]AC
Asp718I 337 GGTACC G^GTACC
AspHI 2804 GTGCTC G[AT]GC[AT]^C
AvrII 2704 CCTAGG C^CTAGG
BamHI 1291 GGATCC G^GATCC
BbrPI 3633 CACGTG CAC^GTG
BglII 360 AGATCT A^GATCT
BlnI 2704 CCTAGG C^CTAGG
BsaAI 3633 CACGTG [CT]AC^GT[GA]
BsiHKAI 2804 GTGCTC G[AT]GC[AT]^C
BsiWI 565 CGTACG C^GTACG
BspMI 1831 ACCTGC ACCTGC(4/8)
CpoI 2425 CGGACCG CG^G[AT]CCG
EcoT22I 3625 ATGCAT ATGCA^T
FseI 987 GGCCGGCC GGCCGG^CC
KpnI 337 GGTACC GGTAC^C
MfeI 3022 CAATTG C^AATTG
MluI 3271 ACGCGT A^CGCGT
MseI 796 TTAA T^TAA
MunI 3022 CAATTG C^AATTG
NsiI 3625 ATGCAT ATGCA^T
NspI 3627 GCATGC [GA]CATG^[CT]
PaeR7I 1324 CTCGAG C^TCGAG
PmaCI 3633 CACGTG CAC^GTG
PmlI 3633 CACGTG CAC^GTG
PshAI 1367 GACCGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 3300 CGATCG CGAT^CG
RsrII 2425 CGGACCG CG^G[AT]CCG
SapI 611 GCTCTTC GCTCTTC(1/4)
ScaI 1624 AGTACT AGT^ACT
SfiI 3310 GGCCCCAGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 3627 GCATGC GCATG^C
Tru9I 796 TTAA T^TAA
Tsp509I 3023 AATT ^AATT
XhoI 1324 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AatII 887 GACGTC GACGT^C
1442 GACGTC
AviII 688 TGCGCA TGC^GCA
1173 TGCGCA
BbsI 1374 GTCTTC GAAGAC(2/6)
1458 GTCTTC
BbvCI 1703 CCTCAGC CCTCAGC(-5/-2)
2473 CCTCAGC
BfaI 639 CTAG C^TAG
2705 CTAG
BpuAI 1374 GTCTTC GAAGAC(2/6)
1458 GTCTTC
BsaI 1476 GAGACC GGTCTC(1/5)
3367 GGTCTC
BsgI 1772 CTGCAC GTGCAG(16/14)
3275 GTGCAG
BsmBI 2761 GAGACG CGTCTC(1/5)
2908 GAGACG
BsrBI 2403 CCGCTC CCGCTC(-3/-3)
1917 GAGCGG
BsrI 98 ACTGG ACTGG(1/-1)
2537 ACTGG
BssSI 3291 CACGAG CACGAG(-5/-1)
3452 CACGAG
BstAPI 216 GCAGAAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1526 GCAGCGGCTGC
BstYI 360 AGATCT [GA]^GATC[CT]
1291 GGATCC
EarI 594 CTCTTC CTCTTC(1/4)
612 CTCTTC
FspI 688 TGCGCA TGC^GCA
1173 TGCGCA
HincII 258 GTCAAC GT[CT]^[GA]AC
2957 GTTGAC
HindII 258 GTCAAC GT[CT]^[GA]AC
2957 GTTGAC
Ksp632I 594 CTCTTC CTCTTC(1/4)
612 CTCTTC
MaeI 639 CTAG C^TAG
2705 CTAG
MflI 360 AGATCT [GA]^GATC[CT]
1291 GGATCC
XcmI 2452 CCACGGGCCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2826 CCACCCGCGGAGTGG
XhoII 360 AGATCT [GA]^GATC[CT]
1291 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AocI ApaI
AvaI AvaII BanI BanII BbeI
BbvI BcnI BglI BlpI BmyI
BpmI Bpu1102I BsaHI BsaJI BseRI
BsiEI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrFI BssKI BstF5I
BstNI BstUI BstXI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EagI
EclXI Eco52I Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI KspI MaeII MaeIII
MboI MboII MnlI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NdeII NgoMIV NlaIII
NlaIV PflMI PleI PpuMI PstI
PvuII RsaI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SgrAI
SmaI SmlI StyI TaqI TseI
Tsp45I TspRI TthHB8I Van91I XmaCI
XmaI