##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI Alw44I AocI ApaI ApaLI
AscI Asp718I AspEI AspHI AviII
AvrII BalI BbrPI BclI BglI
BlnI BsaBI BsaWI BseAI BseRI
BsiEI BsiHKAI BsiWI BsmBI BspDI
BspEI BsrBI BsrFI BssHII BssSI
BstAPI BstBI Bsu36I Cfr10I ClaI
CpoI EaeI EagI Eam1105I EclXI
Eco52I Eco81I FbaI FseI FspI
HincII HindII HpaI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MunI NaeI NgoMIV NheI
NotI NruI NspV PacI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SfiI
SfuI SgrAI SpeI Sse8387I SwaI
XbaI XcmI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AcyI 3637 GGCGCC G[GA]^CG[CT]C
AflII 547 CTTAAG C^TTAAG
AflIII 4192 ACATGT A^C[GA][CT]GT
AlwNI 138 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 108 AGCGCT AGC^GCT
AvaII 86 GGTCC G^G[AT]CC
BamHI 2436 GGATCC G^GATCC
BanII 2525 GAGCCC G[GA]GC[CT]^C
BbeI 3637 GGCGCC GGCGC^C
BcgI 3537 GCAGGTGGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 547 CTTAAG C^TTAAG
BglII 930 AGATCT A^GATCT
BlpI 2522 GCTGAGC GC^T[ACGT]AGC
BmyI 2525 GAGCCC G[AGT]GC[ACT]^C
Bpu1102I 2522 GCTGAGC GC^T[ACGT]AGC
BsaAI 2357 TACGTA [CT]AC^GT[GA]
BsaHI 3637 GGCGCC G[GA]^CG[CT]C
BsmFI 3647 GTCCC GGGAC(10/14)
Bsp1286I 2525 GAGCCC G[AGT]GC[ACT]^C
Bsp1407I 302 TGTACA T^GTACA
BspLU11I 4192 ACATGT A^CATGT
BsrDI 3864 GCAATG GCAATG(2/0)
BsrGI 302 TGTACA T^GTACA
Bst1107I 2172 GTATAC GTA^TAC
BstEII 2480 GGTAACC G^GT[ACGT]ACC
BstPI 2480 GGTAACC G^GT[ACGT]ACC
BstZ17I 2172 GTATAC GTA^TAC
CelII 2522 GCTGAGC GC^T[ACGT]AGC
DraII 2345 AGGCCCT [GA]G^G[ACGT]CC[CT]
DrdI 2290 GACAGAACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 108 AGCGCT AGC^GCT
EcoO109I 2345 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 2480 GGTAACC G^GT[ACGT]ACC
EcoRV 2711 GATATC GAT^ATC
Hin1I 3637 GGCGCC G[GA]^CG[CT]C
KasI 3637 GGCGCC G^GCGCC
NarI 3637 GGCGCC GG^CGCC
NdeI 918 CATATG CA^TATG
PaeR7I 2961 CTCGAG C^TCGAG
PflMI 3424 CCAAAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 4159 CTGCAG CTGCA^G
PvuII 2520 CAGCTG CAG^CTG
SexAI 2787 ACCAGGT A^CC[AT]GGT
SfoI 3637 GGCGCC GGC^GCC
SmaI 3689 CCCGGG CCC^GGG
SnaBI 2357 TACGTA TAC^GTA
SphI 2940 GCATGC GCATG^C
SspBI 302 TGTACA T^GTACA
Van91I 3424 CCAAAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 2961 CTCGAG C^TCGAG
XmaCI 3689 CCCGGG C^CCGGG
XmaI 3689 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 2172 GTATAC GT^[AC][GT]AC
2222 GTAGAC
AseI 1861 ATTAAT AT^TAAT
3178 ATTAAT
AsnI 1861 ATTAAT AT^TAAT
3178 ATTAAT
BanI 2373 GGCACC G^G[CT][GA]CC
3637 GGCGCC
BbvCI 2967 GCTGAGG CCTCAGC(-5/-2)
3665 GCTGAGG
BspHI 2281 TCATGA T^CATGA
3276 TCATGA
BspMI 2376 ACCTGC ACCTGC(4/8)
3537 GCAGGT
BstYI 930 AGATCT [GA]^GATC[CT]
2436 GGATCC
DraIII 2041 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
4000 CACACAGTG
EcoNI 610 CCTCAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2782 CCTAGACCAGG
FauI 1241 GCGGG CCCGC(4/6)
1493 GCGGG
HaeII 108 AGCGCT [GA]GCGC^[CT]
3637 GGCGCC
HgaI 2063 GACGC GACGC(5/10)
81 GCGTC
MaeII 2226 ACGT A^CGT
2358 ACGT
MflI 930 AGATCT [GA]^GATC[CT]
2436 GGATCC
MspA1I 11 CAGCGG C[AC]G^C[GT]G
2520 CAGCTG
NcoI 212 CCATGG C^CATGG
540 CCATGG
PshBI 1861 ATTAAT AT^TAAT
3178 ATTAAT
RcaI 2281 TCATGA T^CATGA
3276 TCATGA
XhoII 930 AGATCT [GA]^GATC[CT]
2436 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI Asp700I AspI AvaI
BbsI BbvI BciVI BcnI BfaI
BpmI BpuAI BsaI BsaJI BsgI
BsiYI BslI BsmAI BsmI BsoBI
BsrI BssKI BstF5I BstNI BstUI
BstXI Cac8I CfoI Cfr13I DdeI
DpnII DraI DsaI EarI EcoRI
EcoRII EcoT14I EcoT22I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MnlI
MseI MslI MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NsiI NspI PflFI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I XmnI