##################################
# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII Acc65I AccII AccIII AciI
AclI AcyI AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspI AviII
AvrII BalI BamHI BbeI BbrPI
BbvCI BcgI BlnI BsaAI BsaHI
BsaWI BseAI BseRI BsiEI BsiWI
BspDI BspEI BspLU11I BsrBI BsrFI
BssHII BssSI BstAPI BstEII BstPI
BstUI Bsu36I Cfr10I ClaI CpoI
DraIII EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoT14I FauI FseI FspI HaeII
HgaI Hin1I HincII HindII HpaI
KasI KpnI KspI MaeII MluI
MluNI MroI MscI MspA1I MvnI
NaeI NarI NcoI NgoMIV NotI
NruI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
PvuII RsrII SacII SalI SapI
SbfI SfiI SfoI SgrAI SmaI
SnaBI SpeI Sse8387I StyI Tth111I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccI 1657 GTATAC GT^[AC][GT]AC
AlwNI 2445 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 1465 GAGCTC G[AT]GC[AT]^C
BanI 1792 GGTGCC G^G[CT][GA]CC
BciVI 3101 GGATAC GTATCC(6/5)
BcnI 1514 CCCGG CC^[GC]GG
BglI 289 GCCAAGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 2737 CTGGAG CTGGAG(16/14)
BsaBI 374 GATAGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2666 GGTCTC GGTCTC(1/5)
BsiHKAI 1465 GAGCTC G[AT]GC[AT]^C
BsmBI 1873 GAGACG CGTCTC(1/5)
BsmI 3704 GAATGC GAATGC(1/-1)
BsrDI 216 GCAATG GCAATG(2/0)
Bst1107I 1657 GTATAC GTA^TAC
BstBI 818 TTCGAA TT^CGAA
BstZ17I 1657 GTATAC GTA^TAC
CfoI 1420 GCGC GCG^C
DrdI 478 GACTGGTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 898 ATGCAT ATGCA^T
HapII 1515 CCGG C^CGG
HhaI 1420 GCGC GCG^C
HinP1I 1420 GCGC G^CGC
HpaII 1515 CCGG C^CGG
MamI 374 GATAGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspI 1515 CCGG C^CGG
NciI 1514 CCCGG CC^[GC]GG
NheI 2195 GCTAGC G^CTAGC
NsiI 898 ATGCAT ATGCA^T
NspV 818 TTCGAA TT^CGAA
PacI 268 TTAATTAA TTAAT^TAA
PpuMI 1208 AGGTCCT [GA]G^G[AT]CC[CT]
SacI 1465 GAGCTC GAGCT^C
SexAI 2662 ACCTGGT A^CC[AT]GGT
SfuI 818 TTCGAA TT^CGAA
StuI 2513 AGGCCT AGG^CCT
XcmI 3026 CCACATGAGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AflII 211 CTTAAG C^TTAAG
3307 CTTAAG
AvaI 1468 CTCGGG C^[CT]CG[GA]G
2781 CCCGAG
BanII 1465 GAGCTC G[GA]GC[CT]^C
2984 GAGCCC
BbsI 1276 GAAGAC GAAGAC(2/6)
3108 GTCTTC
BclI 157 TGATCA T^GATCA
2958 TGATCA
BfrI 211 CTTAAG C^TTAAG
3307 CTTAAG
BglII 1994 AGATCT A^GATCT
3116 AGATCT
BlpI 2527 GCTGAGC GC^T[ACGT]AGC
3378 GCTGAGC
Bpu1102I 2527 GCTGAGC GC^T[ACGT]AGC
3378 GCTGAGC
BpuAI 1276 GAAGAC GAAGAC(2/6)
3108 GTCTTC
BsoBI 1468 CTCGGG C^[CT]CG[GA]G
2781 CCCGAG
Bsp1407I 54 TGTACA T^GTACA
70 TGTACA
BspMI 1458 GCAGGT ACCTGC(4/8)
2930 GCAGGT
BsrGI 54 TGTACA T^GTACA
70 TGTACA
CelII 2527 GCTGAGC GC^T[ACGT]AGC
3378 GCTGAGC
DraII 1208 AGGTCCT [GA]G^G[ACGT]CC[CT]
1833 AGGGCCT
DsaI 1289 CCACGG C^C[GA][CT]GG
1547 CCGTGG
EcoNI 1212 CCTATTTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3214 CCTAATCAAGG
EcoO109I 1208 AGGTCCT [GA]G^G[ACGT]CC[CT]
1833 AGGGCCT
EcoRV 378 GATATC GAT^ATC
2682 GATATC
FbaI 157 TGATCA T^GATCA
2958 TGATCA
MfeI 2164 CAATTG C^AATTG
3169 CAATTG
MunI 2164 CAATTG C^AATTG
3169 CAATTG
NdeI 2067 CATATG CA^TATG
2952 CATATG
PflMI 2 CCATTCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1542 CCAGTCCGTGG
PstI 126 CTGCAG CTGCA^G
3549 CTGCAG
ScaI 195 AGTACT AGT^ACT
1119 AGTACT
SphI 900 GCATGC GCATG^C
2601 GCATGC
SspBI 54 TGTACA T^GTACA
70 TGTACA
SwaI 275 ATTTAAAT ATTT^AAAT
357 ATTTAAAT
TaqI 819 TCGA T^CGA
1385 TCGA
TthHB8I 819 TCGA T^CGA
1385 TCGA
Van91I 2 CCATTCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1542 CCAGTCCGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AseI AsnI Asp700I AvaII BbvI
BfaI BmyI BsaJI BsgI BsiYI
BslI BsmAI BsmFI Bsp1286I BspHI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I Cfr13I DdeI DpnII
DraI EarI EcoRII Fnu4HI FokI
HaeIII HindIII HinfI HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PshBI RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII XmnI