##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AccIII AgeI Aor51HI AscI AseI
AsnI AviII AvrII BbeI BcgI
BciVI BclI BglI BlnI BlpI
Bpu1102I BsaBI BseAI BsgI BsiWI
Bsp1407I BspDI BspEI BspLU11I BsrDI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I CelII ClaI CpoI
DraI DraIII EagI EclXI Eco47III
Eco52I EcoO65I FbaI FseI FspI
KasI KspI MamI MfeI MluI
MroI MunI NaeI NarI NgoMIV
NheI NotI NruI NspI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI PvuI PvuII RsrII SacII
SalI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
SspBI SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 3695 GACGTC GACGT^C
Acc65I 2015 GGTACC G^GTACC
AccI 1451 GTAGAC GT^[AC][GT]AC
AclI 1074 AACGTT AA^CGTT
AcyI 3695 GACGTC G[GA]^CG[CT]C
AflII 2601 CTTAAG C^TTAAG
AflIII 9 ACGTGT A^C[GA][CT]GT
AhdI 3426 GACACCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2554 GTGCAC G^TGCAC
ApaLI 2554 GTGCAC G^TGCAC
Asp718I 2015 GGTACC G^GTACC
AspEI 3426 GACACCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 2684 TGGCCA TGG^CCA
BfrI 2601 CTTAAG C^TTAAG
BsaHI 3695 GACGTC G[GA]^CG[CT]C
BsiEI 61 CGGTCG CG[GA][CT]^CG
BsmI 159 GAATGC GAATGC(1/-1)
BsrBI 821 GAGCGG CCGCTC(-3/-3)
BsrFI 921 ACCGGC [GA]^CCGG[CT]
BssSI 2459 CACGAG CACGAG(-5/-1)
BstAPI 2063 GCAGCGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 921 ACCGGC [GA]^CCGG[CT]
DrdI 2179 GACCACCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3426 GACACCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Hin1I 3695 GACGTC G[GA]^CG[CT]C
HpaI 1932 GTTAAC GTT^AAC
KpnI 2015 GGTACC GGTAC^C
MluNI 2684 TGGCCA TGG^CCA
MscI 2684 TGGCCA TGG^CCA
MspA1I 1833 CCGCTG C[AC]G^C[GT]G
NcoI 3323 CCATGG C^CATGG
NdeI 3207 CATATG CA^TATG
Psp1406I 1074 AACGTT AA^CGTT
SbfI 963 CCTGCAGG CCTGCA^GG
ScaI 3454 AGTACT AGT^ACT
Sse8387I 963 CCTGCAGG CCTGCA^GG
StuI 1381 AGGCCT AGG^CCT
XbaI 330 TCTAGA T^CTAGA
XcmI 3021 CCATACGAGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
ApaI 266 GGGCCC GGGCC^C
3793 GGGCCC
AspI 1846 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2931 GACCTTGTC
BamHI 42 GGATCC G^GATCC
852 GGATCC
BbrPI 8 CACGTG CAC^GTG
2226 CACGTG
BcnI 86 CCGGG CC^[GC]GG
480 CCCGG
BsaWI 1736 ACCGGA [AT]^CCGG[AT]
2027 ACCGGA
BsmBI 805 GAGACG CGTCTC(1/5)
3693 GAGACG
BspMI 2420 ACCTGC ACCTGC(4/8)
2558 ACCTGC
BstXI 754 CCAAGGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3097 CCAGTCTCTTGG
EcoRI 413 GAATTC G^AATTC
3497 GAATTC
EcoRV 1258 GATATC GAT^ATC
2261 GATATC
EcoT22I 3071 ATGCAT ATGCA^T
3784 ATGCAT
HaeII 51 GGCGCT [GA]GCGC^[CT]
2075 GGCGCT
HindIII 1911 AAGCTT A^AGCTT
2247 AAGCTT
MslI 2222 CATTCACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2547 CACTGGAGTG
NciI 86 CCGGG CC^[GC]GG
480 CCCGG
NsiI 3071 ATGCAT ATGCA^T
3784 ATGCAT
PflFI 1846 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2931 GACCTTGTC
PmaCI 8 CACGTG CAC^GTG
2226 CACGTG
PmlI 8 CACGTG CAC^GTG
2226 CACGTG
SspI 1480 AATATT AAT^ATT
3046 AATATT
Tth111I 1846 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2931 GACCTTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI AocI ApoI Asp700I
AspHI AvaI AvaII BanI BanII
BbsI BbvCI BbvI BfaI BglII
BmyI BpmI BpuAI BsaAI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspHI
BsrI BssKI BstF5I BstNI BstUI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MspI
MvaI MvnI MwoI NdeII NlaIII
NlaIV PflMI PleI PpuMI PstI
RcaI RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmnI