##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AccIII AclI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp700I AspEI AspHI
AspI AviII AvrII BalI BamHI
BbrPI BbvCI BciVI BfrI BglII
BlnI BsaAI BsaBI BseAI BsiHKAI
BsiWI BsmI BspDI BspEI BspLU11I
BstBI BstEII BstPI ClaI CpoI
Eam1105I Eco47III EcoO65I EcoRV FspI
MamI MfeI MluI MluNI MroI
MscI MunI NcoI NdeI NheI
NotI NruI NspV PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SacI SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SnaBI Sse8387I StuI
SwaI TaqI Tth111I TthHB8I XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AatII 194 GACGTC GACGT^C
Acc65I 2552 GGTACC G^GTACC
AocI 727 CCTAAGG CC^T[ACGT]AGG
Asp718I 2552 GGTACC G^GTACC
BbsI 78 GAAGAC GAAGAC(2/6)
BcgI 96 GCAGATAGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 965 TGATCA T^GATCA
BlpI 91 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 91 GCTCAGC GC^T[ACGT]AGC
BpuAI 78 GAAGAC GAAGAC(2/6)
Bsp1407I 1940 TGTACA T^GTACA
BspHI 2905 TCATGA T^CATGA
BsrGI 1940 TGTACA T^GTACA
Bst1107I 3803 GTATAC GTA^TAC
BstZ17I 3803 GTATAC GTA^TAC
Bsu36I 727 CCTAAGG CC^T[ACGT]AGG
CelII 91 GCTCAGC GC^T[ACGT]AGC
DraI 2733 TTTAAA TTT^AAA
DrdI 476 GACGGGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 727 CCTAAGG CC^T[ACGT]AGG
EcoRI 4369 GAATTC G^AATTC
EcoT22I 1496 ATGCAT ATGCA^T
FbaI 965 TGATCA T^GATCA
FseI 1055 GGCCGGCC GGCCGG^CC
HindIII 3262 AAGCTT A^AGCTT
HpaI 4110 GTTAAC GTT^AAC
KpnI 2552 GGTACC GGTAC^C
NsiI 1496 ATGCAT ATGCA^T
PacI 4291 TTAATTAA TTAAT^TAA
PshAI 2450 GACAACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2905 TCATGA T^CATGA
SpeI 1511 ACTAGT A^CTAGT
SphI 2930 GCATGC GCATG^C
SspBI 1940 TGTACA T^GTACA
XcmI 2708 CCACCCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcsI 2874 AAATTT [GA]^AATT[CT]
4369 GAATTC
AlwI 2218 GATCC GGATC(4/5)
1176 GGATC
ApoI 2874 AAATTT [GA]^AATT[CT]
4369 GAATTC
BsaWI 2585 TCCGGT [AT]^CCGG[AT]
3039 ACCGGA
BssHII 817 GCGCGC G^CGCGC
1384 GCGCGC
BssSI 150 CACGAG CACGAG(-5/-1)
2759 CACGAG
BstAPI 2262 GCAAGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2373 GCAGTATCTGC
BstXI 191 CCAGACGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3643 CCAAAGAATTGG
BstYI 1176 GGATCT [GA]^GATC[CT]
2217 AGATCC
DraIII 3026 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
3126 CACAACGTG
EcoT14I 248 CCTTGG C^C[AT][AT]GG
4273 CCTTGG
MflI 1176 GGATCT [GA]^GATC[CT]
2217 AGATCC
NspI 981 ACATGC [GA]CATG^[CT]
2930 GCATGC
SmlI 1903 CTCAAG C^T[CT][GA]AG
3371 CTTGAG
StyI 248 CCTTGG C^C[AT][AT]GG
4273 CCTTGG
XbaI 3507 TCTAGA T^CTAGA
3575 TCTAGA
XhoII 1176 GGATCT [GA]^GATC[CT]
2217 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwNI ApaI AvaI AvaII
BanI BanII BbeI BbvI BcnI
BfaI BglI BmyI BpmI BsaHI
BsaI BsaJI BseRI BsgI BsiEI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EarI EclXI Eco52I EcoNI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI KasI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NdeII NgoMIV NlaIII
NlaIV PflMI PleI PpuMI PstI
PvuII RsaI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmaI
SspI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I XmaCI XmaI