################################## # NO CUT ENZYMES ( 88 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BamHI BbeI BfrI BglI BlnI BsaI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspLU11I BsrGI BssHII Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI Eam1105I Eco47III EcoO65I EcoRV EcoT22I FseI FspI HaeII HpaI KasI KpnI MfeI MluI MroI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I RsrII SalI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SspBI SwaI XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AccI 898 GTCTAC GT^[AC][GT]AC AocI 994 CCTAAGG CC^T[ACGT]AGG AspI 2256 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC BbrPI 1860 CACGTG CAC^GTG BbvCI 3234 CCTCAGC CCTCAGC(-5/-2) BciVI 3432 GTATCC GTATCC(6/5) BlpI 2653 GCTTAGC GC^T[ACGT]AGC Bpu1102I 2653 GCTTAGC GC^T[ACGT]AGC BsaBI 1908 GATTCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 298 ACCGGA [AT]^CCGG[AT] BsmBI 490 GAGACG CGTCTC(1/5) BspHI 1583 TCATGA T^CATGA BsrBI 868 GAGCGG CCGCTC(-3/-3) BssSI 29 CTCGTG CACGAG(-5/-1) Bsu36I 994 CCTAAGG CC^T[ACGT]AGG CelII 2653 GCTTAGC GC^T[ACGT]AGC DraIII 2833 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 2326 CGGCCG C^GGCCG EclXI 2326 CGGCCG C^GGCCG Eco52I 2326 CGGCCG C^GGCCG Eco81I 994 CCTAAGG CC^T[ACGT]AGG HincII 3094 GTTGAC GT[CT]^[GA]AC HindII 3094 GTTGAC GT[CT]^[GA]AC KspI 137 CCGCGG CCGC^GG MamI 1908 GATTCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC PflFI 2256 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 1860 CACGTG CAC^GTG PmlI 1860 CACGTG CAC^GTG PvuI 1287 CGATCG CGAT^CG RcaI 1583 TCATGA T^CATGA SacII 137 CCGCGG CCGC^GG ScaI 1962 AGTACT AGT^ACT SspI 2570 AATATT AAT^ATT Tth111I 2256 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AcyI 110 GGCGTC G[GA]^CG[CT]C 816 GACGCC AflIII 1622 ACACGT A^C[GA][CT]GT 2107 ACGTGT BcgI 1180 GCAGCGGTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1545 GCAGAAGAGTCG BglII 1394 AGATCT A^GATCT 3468 AGATCT BsaAI 1623 CACGTA [CT]AC^GT[GA] 1860 CACGTG BsaHI 110 GGCGTC G[GA]^CG[CT]C 816 GACGCC BstAPI 1073 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2079 GCAGAGGCTGC BstXI 2487 CCAGAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3399 CCACAGAGGTGG DrdI 940 GACACCTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2152 GACCCCTGAGTC EcoRI 2677 GAATTC G^AATTC 3344 GAATTC Hin1I 110 GGCGTC G[GA]^CG[CT]C 816 GACGCC HindIII 3353 AAGCTT A^AGCTT 3648 AAGCTT NcoI 1347 CCATGG C^CATGG 2348 CCATGG SacI 8 GAGCTC GAGCT^C 2490 GAGCTC SapI 2058 GAAGAGC GCTCTTC(1/4) 3047 GCTCTTC SphI 1743 GCATGC GCATG^C 1901 GCATGC StuI 684 AGGCCT AGG^CCT 3118 AGGCCT TaqI 1916 TCGA T^CGA 1986 TCGA TthHB8I 1916 TCGA T^CGA 1986 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 117 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI ApaI ApoI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BclI BcnI BfaI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I SbfI ScrFI SfaNI SfcI SmlI Sse8387I StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XhoII