##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BamHI BbeI
BfrI BglI BlnI BsaI BseAI
BsiWI BsmI Bsp1407I BspDI BspEI
BspLU11I BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraI Eam1105I Eco47III EcoO65I EcoRV
EcoT22I FseI FspI HaeII HpaI
KasI KpnI MfeI MluI MroI
MunI NarI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI PshAI PshBI Psp1406I
RsrII SalI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SspBI SwaI XbaI XcmI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AccI 898 GTCTAC GT^[AC][GT]AC
AocI 994 CCTAAGG CC^T[ACGT]AGG
AspI 2256 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 1860 CACGTG CAC^GTG
BbvCI 3234 CCTCAGC CCTCAGC(-5/-2)
BciVI 3432 GTATCC GTATCC(6/5)
BlpI 2653 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 2653 GCTTAGC GC^T[ACGT]AGC
BsaBI 1908 GATTCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 298 ACCGGA [AT]^CCGG[AT]
BsmBI 490 GAGACG CGTCTC(1/5)
BspHI 1583 TCATGA T^CATGA
BsrBI 868 GAGCGG CCGCTC(-3/-3)
BssSI 29 CTCGTG CACGAG(-5/-1)
Bsu36I 994 CCTAAGG CC^T[ACGT]AGG
CelII 2653 GCTTAGC GC^T[ACGT]AGC
DraIII 2833 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 2326 CGGCCG C^GGCCG
EclXI 2326 CGGCCG C^GGCCG
Eco52I 2326 CGGCCG C^GGCCG
Eco81I 994 CCTAAGG CC^T[ACGT]AGG
HincII 3094 GTTGAC GT[CT]^[GA]AC
HindII 3094 GTTGAC GT[CT]^[GA]AC
KspI 137 CCGCGG CCGC^GG
MamI 1908 GATTCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PflFI 2256 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1860 CACGTG CAC^GTG
PmlI 1860 CACGTG CAC^GTG
PvuI 1287 CGATCG CGAT^CG
RcaI 1583 TCATGA T^CATGA
SacII 137 CCGCGG CCGC^GG
ScaI 1962 AGTACT AGT^ACT
SspI 2570 AATATT AAT^ATT
Tth111I 2256 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcyI 110 GGCGTC G[GA]^CG[CT]C
816 GACGCC
AflIII 1622 ACACGT A^C[GA][CT]GT
2107 ACGTGT
BcgI 1180 GCAGCGGTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1545 GCAGAAGAGTCG
BglII 1394 AGATCT A^GATCT
3468 AGATCT
BsaAI 1623 CACGTA [CT]AC^GT[GA]
1860 CACGTG
BsaHI 110 GGCGTC G[GA]^CG[CT]C
816 GACGCC
BstAPI 1073 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2079 GCAGAGGCTGC
BstXI 2487 CCAGAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3399 CCACAGAGGTGG
DrdI 940 GACACCTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2152 GACCCCTGAGTC
EcoRI 2677 GAATTC G^AATTC
3344 GAATTC
Hin1I 110 GGCGTC G[GA]^CG[CT]C
816 GACGCC
HindIII 3353 AAGCTT A^AGCTT
3648 AAGCTT
NcoI 1347 CCATGG C^CATGG
2348 CCATGG
SacI 8 GAGCTC GAGCT^C
2490 GAGCTC
SapI 2058 GAAGAGC GCTCTTC(1/4)
3047 GCTCTTC
SphI 1743 GCATGC GCATG^C
1901 GCATGC
StuI 684 AGGCCT AGG^CCT
3118 AGGCCT
TaqI 1916 TCGA T^CGA
1986 TCGA
TthHB8I 1916 TCGA T^CGA
1986 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApaI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BclI BcnI BfaI
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrDI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I SbfI ScrFI SfaNI
SfcI SmlI Sse8387I StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Van91I XhoII