################################## # NO CUT ENZYMES ( 103 enzymes) # ################################## Acc65I AccII AclI AflII AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BalI BbeI BbrPI BclI BfrI BglI BglII BlnI BsaBI BsiWI BsmBI BsmI Bsp1407I BspDI BspHI BsrBI BsrGI BssHII Bst1107I BstBI BstUI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I FbaI FseI FspI HaeII HgaI HpaI KasI KpnI KspI MamI MfeI MluI MluNI MscI MunI MvnI NaeI NarI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PmaCI PmeI PmlI PshBI Psp1406I RcaI RsrII SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AatII 1519 GACGTC GACGT^C AccI 1561 GTCTAC GT^[AC][GT]AC AccIII 938 TCCGGA T^CCGGA AcyI 1519 GACGTC G[GA]^CG[CT]C AflIII 2527 ACATGT A^C[GA][CT]GT AgeI 893 ACCGGT A^CCGGT BamHI 1214 GGATCC G^GATCC BbvCI 249 CCTCAGC CCTCAGC(-5/-2) BcgI 968 CGAGGCTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1810 GTATCC GTATCC(6/5) BlpI 370 GCTGAGC GC^T[ACGT]AGC Bpu1102I 370 GCTGAGC GC^T[ACGT]AGC BsaAI 1564 TACGTA [CT]AC^GT[GA] BsaHI 1519 GACGTC G[GA]^CG[CT]C BseAI 938 TCCGGA T^CCGGA BsgI 522 CTGCAC GTGCAG(16/14) BsiEI 1280 CGATCG CG[GA][CT]^CG BspEI 938 TCCGGA T^CCGGA BspLU11I 2527 ACATGT A^CATGT BspMI 156 ACCTGC ACCTGC(4/8) BsrFI 893 ACCGGT [GA]^CCGG[CT] BssSI 442 CTCGTG CACGAG(-5/-1) BstEII 152 GGTCACC G^GT[ACGT]ACC BstPI 152 GGTCACC G^GT[ACGT]ACC BstXI 1615 CCACAATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 370 GCTGAGC GC^T[ACGT]AGC Cfr10I 893 ACCGGT [GA]^CCGG[CT] EaeI 475 CGGCCA [CT]^GGCC[GA] EcoNI 2209 CCTTTAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 152 GGTCACC G^GT[ACGT]ACC EcoRI 1641 GAATTC G^AATTC EcoRV 1576 GATATC GAT^ATC EcoT22I 886 ATGCAT ATGCA^T FauI 1587 GCGGG CCCGC(4/6) Hin1I 1519 GACGTC G[GA]^CG[CT]C HincII 2392 GTCAAC GT[CT]^[GA]AC HindII 2392 GTCAAC GT[CT]^[GA]AC HindIII 1180 AAGCTT A^AGCTT MroI 938 TCCGGA T^CCGGA MspA1I 643 CAGCTG C[AC]G^C[GT]G NcoI 1493 CCATGG C^CATGG NdeI 1157 CATATG CA^TATG NsiI 886 ATGCAT ATGCA^T NspI 2527 ACATGT [GA]CATG^[CT] PinAI 893 ACCGGT A^CCGGT PpuMI 998 GGGTCCC [GA]G^G[AT]CC[CT] PshAI 2079 GACCCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 82 CTGCAG CTGCA^G PvuI 1280 CGATCG CGAT^CG PvuII 643 CAGCTG CAG^CTG SapI 919 GAAGAGC GCTCTTC(1/4) SexAI 423 ACCAGGT A^CC[AT]GGT SmaI 2548 CCCGGG CCC^GGG SnaBI 1564 TACGTA TAC^GTA XcmI 1871 CCATGACTTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 2548 CCCGGG C^CCGGG XmaI 2548 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AciI 2358 CCGC CCGC(-3/-1) 1587 GCGG Asp700I 201 GAATCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2378 GAAACCATTC AvaI 966 CCCGAG C^[CT]CG[GA]G 2548 CCCGGG BanI 745 GGCACC G^G[CT][GA]CC 1188 GGCACC BsoBI 966 CCCGAG C^[CT]CG[GA]G 2548 CCCGGG BsrDI 355 CATTGC GCAATG(2/0) 1864 GCAATG BstAPI 639 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2199 GCAGACTATGC CfoI 963 GCGC GCG^C 1032 GCGC HhaI 963 GCGC GCG^C 1032 GCGC HinP1I 963 GCGC G^CGC 1032 GCGC MslI 651 CACCGGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 759 CACCACTATG SacI 373 GAGCTC GAGCT^C 922 GAGCTC SfcI 82 CTGCAG C^T[GA][CT]AG 1829 CTGTAG SmlI 196 CTTGAG C^T[CT][GA]AG 750 CTCAAG TaqI 490 TCGA T^CGA 728 TCGA TfiI 201 GAATC G^A[AT]TC 755 GATTC TthHB8I 490 TCGA T^CGA 728 TCGA XmnI 201 GAATCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2378 GAAACCATTC ######################################### # 3 OR MORE CUT ENZYMES ( 80 enzymes) # ######################################### AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaII BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII DsaI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PflMI PleI RsaI Sau3AI Sau96I ScrFI SfaNI StyI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XhoII