##################################
# NO CUT ENZYMES ( 103 enzymes) #
##################################
Acc65I AccII AclI AflII AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BalI BbeI
BbrPI BclI BfrI BglI BglII
BlnI BsaBI BsiWI BsmBI BsmI
Bsp1407I BspDI BspHI BsrBI BsrGI
BssHII Bst1107I BstBI BstUI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I FbaI FseI FspI
HaeII HgaI HpaI KasI KpnI
KspI MamI MfeI MluI MluNI
MscI MunI MvnI NaeI NarI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PmaCI PmeI
PmlI PshBI Psp1406I RcaI RsrII
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AatII 1519 GACGTC GACGT^C
AccI 1561 GTCTAC GT^[AC][GT]AC
AccIII 938 TCCGGA T^CCGGA
AcyI 1519 GACGTC G[GA]^CG[CT]C
AflIII 2527 ACATGT A^C[GA][CT]GT
AgeI 893 ACCGGT A^CCGGT
BamHI 1214 GGATCC G^GATCC
BbvCI 249 CCTCAGC CCTCAGC(-5/-2)
BcgI 968 CGAGGCTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1810 GTATCC GTATCC(6/5)
BlpI 370 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 370 GCTGAGC GC^T[ACGT]AGC
BsaAI 1564 TACGTA [CT]AC^GT[GA]
BsaHI 1519 GACGTC G[GA]^CG[CT]C
BseAI 938 TCCGGA T^CCGGA
BsgI 522 CTGCAC GTGCAG(16/14)
BsiEI 1280 CGATCG CG[GA][CT]^CG
BspEI 938 TCCGGA T^CCGGA
BspLU11I 2527 ACATGT A^CATGT
BspMI 156 ACCTGC ACCTGC(4/8)
BsrFI 893 ACCGGT [GA]^CCGG[CT]
BssSI 442 CTCGTG CACGAG(-5/-1)
BstEII 152 GGTCACC G^GT[ACGT]ACC
BstPI 152 GGTCACC G^GT[ACGT]ACC
BstXI 1615 CCACAATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 370 GCTGAGC GC^T[ACGT]AGC
Cfr10I 893 ACCGGT [GA]^CCGG[CT]
EaeI 475 CGGCCA [CT]^GGCC[GA]
EcoNI 2209 CCTTTAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 152 GGTCACC G^GT[ACGT]ACC
EcoRI 1641 GAATTC G^AATTC
EcoRV 1576 GATATC GAT^ATC
EcoT22I 886 ATGCAT ATGCA^T
FauI 1587 GCGGG CCCGC(4/6)
Hin1I 1519 GACGTC G[GA]^CG[CT]C
HincII 2392 GTCAAC GT[CT]^[GA]AC
HindII 2392 GTCAAC GT[CT]^[GA]AC
HindIII 1180 AAGCTT A^AGCTT
MroI 938 TCCGGA T^CCGGA
MspA1I 643 CAGCTG C[AC]G^C[GT]G
NcoI 1493 CCATGG C^CATGG
NdeI 1157 CATATG CA^TATG
NsiI 886 ATGCAT ATGCA^T
NspI 2527 ACATGT [GA]CATG^[CT]
PinAI 893 ACCGGT A^CCGGT
PpuMI 998 GGGTCCC [GA]G^G[AT]CC[CT]
PshAI 2079 GACCCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 82 CTGCAG CTGCA^G
PvuI 1280 CGATCG CGAT^CG
PvuII 643 CAGCTG CAG^CTG
SapI 919 GAAGAGC GCTCTTC(1/4)
SexAI 423 ACCAGGT A^CC[AT]GGT
SmaI 2548 CCCGGG CCC^GGG
SnaBI 1564 TACGTA TAC^GTA
XcmI 1871 CCATGACTTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 2548 CCCGGG C^CCGGG
XmaI 2548 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AciI 2358 CCGC CCGC(-3/-1)
1587 GCGG
Asp700I 201 GAATCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2378 GAAACCATTC
AvaI 966 CCCGAG C^[CT]CG[GA]G
2548 CCCGGG
BanI 745 GGCACC G^G[CT][GA]CC
1188 GGCACC
BsoBI 966 CCCGAG C^[CT]CG[GA]G
2548 CCCGGG
BsrDI 355 CATTGC GCAATG(2/0)
1864 GCAATG
BstAPI 639 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2199 GCAGACTATGC
CfoI 963 GCGC GCG^C
1032 GCGC
HhaI 963 GCGC GCG^C
1032 GCGC
HinP1I 963 GCGC G^CGC
1032 GCGC
MslI 651 CACCGGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
759 CACCACTATG
SacI 373 GAGCTC GAGCT^C
922 GAGCTC
SfcI 82 CTGCAG C^T[GA][CT]AG
1829 CTGTAG
SmlI 196 CTTGAG C^T[CT][GA]AG
750 CTCAAG
TaqI 490 TCGA T^CGA
728 TCGA
TfiI 201 GAATC G^A[AT]TC
755 GATTC
TthHB8I 490 TCGA T^CGA
728 TCGA
XmnI 201 GAATCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2378 GAAACCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AcsI AfaI AluI AlwI AlwNI
ApoI AspHI AvaII BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaI BsaJI BsaWI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraII DsaI EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI RsaI Sau3AI Sau96I
ScrFI SfaNI StyI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XhoII