##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AocI Aor51HI AscI Asp718I AspI
AvaI AviII BalI BamHI BbrPI
BcgI BglI BsaAI BsaWI BseAI
BsiEI BsiWI BsmBI BsoBI BspDI
BspEI BspMI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I ClaI
DraIII DrdI EagI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRV FseI
FspI KpnI MfeI MluI MluNI
MroI MscI MunI NcoI NdeI
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
SacI SalI SapI SbfI ScaI
SfiI SfuI SgrAI SmaI SnaBI
SphI Sse8387I StuI TaqI Tth111I
TthHB8I Van91I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AcyI 8 GGCGCC G[GA]^CG[CT]C
AhdI 111 GACATCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 327 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 423 GGGCCC GGGCC^C
Asp700I 3293 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 111 GACATCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 3055 CCTAGG C^CTAGG
BanII 423 GGGCCC G[GA]GC[CT]^C
BbeI 8 GGCGCC GGCGC^C
BbvCI 1405 CCTCAGC CCTCAGC(-5/-2)
BclI 3322 TGATCA T^GATCA
BcnI 50 CCCGG CC^[GC]GG
BglII 2483 AGATCT A^GATCT
BlnI 3055 CCTAGG C^CTAGG
BlpI 284 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 284 GCTGAGC GC^T[ACGT]AGC
BsaBI 2885 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 8 GGCGCC G[GA]^CG[CT]C
BsaI 2201 GAGACC GGTCTC(1/5)
BseRI 304 GAGGAG GAGGAG(10/8)
BspLU11I 673 ACATGT A^CATGT
BsrBI 287 GAGCGG CCGCTC(-3/-3)
BsrDI 871 GCAATG GCAATG(2/0)
BssHII 167 GCGCGC G^CGCGC
BstXI 1930 CCAACCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 284 GCTGAGC GC^T[ACGT]AGC
CpoI 337 CGGACCG CG^G[AT]CCG
DsaI 127 CCGCGG C^C[GA][CT]GG
EaeI 323 CGGCCA [CT]^GGCC[GA]
Eam1105I 111 GACATCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 3322 TGATCA T^GATCA
HgaI 2165 GACGC GACGC(5/10)
Hin1I 8 GGCGCC G[GA]^CG[CT]C
HpaI 2009 GTTAAC GTT^AAC
KasI 8 GGCGCC G^GCGCC
KspI 127 CCGCGG CCGC^GG
MamI 2885 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 8 GGCGCC GG^CGCC
NciI 50 CCCGG CC^[GC]GG
RsrII 337 CGGACCG CG^G[AT]CCG
SacII 127 CCGCGG CCGC^GG
SexAI 1994 ACCAGGT A^CC[AT]GGT
SfoI 8 GGCGCC GGC^GCC
SpeI 2418 ACTAGT A^CTAGT
SwaI 706 ATTTAAAT ATTT^AAAT
XbaI 3249 TCTAGA T^CTAGA
XmnI 3293 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 677 GTCTAC GT^[AC][GT]AC
2775 GTAGAC
AflIII 619 ACACGT A^C[GA][CT]GT
673 ACATGT
Alw44I 458 GTGCAC G^TGCAC
2870 GTGCAC
ApaLI 458 GTGCAC G^TGCAC
2870 GTGCAC
BanI 8 GGCGCC G^G[CT][GA]CC
2075 GGCACC
BciVI 381 GTATCC GTATCC(6/5)
1398 GTATCC
BsgI 1426 CTGCAC GTGCAG(16/14)
3404 CTGCAC
Bsp1407I 1529 TGTACA T^GTACA
3498 TGTACA
BspHI 1688 TCATGA T^CATGA
2197 TCATGA
BsrGI 1529 TGTACA T^GTACA
3498 TGTACA
EarI 2360 CTCTTC CTCTTC(1/4)
761 GAAGAG
EcoRI 2131 GAATTC G^AATTC
2987 GAATTC
EcoT14I 1168 CCTTGG C^C[AT][AT]GG
3055 CCTAGG
EcoT22I 2680 ATGCAT ATGCA^T
3026 ATGCAT
HaeII 8 GGCGCC [GA]GCGC^[CT]
1339 AGCGCC
HindIII 1324 AAGCTT A^AGCTT
1598 AAGCTT
Ksp632I 2360 CTCTTC CTCTTC(1/4)
761 GAAGAG
NaeI 193 GCCGGC GCC^GGC
277 GCCGGC
NgoMIV 193 GCCGGC G^CCGGC
277 GCCGGC
NsiI 2680 ATGCAT ATGCA^T
3026 ATGCAT
NspI 673 ACATGT [GA]CATG^[CT]
3155 GCATGT
PpuMI 244 GGGACCC [GA]G^G[AT]CC[CT]
263 GGGTCCC
RcaI 1688 TCATGA T^CATGA
2197 TCATGA
SspBI 1529 TGTACA T^GTACA
3498 TGTACA
StyI 1168 CCTTGG C^C[AT][AT]GG
3055 CCTAGG
XcmI 65 CCAGGGAAGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2046 CCAACAACCGCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccII AciI AcsI AfaI AflII
AluI AlwI ApoI AseI AsnI
AspHI AvaII BbsI BbvI BfaI
BfrI BmyI BpmI BpuAI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BsrFI BsrI BssKI
BssSI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII EcoNI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NdeII NlaIII
NlaIV PleI PshBI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII