##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AccIII AclI AgeI AhdI Alw44I
Aor51HI ApaLI AscI AspEI AviII
AvrII BamHI BbrPI BbvCI BcgI
BciVI BglI BlnI BlpI Bpu1102I
BsaI BsaWI BseAI BsiWI BspDI
BspEI BspMI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
CelII ClaI CpoI DrdI Eam1105I
Eco47III EcoNI EcoO65I EcoRV FseI
FspI HgaI MluI MroI NcoI
NotI NruI NspV PaeR7I PflMI
PinAI PmaCI PmeI PmlI PpuMI
Psp1406I PvuI RsrII SalI SapI
SbfI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I StuI SwaI
Van91I XcmI XhoI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AatII 258 GACGTC GACGT^C
Acc65I 3672 GGTACC G^GTACC
AccI 261 GTCTAC GT^[AC][GT]AC
AflII 1196 CTTAAG C^TTAAG
AlwNI 2849 CAGTTACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1642 CCTTAGG CC^T[ACGT]AGG
ApaI 165 GGGCCC GGGCC^C
Asp718I 3672 GGTACC G^GTACC
AvaII 489 GGACC G^G[AT]CC
BbeI 94 GGCGCC GGCGC^C
BclI 3767 TGATCA T^GATCA
BfrI 1196 CTTAAG C^TTAAG
BsaAI 480 CACGTA [CT]AC^GT[GA]
BseRI 26 CTCCTC GAGGAG(10/8)
BsiEI 87 CGGCCG CG[GA][CT]^CG
BsmBI 2436 GAGACG CGTCTC(1/5)
BsrBI 120 GAGCGG CCGCTC(-3/-3)
BsrDI 3289 GCAATG GCAATG(2/0)
BsrFI 85 GCCGGC [GA]^CCGG[CT]
BstXI 1667 CCAGTTAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1642 CCTTAGG CC^T[ACGT]AGG
Cfr10I 85 GCCGGC [GA]^CCGG[CT]
DraII 164 GGGGCCC [GA]G^G[ACGT]CC[CT]
DraIII 915 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 90 CCGCGG C^C[GA][CT]GG
EagI 87 CGGCCG C^GGCCG
EarI 2459 CTCTTC CTCTTC(1/4)
EclXI 87 CGGCCG C^GGCCG
Eco52I 87 CGGCCG C^GGCCG
Eco81I 1642 CCTTAGG CC^T[ACGT]AGG
EcoO109I 164 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoT22I 1515 ATGCAT ATGCA^T
FbaI 3767 TGATCA T^GATCA
HaeII 94 GGCGCC [GA]GCGC^[CT]
KasI 94 GGCGCC G^GCGCC
KpnI 3672 GGTACC GGTAC^C
Ksp632I 2459 CTCTTC CTCTTC(1/4)
KspI 90 CCGCGG CCGC^GG
NaeI 85 GCCGGC GCC^GGC
NarI 94 GGCGCC GG^CGCC
NgoMIV 85 GCCGGC G^CCGGC
NheI 2663 GCTAGC G^CTAGC
NsiI 1515 ATGCAT ATGCA^T
PacI 2301 TTAATTAA TTAAT^TAA
PshAI 490 GACCTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 721 CAGCTG CAG^CTG
SacI 1658 GAGCTC GAGCT^C
SacII 90 CCGCGG CCGC^GG
ScaI 1891 AGTACT AGT^ACT
SfoI 94 GGCGCC GGC^GCC
SmaI 139 CCCGGG CCC^GGG
XbaI 2884 TCTAGA T^CTAGA
XmaCI 139 CCCGGG C^CCGGG
XmaI 139 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 94 GGCGCC G[GA]^CG[CT]C
258 GACGTC
Asp700I 677 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3802 GAAGTGGTTC
AspHI 1658 GAGCTC G[AT]GC[AT]^C
2242 GAGCAC
AvaI 2 CCCGAG C^[CT]CG[GA]G
139 CCCGGG
BalI 1172 TGGCCA TGG^CCA
3894 TGGCCA
BglII 1281 AGATCT A^GATCT
2703 AGATCT
BpmI 709 CTCCAG CTGGAG(16/14)
2517 CTGGAG
BsaHI 94 GGCGCC G[GA]^CG[CT]C
258 GACGTC
BsgI 646 CTGCAC GTGCAG(16/14)
345 GTGCAG
BsiHKAI 1658 GAGCTC G[AT]GC[AT]^C
2242 GAGCAC
BsmI 2683 GAATGC GAATGC(1/-1)
1517 GCATTC
BsoBI 2 CCCGAG C^[CT]CG[GA]G
139 CCCGGG
BspLU11I 3567 ACATGT A^CATGT
4181 ACATGT
DraI 3366 TTTAAA TTT^AAA
4143 TTTAAA
EcoRI 1114 GAATTC G^AATTC
3238 GAATTC
Hin1I 94 GGCGCC G[GA]^CG[CT]C
258 GACGTC
HindIII 426 AAGCTT A^AGCTT
2032 AAGCTT
HpaI 279 GTTAAC GTT^AAC
2878 GTTAAC
MluNI 1172 TGGCCA TGG^CCA
3894 TGGCCA
MscI 1172 TGGCCA TGG^CCA
3894 TGGCCA
PleI 1352 GAGTC GAGTC(4/5)
3744 GAGTC
PstI 534 CTGCAG CTGCA^G
2943 CTGCAG
SexAI 46 ACCAGGT A^CC[AT]GGT
4013 ACCAGGT
SfcI 534 CTGCAG C^T[GA][CT]AG
2943 CTGCAG
SmlI 1196 CTTAAG C^T[CT][GA]AG
1359 CTCAAG
SspI 387 AATATT AAT^ATT
3228 AATATT
XmnI 677 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3802 GAAGTGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI ApoI AseI AsnI
AspI BanI BanII BbsI BbvI
BcnI BfaI BmyI BpuAI BsaBI
BsaJI BsiYI BslI BsmAI BsmFI
Bsp1286I Bsp1407I BspHI BsrGI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
EaeI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MamI
MboI MboII MfeI MflI MnlI
MseI MslI MspA1I MspI MunI
MvaI MvnI MwoI NciI NdeI
NdeII NlaIII NlaIV NspI PflFI
PshBI RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I XhoII